Search results for "Citosquelet"
showing 3 items of 3 documents
Evolutionary conserved role of eukaryotic translation factor eIF5A in the regulation of actin-nucleating formins
2017
AbstractElongation factor eIF5A is required for the translation of consecutive prolines, and was shown in yeast to translate polyproline-containing Bni1, an actin-nucleating formin required for polarized growth during mating. Here we show that Drosophila eIF5A can functionally replace yeast eIF5A and is required for actin-rich cable assembly during embryonic dorsal closure (DC). Furthermore, Diaphanous, the formin involved in actin dynamics during DC, is regulated by and mediates eIF5A effects. Finally, eIF5A controls cell migration and regulates Diaphanous levels also in mammalian cells. Our results uncover an evolutionary conserved role of eIF5A regulating cytoskeleton-dependent processes…
Proteómica del endometrio humano
2012
La presente tesis doctoral se centra en el estudio del endometrio, el cual desarrolla temporalmente un estado en el que se permite la adhesión del blastocisto después de la fecundación, para posibilitar la implantación. Además este tejido permite que se produzca la posterior invasión controlada del trofoblasto que dará lugar a la placentación, que resulta imprescindible para el desarrollo fetal. Todos esos cambios, tanto a nivel morfológico como molecular, están regulados a nivel proteico y por ello, caracterizar y conocer el proteoma podría ser utilizado para mejorar el entendimiento de este proceso. Esta tesis persigue obtener el conjunto de proteínas diferencialmente expresadas del endom…
The Stress-Inducible Protein DRR1 Exerts Distinct Effects on Actin Dynamics.
2018
Cytoskeletal dynamics are pivotal to memory, learning, and stress physiology, and thus psychiatric diseases. Downregulated in renal cell carcinoma 1 (DRR1) protein was characterized as the link between stress, actin dynamics, neuronal function, and cognition. To elucidate the underlying molecular mechanisms, we undertook a domain analysis of DRR1 and probed the effects on actin binding, polymerization, and bundling, as well as on actin-dependent cellular processes. Methods: DRR1 domains were cloned and expressed as recombinant proteins to perform in vitro analysis of actin dynamics (binding, bundling, polymerization, and nucleation). Cellular actin-dependent processes were analyzed in trans…