Search results for "Clones"

Effect of deltamethrin (pyrethroid insecticide) on two clones of Daphnia magna (Crustacea, Cladocera): A proteomic investigation.

2014

8 pages; International audience; Deltamethrin is a class II pyrethroid insecticide commonly used in agriculture. It is hazardous to freshwater ecosystems, especially for the cladoceran Daphnia magna (Straus 1820). The results of our previous studies based on acute and chronic ecotoxicity experiments revealed differences in the sensitivity between two different clones. In this work, to investigate deltamethrin toxicity mechanisms in two clones of D. magna, we used a proteomic approach in order to analyze changes in protein expression profiles after 48h of exposure. We detected 1339 spots; then applying statistical criteria (ANOVA p<0.001 and minimum fold change 1.5), only 128 spots were sign…

Hydraulic kinetics of the graft union in different Olea europaea L. scion/rootstock combinations

2007

The hydraulic resistance of young olive trees grafted on rootstocks with contrasting size-controlling potential was measured 30, 90, 360 and 480 days after grafting. Olive (Olea europaea L.) clones inducing plant vigorous growth (Leccino ‘Minerva’, LM) or dwarfing (Leccino ‘Dwarf’, LD) were studied in different scion/rootstock combinations (LD/LD, LD/LM, LM/LD, LM/LM). Plants growing on LD root systems developed lower leaf surface areas (about 50% less) than plants grafted on LM rootstocks. Graft union represented the largest fraction (up to 85%) of whole-plant hydraulic resistance 30 days after grafting, and still represented an important fraction of it 2 months later (40–55%). Four-hundre…

Isolation of cell clones from stem cell population more resistant to oxidative stress for tissue repair.

2012

Cell clones more resistant to oxidative stress selecte from embryonic and adult mesoangioblast stem cell population.

2012

Distinct Clones of Yersinia pestis Caused the Black Death

2010

From AD 1347 to AD 1353, the Black Death killed tens of millions of people in Europe, leaving misery and devastation in its wake, with successive epidemics ravaging the continent until the 18th century. The etiology of this disease has remained highly controversial, ranging from claims based on genetics and the historical descriptions of symptoms that it was caused by Yersinia pestis to conclusions that it must have been caused by other pathogens. It has also been disputed whether plague had the same etiology in northern and southern Europe. Here we identified DNA and protein signatures specific for Y. pestis in human skeletons from mass graves in northern, central and southern Europe that …

Intratumoral Heterogeneity, Its Contribution to Therapy Resistance and Methodological Caveats to Assessment

2014

Cancer is one of the most urgent health issues of today. According to WHO, the number of cancer cases is expected to increase by 75% in the next two decades (1). Despite some remarkable achievements in the fields of cancer prevention and early detection, the goal of developing effective anti-cancer therapies still remains unmet. Tumor recurrence due to treatment resistance is the most common cause of death from cancer. Delineating cellular and molecular mechanisms underlying tumor recurrence is of prime importance for the ability to improve the efficacy of existing therapies and develop new strategies to cancer treatment.

Caracterización y tipificación molecular de cepas de Escherichia coli y Klebsiella pneumoniae productoras de betalactamasas de espectro extendido (BL…

2021

Introducción: Las betalactamasas de espectro extendido (BLEE) son enzimas producidas principalmente por la familia Enterobacteriaceae y se caracterizan porque inactivan a la práctica totalidad de los antibióticos betalactámicos. El aislamiento clínico de cepas productoras de BLEE es cada vez más frecuente en todo el mundo. Diferentes especies de Enterobacterales pueden ser productoras de BLEE, pero Klebsiella pneumoniae y Escherichia coli son las especies que las producen mayoritariamente. La realización de estudios de caracterización epidemiológica de las cepas productoras de BLEE es fundamental para detectar cualquier cambio en la resistencia y por tanto, para el control de su diseminació…

Responsible cognitive digital clones as decision-makers: A design science research study

2022

This study uses a design science research methodology to develop and evaluate the Pi-Mind agent, an information technology artefact that acts as a responsible, resilient, ubiquitous cognitive clone – or a digital copy – and an autonomous representative of a human decision-maker. Pi-Mind agents can learn the decision-making capabilities of their “donors” in a specific training environment based on generative adversarial networks. A trained clone can be used by a decision-maker as an additional resource for one’s own cognitive enhancement, as an autonomous representative, or even as a replacement when appropriate. The assumption regarding this approach is as follows: when someone was forced t…

Analysis of Multilocus Sequence Typing and Virulence Characterization of Listeria monocytogenes Isolates from Chinese Retail Ready-to-Eat Food

2016

Eighty Listeria monocytogenes isolates were obtained from Chinese retail ready-to-eat (RTE) food and were previously characterized with serotyping and antibiotic susceptibility tests. The aim of this study was to characterize the subtype and virulence potential of these L. monocytogenes isolates by multilocus sequence typing (MLST), virulence-associate genes, epidemic clones (ECs), and sequence analysis of the important virulence factor: internalin A (inlA). The result of MLST revealed that these L. monocytogenes isolates belonged to 14 different sequence types (STs). With the exception of four new STs (ST804, ST805, ST806, and ST807), all other STs observed in this study have been associat…

Genetic diversity of a clonal earthworm : gene expression variation and impacts on decomposition in metal contaminated soil

2016

Biodiversity affects ecosystem functioning positively. Metal-contamination diminishes species diversity and ecosystem function depends on the remaining species, and the genotypes within them. I studied how the clonally reproducing earthworm Dendrobaena octaedra responds to soil metal contamination and how its genetic diversity affects decomposition. Expression of the gene for the metal tolerance protein metallothionein was compared between populations with or without metal exposure history: with exposure history expression was high and stable and without there was a slow response. Adaptation to metal contamination likely explains the differences. Clonality of D. octaedra was verified with m…