Search results for "Clamp"
showing 10 items of 243 documents
Mechanical and electrophysiological effects of cromakalim on the human urinary bladder.
1994
The effects of cromakalim on spontaneous and induced mechanical activity of human detrusor muscle were investigated in vitro. Cromakalim produces a concentration-related decrease of spontaneous as well as carbachol- and K(+)-evoked contractions. This is the first study to utilize the patch clamp technique to elucidate the mechanism of action of cromakalim on human detrusor cells. Cromakalim hyperpolarizes the detrusor cells by increasing the net outward current which is most likely carried by potassium ions. In the human urinary bladder, this effect is mediated by a glibenclamide-sensitive potassium channel, as glibenclamide is able to diminish the relaxant effect of cromakalim and to preve…
Extracellular site of action of phenylalkylamines on L-type calcium current in rat ventricular myocytes.
1995
The effects of the phenylalkylamines verapamil, gallopamil, and devapamil on L-type calcium currents (ICa) were studied in ventricular myocytes from rat hearts using the whole-cell patch-clamp technique. In particular, the question was addressed, whether the pharmacological binding sites for these drugs were located at the inner and/or at the outer surface of the cell membrane. Therefore, tertiary verapamil, gallopamil, and devapamil and their corresponding quaternary derivatives were applied either from the outside or the inside of the cell membrane. Extracellular application of verapamil, gallopamil and devapamil (each at 3 microM) reduced ICa to 16.1 +/- 8.6%, 11 +/- 8.9%, and 9.3 +/- 6%…
Functional Integration of Neuronal Precursors in the Adult Murine Piriform Cortex
2018
Abstract The extent of functional maturation and integration of nonproliferative neuronal precursors, becoming neurons in the adult murine piriform cortex, is largely unexplored. We thus questioned whether precursors eventually become equivalent to neighboring principal neurons or whether they represent a novel functional network element. Adult brain neuronal precursors and immature neurons (complex cells) were labeled in transgenic mice (DCX-DsRed and DCX-CreERT2 /flox-EGFP), and their cell fate was characterized with patch clamp experiments and morphometric analysis of axon initial segments. Young (DCX+) complex cells in the piriform cortex of 2- to 4-month-old mice received sparse synapt…
Measuring of Geometrical Precision of Some parts Obtained by Asymmetric Incremental Forming Process After Trimming
2007
Asymmetric Incremental Forming exalts the advantages of Incremental Forming process since no dies are strictly necessary. In this way complex geometries may be manufactured with a very simple clamping equipment. On the other hand, this characteristic determines some intrinsic drawbacks which penalise its industrial suitability; first of all, the dimensional control of the manufactured part is a still open point for researchers all over the world. Several approaches have been already proposed in the last years to solve the problem, resulting only in partial solutions. At the same time, up to now, the numerical simulation did not supply significant aid to the designers, due to the problem com…
Shape Distortion and Thickness Distribution during SPIF Processes: Expermental and Numerical Analysis
2011
Single Point Incremental Forming (SPIF) is a quite new sheet forming process which offers the possibility to deform complex parts without dedicated dies using only a single point tool and a standard 3-axis CNC machine. The process mechanics enables strains much higher than traditional sheet forming processes, but particular attention must be given to the final part geometrical accuracy. In this paper the capabilities of a dedicated explicit numerical model are quantitatively analyzed on pyramid-shaped parts. In particular a comparison between experimental and numerical results is reported. Three different shapes at the varying of the stamping angle were considered and the final shape was ac…
Friction stir welding of tailored joints for industrial applications
2009
Friction stir welding (FSW) is an energy efficient and environmentally "friendly" (no fumes, noise, or sparks) welding process, during which the workpieces are welded together in a solid-state joining process at a temperature below the melting point of the workpiece material under a combination of extruding and forging. Since its invention in 1991 by TWI, such process has been reaching a continuously increasing popularity among aerospace, automotive and shipbuilding industries due its capability to weld unweldable or difficult-to-weld light alloys in different joint morphologies. In this paper a wide experimental campaign is carried out in order to obtain T and lap joints characterized by d…
FSW of Lap and T-Joints
2010
Even if in the last years several researches have studied the Friction Stir Welding (FSW) process, it should be observed that most of these studies are concerned with the butt joint and just a few of them extend to more complex geometries. It is worthy to notice that the acquired knowledge on FSW process of butt joints is not immediately extendable to lap and T-joints. The first observation is that in butt joints the surface to be welded is vertical, while in lap and T-joints it is horizontal and placed at the bottom of the top blank to be welded; in this way a major vertical component of the material flow is required to obtain sound joints. In the FSW of lap-joints four different geometric…
Dendrites are dispensable for basic motoneuron function but essential for fine tuning of behavior.
2014
Dendrites are highly complex 3D structures that define neuronal morphology and connectivity and are the predominant sites for synaptic input. Defects in dendritic structure are highly consistent correlates of brain diseases. However, the precise consequences of dendritic structure defects for neuronal function and behavioral performance remain unknown. Here we probe dendritic function by using genetic tools to selectively abolish dendrites in identified Drosophila wing motoneurons without affecting other neuronal properties. We find that these motoneuron dendrites are unexpectedly dispensable for synaptic targeting, qualitatively normal neuronal activity patterns during behavior, and basic …
An improved Flyback converter
2009
This paper presents a modified Flyback converter able to operate with higher efficiency and smaller size, overcoming most of the conventional flyback converter drawbacks, and also keeping its low cost and simplicity. In particular, the proposed converter allows the mosfet off voltage to be reduced and clamped to the input, thus recovering the transformer leakage energy. As a consequence, the duty cycle can be extended to unity, thus reducing the voltage stress across the output rectifier, and lowering both the magnetizing inductance and the transformer bias current values. In addition, due to the auxiliary mosfet, the magnetizing current can become negative, eliminating the discontinuous co…
A demagnetization circuit for forward converters
2009
This paper presents a demagnetization circuit able to operate the forward converter with an higher efficiency and with a wider input range. In particular, by the means of few added components, the magnetizing energy and the leakage one are continuously recovered, and the transformer reset and the primary mosfet OFF voltage clamp to the input, both under steady state and transient operations, are provided. The reduced voltage stress allows the converter to operate with a duty extended to unity over the typical 70% limit of the Active Clamp topology, with smaller output inductance, better converter dynamics and wider input range. Furthermore, thanks to this technique, by employing a duty appr…