Search results for "Clone"
showing 10 items of 312 documents
Long-term impacts of tropical storms and earthquakes on human population growth in Haiti and Dominican Republic
2010
AbstractThe two Caribbean states, Haiti and the Dominican Republic, have experienced similar natural forces since the 18th century, including hurricanes and earthquakes. Although, both countries seem to be two of the most prone of all Latin American and Caribbean countries to natural hazard events, historically, Haiti tends to be more vulnerable to natural forces. The purpose of this article is to understand to what extent geohazards shape demographic changes. Research findings of this study show that neither atmospheric nor seismic hazards that directly hit the territory of Haiti have significant impacts on population densities and growth rates. Quantitative evidence is provided, that betw…
Sharpness of the extratropical tropopause in baroclinic life cycle experiments
2007
[1] The sharpness of the extratropical tropopause is investigated using baroclinic life cycle experiments. In these simulations, slow synoptic scale dynamics leads to a net tropopause sharpening. The mechanism involves vertical convergence of the ageostrophic wind. The results support earlier suggestions from idealized theoretical studies.
Winter “weekend effect” in southern Europe and its connections with periodicities in atmospheric dynamics
2008
[1] Winter weekly cycles of different climatic variables have been detected over Spain during the 1961–2004 period. The 13 analyzed series come from stations placed on different climatological and geographical areas with different level of urban influence. Therefore, the weekly cycles can hardly be related with local effects. Contrarily, we suggest that the weekly cycles may be related with changes in the atmospheric circulation over Western Europe, which may be due to some indirect effect of anthropogenic aerosols. Particularly interesting is the observed increase in Sea Level Pressure over Southern Europe during the weekends and consequently a decrease of anticyclonic conditions during th…
Metagenomic analysis of the microbiota hindgut of Reticulitermes lucifugus (Rossi) (Isoptera: Rhinotermitidae)
2008
Termites hindguts host a microbial community consisting of anaerobic flagellates and bacteria. This gut microflora is responsible for the degradation of lignocellulosic material. Here we report the data concerning the analysis of the hindgut microbial community of Reticulitermes lucifugus (Rossi), a termite commonly present in Italy, based on a metagenomic approach. Metagenomic DNA was extracted from the gut content of termites and the SSU rDNA was polymerase chain reaction (PCR) - amplified using universal primers for prokaryotes and eukaryotes. Two clone libraries were constructed and clones were grouped into Operational Taxonomic Units (OTU) by RFLP analysis. One representative clone was…
In vitro production of anti-neutrophilocyte-cytoplasm-antibodies (ANCA) by Epstein-Barr virus-transformed B-cell lines in Wegener's granulomatosis.
1991
The frequent detection of anti-neutrophilocyte-cytoplasm-antibodies (ANCA) in patients with Wegener's granulomatosis (WG) led to the supposition that this disease might be of autoimmune nature. For some authors assume that Epstein-Barr virus (EBV) infection of human B-lymphocytes besides polyclonal activation could reveal the cryptic immune status against different autoantigens in patients with autoimmune diseases we investigated EBV-transformed B-lymphocytes from patients with Sjögren's syndrome, mixed connective tissue disease, WG and healthy blood donors. Two stable B-cell lines (Ho3, We1) could be established. Inhibition experiments showed that antibodies produced by transformed B-lymph…
Zebrafish vimentin: molecular characterization, assembly properties and developmental expression
1998
To provide a basis for the investigation of the intermediate filament (IF) protein vimentin in one of the most promising experimental vertebrate systems, the zebrafish (Danio rerio), we have isolated a cDNA clone of high sequence identity to and with the characteristic features of human vimentin. Using this clone we produced recombinant zebrafish vimentin and studied its assembly behaviour. Unlike other vimentins, zebrafish vimentin formed unusually thick filaments when assembled at temperatures below 21 degrees C. At 37 degrees C few filaments were observed, which often also terminated in aggregated masses, indicating that its assembly was severely disturbed at this temperature. Between 21…
Werewolves and warning signs: Cultural responses to tropical cyclones in Mauritius
2022
The role that culture plays in the way different groups experience, respond to, and recover from disasters has been widely discussed. Yet, while there is a considerable (and growing) literature of case study evidence for the need to account for culture in disasters, comparatively few studies take a long-term perspective on cultural interactions with disasters, resulting in a lack of exploration into the diachronic nature of these cultural responses, both past and present. The literature that does exist tends also to focus either on western cultures or on groups that pursue highly traditional livelihoods. Communities that call on elements of both local or vernacular knowledge and scientific …
Improving Separation of Oil and Water Using a Novel Coalescing Centrifugal Pump
2017
Abstract A novel centrifugal pump, which increases oil droplet sizes in produced water, has been developed. This paper investigates a concept of pumping pressure optimization with respect to downstream separation efficiency, for the new pump. The investigation shows that the coalescing centrifugal pump always increases the separation efficiency of a downstream hydrocyclone. Furthermore, it is seen that the pumping pressure can be adjusted to maximize the improvement. Experimental results demonstrate how pumping conditions, which minimize the volume fraction of droplets with a diameter smaller than the cut size of the hydrocyclone, maximize the separation efficiency. Finally, it is exemplifi…
Variable Step Size P&O Algorithms for Coalescing Pump/Deoiling Hydrocyclone Produced Water Treatment System
2020
This paper presents three variable step size P&O algorithms for optimizing the separation efficiency of a coalescing pump/deoiling hydrocyclone produced water treatment system. By continuously adjusting the pumping pressure, and subsequently the coalescing effect, the algorithms are used to minimize the oil concentration downstream the hydrocyclone. Due to the variable step size, the algorithms react rapidly to changes in the upstream produced water characteristics, at the same time as they reduce (or eliminate) steady-state oscillations. Based on both simulation and experimental testing, the study discusses advantages and disadvantages of the algorithms.
Humoral Mechanisms in T cell Vaccination: Induction and Functional Characterization of Anti-lymphocytic Autoantibodies
1997
T cell vaccination, the application of syngeneic attenuated T cells, has been shown to prevent effectively and treat experimental autoimmune diseases, but its mechanisms of action are poorly understood. Here we present data on the induction of a humoral anti-T cell response by T cell vaccination, capable of strongly inhibiting T cell proliferation and of ameliorating experimental autoimmune disease. T cell vaccination in the Lewis rat induced autoantibodies reactive with several syngeneic T cell proteins. These autoantibodies were not detectable in normal Lewis sera as assessed by immunoblotting and flow cytometry with intact syngeneic T cells. The autoantibody reactivity was not restricted…