Search results for "Colore"
showing 10 items of 1250 documents
Dissecting the Mechanism of Action of Spiperone—A Candidate for Drug Repurposing for Colorectal Cancer
2022
Approximately 50% of colorectal cancer (CRC) patients still die from recurrence and metastatic disease, highlighting the need for novel therapeutic strategies. Drug repurposing is attracting increasing attention because, compared to traditional de novo drug discovery processes, it may reduce drug development periods and costs. Epidemiological and preclinical evidence support the antitumor activity of antipsychotic drugs. Herein, we dissect the mechanism of action of the typical antipsychotic spiperone in CRC. Spiperone can reduce the clonogenic potential of stem-like CRC cells (CRC-SCs) and induce cell cycle arrest and apoptosis, in both differentiated and CRC-SCs, at clinically relevant co…
Expresión de microRNAs en la zonas de transición epitelio mesenquimal en el carcinoma colorectal como factor inmunomodulador y pronóstico. Estudio cl…
2018
El subgrupo mesenquimal (CMS4) de la clasificación molecular de consenso de cáncer colorectal es el tipo más agresivo y de peor pronóstico, y se caracteriza por la sobre expresión de genes implicados en el mecanismo de transición epitelio mesenquimal (TEM). La desdiferenciación tumoral focal (DTF) es el reflejo histológico del proceso de TEM. En base a estos conocimientos nos planteamos explorar la influencia de la DTF en la expresión de genes y microRNAs implicados en el proceso de TEM y evaluar su utilidad como factores predictivos de supervivencia. Analizamos de forma retrospectiva el grado de DTF en una serie de 125 especímenes de colectomía en todos los estadios clínicos. Evaluamos la …
Effetti di mutazioni diverse di ras in cellule di carcinoma colorettale (HT29)
2011
Cetuximab +/- chemotherapy enhances dendritic cell-mediated phagocytosis of colon cancer cells and ignites a highly efficient colon cancer antigen-sp…
2012
Cetuximab is a human/mouse chimeric IgG1 monoclonal antibody (mAb) to epidermal growth factor receptor, approved for colorectal carcinoma treatment in combination with chemotherapy. The immune-mediated effects elicited by its human fraction of crystallization moiety might critically contribute to the overall anti-tumor effectiveness of the antibody. We therefore investigated cetuximab ability to promote colon cancer cell opsonization and phagocytosis by human dendritic cells (DCs) that are subsequently engaged in antigen-cross presentation to cytotoxic T-lymphocyte (CTL) precursors. Human colon cancer cell lines were evaluated for susceptibility to DC-mediated phagocytosis before and after …
Bleaching of color of kraft pulp mill effluents and natural organic matter in lakes
2002
In situ changes in the color of lake water and biologically treated kraft pulp mill effluents mixed in lake water were examined in three lakes. In 1.7 m3 enclosures, the color (400700 g Pt·m3) of the mixture of pulp mill effluent and lake water remained similar for 4 months in darkness, whereas exposure to solar radiation resulted in 1742% bleaching of color. During the summer stratification of a humic lake, the color (160 g Pt·m3) of hypolimnetic water remained similar to that found during spring turnover, but the color of epilimnion decreased 13%. The measured rates of photochemical bleaching of color by solar radiation could explain the decrease of epilimnetic color. Attenuation of …
Omega-3 PUFAs and gut microbiota: A preventive approach for colorectal cancer
2021
The influence of diet on the composition of the intestinal microbiota and related pathologies has been known for some time. Some classes of nutrients, such as fatty acids belonging to the omega 3 series, have particular effects on the bacteria that make up the intestinal microbiota. ω-3 PUFAs affect the gut microbiota in three different ways: by modulating the type and abundance of intestinal bacteria, by regulate SCFAs levels, and by alter the levels of proinflammatory mediators. Through these modalities, ω-3 PUFAs could be useful for the prevention of intestinal diseases such as Colorectal Cancer (CRC). The ability of ω-3 PUFAs to modulate the intestinal inflammatory response, to preserve…
Nuclear protein phosphatase Wip1 regulate sensitivity of human colorectal cancer cells to DNA damaging anti-cancer agents
2019
Role of epigenetic factors in the selection of the alternative splicing isoforms of human
2017
Mutation-driven activation of KRAS is crucial to cancer development. The human gene yields four mRNA splicing isoforms, 4A and 4B being translated to protein. Their different properties and oncogenic potential have been studied, but the mechanisms deciding the ratio 4A/4B are not known. To address this issue, the expression of the four KRAS isoforms was determined in 9 human colorectal cancer cell lines. HCT116 and SW48 were further selected because they present the highest difference in the ratio 4A/4B (twice as much in HCT116 than in SW48). Chromatin structure was analysed at the exon 4A, characteristic of isoform 4A, at its intronic borders and at the two flanking exons. The low nucleoso…
Malignant colorectal obstruction. Palliative endoscopic treatment with self-expanding metallic stents
2005
The Authors report their experience in the palliative management of malignant colorectal obstruction. Between June 2000 and April 2003 sixteen patients (11 men and 5 women, age range 63-82) were found suffering from malignant colorectal obstruction. In these patients it was not possible to perform definitive surgical treatment because of the old age, advanced neoplastic disease and poor health condition, colostomy was the only therapeutic option. Endoscopic self-expanding stents placement was performed as a definitive palliative method to treat colorectal obstructions caused by advanced and unresectable tumours. This procedure was performed under endoscopic and fluoroscopic control and neit…
Developing liver spheroids for elucidating the role of colorectal cancer-derived smallextracellular vesicles in the pre-metastatic niche formation
2022
Introduction: Colorectal cancer (CRC) is the third most common tumor in the world frequently associated with liver metastasiscausing unfavorable prognosis. A recent study performed in our laboratory has demonstrated that CRC small extracellular vesi-cles (SEVs) induce an epithelial to mesenchymal transition (EMT) of hepatocytes (heps) driving them to actively participate inthe pre-metastatic niche formation, probably contributing to form a liver fibrotic microenvironment. Since 2D cell cultures par-tially reflect the structural complexity of the in vivo microenvironment to give more power to our functional model, we switchedto use hepatocyte spheroids (HeSPHs), which can give us more proper…