Search results for "Clase"
showing 10 items of 319 documents
PACAP38 and PAC1 receptor blockade: a new target for headache?
2018
Abstract Pituitary adenylate cyclase activating polypeptide-38 (PACAP38) is a widely distributed neuropeptide involved in neuroprotection, neurodevelopment, nociception and inflammation. Moreover, PACAP38 is a potent inducer of migraine-like attacks, but the mechanism behind this has not been fully elucidated. Migraine is a neurovascular disorder, recognized as the second most disabling disease. Nevertheless, the antibodies targeting calcitonin gene-related peptide (CGRP) or its receptor are the only prophylactic treatment developed specifically for migraine. These antibodies have displayed positive results in clinical trials, but are not effective for all patients; therefore, new pharmacol…
Nitric Oxide System and Bronchial Epithelium: More Than a Barrier
2021
Airway epithelium forms a physical barrier that protects the lung from the entrance of inhaled allergens, irritants, or microorganisms. This epithelial structure is maintained by tight junctions, adherens junctions and desmosomes that prevent the diffusion of soluble mediators or proteins between apical and basolateral cell surfaces. This apical junctional complex also participates in several signaling pathways involved in gene expression, cell proliferation and cell differentiation. In addition, the airway epithelium can produce chemokines and cytokines that trigger the activation of the immune response. Disruption of this complex by some inflammatory, profibrotic, and carcinogens agents c…
Nitric oxide/cGMP signaling via guanylyl cyclase isoform 1 modulates glutamate and GABA release in somatosensory cortex of mice
2017
Abstract In hippocampus, two guanylyl cyclases (NO-GC1 and NO-GC2) are involved in the transduction of the effects of nitric oxide (NO) on synaptic transmission. However, the respective roles of the NO-GC isoforms on synaptic transmission are less clear in other regions of the brain. In the present study, we used knock-out mice deficient for the NO-GC1 isoform (NO-GC1 KO) to analyze its role in the glutamatergic and GABAergic neurotransmission at pyramidal neurons in layers II/III of somatosensory cortex. NO-GC1 KO slices revealed reduced frequencies of miniature excitatory- and inhibitory-postsynaptic currents, increased paired-pulse ratios and decreased input–output curves of evoked signa…
CD38-Specific Biparatopic Heavy Chain Antibodies Display Potent Complement-Dependent Cytotoxicity Against Multiple Myeloma Cells
2018
CD38 is overexpressed by multiple myeloma cells and has emerged as a target for therapeutic antibodies. Nanobodies are soluble single domain antibody fragments derived from the VHH variable domain of heavy chain antibodies naturally occurring in camelids. We previously identified distinct llama nanobodies that recognize three non-overlapping epitopes of the extracellular domain of CD38. Here, we fused these VHH domains to the hinge, CH2, and CH3 domains of human IgG1, yielding highly soluble chimeric llama/human heavy chain antibodies (hcAbs). We analyzed the capacity of these hcAbs to mediate complement-dependent cytotoxicity (CDC) to CD38-expressing human multiple myeloma and Burkitt lymp…
The link between bone microenvironment and immune cells in multiple myeloma: Emerging role of CD38
2018
The relationship between bone and immune cells is well established both in physiological and pathological conditions. Multiple myeloma (MM) is a plasma cell malignancy characterized by an increase of number and activity of osteoclasts (OCLs) and a decrease of osteoblasts (OBs). These events are responsible for bone lesions of MM patients. OCLs support MM cells survival in vitro and in vivo. Recently, the possible role of OCLs as immunosuppressive cells in the MM BM microenvironment has been underlined. OCLs protect MM cells against T cell-mediated cytotoxicity through the expression of several molecules including programmed death-ligand (PD-L) 1, galectin (Gal) 9, CD200, and indoleamine-2,3…
Robust and sensitive GFP-based cGMP sensor for real time imaging in intact Caenorhabditis elegans
2018
AbstractcGMP is a ubiquitous second messenger that plays a role in sensory signaling and plasticity through its regulation of ion channels and kinases. Previous studies that primarily used genetic and biochemical tools suggest that cGMP is spatiotemporally regulated in multiple sensory modalities, including light, heat, gases, salt and odor. FRET- and GFP-based cGMP sensors were developed to visualize cGMP in primary cell culture and Caenorhabditis elegans to corroborate these findings. While a FRET-based sensor has been used in an intact animal to visualize cGMP, the requirement of a multiple emission system limits its ability to be used on its own as well as with other sensors and fluores…
Validation of a questionnaire of attributions to the detection of coincidences in academic work
2019
El plagio como tema de investigación en el contexto universitario no ha cesado de ser una preocupación constante debido a sus múltiples repercusiones. El presente estudio describe el proceso seguido en la validación del Cuestionario de atribuciones para la detección de coincidencias en trabajos académicos (CUDECO), cuya finalidad es evaluar la relevancia que tiene citar correctamente y detectar las causas que llevan a cometer plagio. A partir de una muestra de 2,331 estudiantes, se realiza un anális is de la fiabilidad como consistencia interna y un análisis factorial exploratorio (AFE) del instrumento. El análisis de la fiabilidad lleva a proponer un reajuste en el cuestionario inicialment…
La vida de trabajo, el trabajo de la vida
2011
Análisis de las fotografías que revelan la vida cotidiana y las formas laborales,culturales y educativas de los jóvenes obreros de la planta siderúrgica del Puerto de Sagunto en el periodo de la dictadura franquista
Perfiles de vulnerabilidad social en la Ciudad de Valencia. Análisis desde la perspectiva sociológica de la pobreza
2021
En esta tesis doctoral se aborda el fenómeno de la pobreza desde una perspectiva sociológica. El impacto de la crisis financiera desatada en el año 2008 en la sociedad española, y en la valenciana, se expresó en el deterioro de las condiciones de vida en cada vez mayor número de hogares. Según el indicador AROPE en el año 2018, diez años después de iniciada la crisis, el 26% de la población en España se encontraba en riesgo de pobreza o exclusión social. A partir de la propuesta tipológica de S. Paugam sobre las Formas Elementales de la Pobreza he indagado en dos dimensiones de análisis: a) Las Representaciones Sociales de la Pobreza en España; y b) La heterogeneidad de las características …
2004
We investigated how the synthesis of cAMP, stimulated by isoproterenol acting through β-adrenoreceptors and Gs, is strongly amplified by simultaneous incubation with baclofen. Baclofen is an agonist of δ-aminobutyric acid type B receptors [GABAB], known to inhibit adenylyl cyclase via Gi. Because these agents have opposite effects on cAMP levels, the unexpected increase in cAMP synthesis when they are applied simultaneously has been intensively investigated. From previous reports, it appears that cyclase type II contributes most significantly to this phenomenon. We found that simultaneous application of isoproterenol and baclofen specifically influences the association/dissociation of molec…