Search results for "Citrange"
showing 10 items of 11 documents
The effects of meta-Topolin and benzyladenine on in vitro organogenesis from epicotyl cuttings of Troyer citrange (Citrus sinensis[L.] Osbeck ×Poncir…
2017
Citrus tristeza virus (CTV), worldwide considered as one of the worst disease affecting Citrus plants, produced great changes in citriculture, mainly because it made mandatory the turnover of the sensitive sour orange rootstock with virus resistant ones. Among them, Troyer citrange has become one of the most employed rootstocks, due to its CTV tolerance and to the positive characteristics induced to the scion fruit production. Unfortunately, in Italy, there is a scarcity of seed mother plants. Micropropagation can be a solution for this problem. Among the in vitro techniques allowing the production of good quality true-to-type plants, the regeneration from seedling epicotyl cuttings general…
Bot gummosis of lemon (Citrus × limon) caused by neofusicoccum parvum
2021
Neofusicoccum parvum, in the family Botryosphaeriaceae, was identified as the causal agent of bot gummosis of lemon (Citrus × limon) trees, in the two major lemon-producing regions in Italy. Gummy cankers on trunk and scaffold branches of mature trees were the most typical disease symptoms. Neofusicoccum parvum was the sole fungus constantly and consistently isolated from the canker bark of symptomatic lemon trees. It was identified on the basis of morphological characters and the phylogenetic analysis of three loci, i.e., the internal transcribed spacer of nuclear ribosomal DNA (ITS) as well as the translation elongation factor 1-alpha (TEF1) and β-tubulin (TUB2) genes. The pathogenicity o…
Preliminary investigations on the occurrence of Citrus Tristeza Virus (CTV)in central-western Sicilian orchards and on the mycorrhizal status in citr…
2013
In the last decade, since the first outbreak of Citrus tristeza virus (CTV) in the province of Catania (2002), several new CTV foci were found in Sicily. In order to determine CTV occurrence in centra-lwestern Sicily, identify and characterize viral strains and verify the mycorrhizal status of citrange [Citrus sinensis (L.) Osb. × Poncirus trifoliate (L.) Raf.]. one of the most utilized tollerant rootstock, an epidemiological survey was carried out in 2011. Symptomless samples from citrus groves from different Sicilian provinces (Agrigento, Palermo, Trapani, Caltanissetta) were collected and submitted to serological assay. Only samples from Agrigento and Palermo provinces were ELISA-positiv…
EX VITRO MYCORRHIZATION OF VITRO-DERIVED PLANTLETS OF 'CARRIZO' CITRANGE [C. SINENSIS (L.) OSB. × P. TRIFOLIATA (L.) RAF.]
2015
In this study, vitro-derived plantlets of âCarrizoâ citrange were inoculated with Glomus intraradices during the acclimatization phase, from in vitro to in vivo. Plantlets were grown in presence (+M) and in absence of inoculum (-M) and in addition, in sterile and non-sterile substrate. After three months of culture, some vegetative parameters and the level of infection, varied greatly in the different theses. In particular, the highest formation of new branched roots has been observed in inoculated plantlets cultivated in sterile substrate. Good results were observed also in non-inoculated plantlets cultivated in non-sterile substrate, in presence of endemic inoculum. The same trend was…
Ex vitro mycorrhization of vitro-derived plantlets of 'Carrizo' citrange [C. Sinensis (L.) Osb. × P. Trifoliata (L.) Raf.]
2015
In this study, vitro-derived plantlets of 'Carrizo' citrange were inoculated with Glomus intraradices during the acclimatization phase, from in vitro to in vivo. Plantlets were grown in presence (+M) and in absence of inoculum (-M) and in addition, in sterile and non-sterile substrate. After three months of culture, some vegetative parameters and the level of infection, varied greatly in the different theses. In particular, the highest formation of new branched roots has been observed in inoculated plantlets cultivated in sterile substrate. Good results were observed also in non-inoculated plantlets cultivated in non-sterile substrate, in presence of endemic inoculum. The same trend was obs…
Preliminary Results on Biotization of Encapsulated Vitro-Derived Propagules of Carrizo Citrange [Citrus sinensis (L.) Osb. × Poncirus trifoliata (L.)…
2015
The encapsulation technology represents a new tool to integrate micropropagation into the nursery activity. It allows combining the advantages of zygotic or gamic seeds with those of micropropagation. The synthetic or artificial seeds have been defined as “artificially encapsulated somatic embryos, shoots or other tissues which can be used for sowing under in vitro or ex vitro conditions”. This will be a powerful propagation tool in the nurseryman hands, if the levels of the synthetic seeds conversion can be increased also in the nurseries, without the asepsis of in vitro laboratories and with the presence of many parasitic microorganisms, like bacteria and fungi, responsible for contaminat…
The effects of benzyladenine and meta-Topolin on in vitro sprouting and regrowth after encapsulation of C35 citrange [Citrus sinensis (L.) Osb. × Pon…
2016
C35, a hybrid of ‘Ruby Blood’ sweet orange and trifoliate orange, is a promising Citrus rootstock, mainly due to its resistance to Citrus Tristeza Virus (CTV), one of the most serious pathology affecting citriculture. The use of resistant rootstocks, such as citranges, is an efficient tool to fight this plant disease. This implies a continuous increasing demand of resistant rootstocks, in order to carry out the turnover of sour orange, still the most used Citrus rootstock in Italy. Micropropagation, can provide innovative solutions to obtain, in a short time and in a small space, a number of plants higher than traditional propagation. Micropropagation efficiency is also affected by the cult…
Sull’impiego di inoculi AM in ambito vivaistico
2014
Molecular characterization of citrus tristeza virus (CTV) isolates and detection of fungi associated with Citrange root-stock in Sicilian symptomless…
2014
The first monitoring on the CTV occurrence in central-western Sicily carried out during spring 2011 in asymptomatic citrus groves, has allowed to report CTV- free citrus-growing areas (province of Caltanissetta and Trapani), the first focus in province of Agrigento and two new others in province of Palermo. Molecular analysis (RT-PCR of p18, p20 and p23 genes, single strand conformation polymorphism and partial sequencing) showed that the obtained CTV isolates had a high homology (99%) with mild CTV strains (T 30 and T 385). Moreover, survey on the genetic flow and analysis of synonymous/nonsynonymous, by MEGA 5.0 and DNAsp 5.0 programs, demonstrated the provenience of the new “tristeza” fo…