Search results for "Column chromatography"

showing 3 items of 143 documents

Platelet membrane fluidity, platelet membrane lipid pattern and platelet cytosolic Ca2+ content in subjects with vascular atherosclerotic disease

1994

In a group of subjects with vascular atherosclerotic disease (V AD) we examined the platelet membrane fluidity (obtained marking intact resting platelets with TMA-DPH), the platelet membrane cholesteroVphospholipid ratio (CIPL using column chromatography), the platelet membrane individual phospholipids (employing the thin layer chromatography) and the platelet cytosolic Ca2+ content (evaluated marking intact resting platelets with Fura 2-AM). From the obtained data, it is evident that platelet membrane fluidity differentiates normals from V AD subjects. Platelet membrane lipid pattern (CIPL and individual phospholipids) and cytosolic Ca2+ content do not discriminate normals from V AD subjec…

medicine.medical_specialtyPhysiologyVascular diseaseHematologyPhosphatidylserineBiologymedicine.diseasePLATELET MEMBRANE FLUIDITYCytosolchemistry.chemical_compoundEndocrinologyColumn chromatographyMembranechemistryPhysiology (medical)PhosphatidylcholineInternal medicinemedicinelipids (amino acids peptides and proteins)PlateletCardiology and Cardiovascular MedicineClinical Hemorheology and Microcirculation
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Characterization of the synthetic cannabinoid MDMB-CHMCZCA

2016

The synthetic cannabinoid MDMB-CHMCZCA was characterized by various spectroscopic techniques including NMR spectroscopy and tandem mass spectrometry. The synthetic sample was found to be of S-configuration by VCD spectroscopy and comparison of the data with DFT calculations, while ECD spectroscopy was found to be inconclusive in this case. The enantiomeric purity of samples from test purchases and police seizures was assessed by a self-developed chiral HPLC method.

medicine.medical_treatmentNPS010402 general chemistryTandem mass spectrometry01 natural sciencesFull Research Paperlcsh:QD241-441lcsh:Organic chemistrymedicinelcsh:Sciencechiral HPLCSpectroscopyChromatographyChemistry010401 analytical chemistryOrganic ChemistryNuclear magnetic resonance spectroscopy0104 chemical sciencesCharacterization (materials science)Chiral column chromatographyChemistryVCD spectroscopyECD spectroscopylcsh:QCannabinoidEnantiomersynthetic cannabinoidsBeilstein Journal of Organic Chemistry
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High-performance liquid chromatographic determination of diuretics in urine by micellar liquid chromatography.

1992

The use of micellar liquid chromatography for the determination of diuretics in urine by direct injection of the sample into the chromatographic system is discussed. The retention of the urine matrix at the beginning of the chromatograms was observed for different sodium dodecyl sulphate (SDS) mobile phases. The eluent strengths of a hybrid SDS-methanol micellar mobile phase for several diuretics were compared and related to the stationary phase/water partition coefficient with a purely micellar mobile phase. The urine band was appreciably narrower with a mobile phase of 0.05 M SDS-5% methanol (v/v) at 50 degrees C (pH 6.9). With this mobile phase the determination of bendroflumethiazide an…

medicine.medical_treatmentUrineHigh-performance liquid chromatographyMatrix (chemical analysis)Column chromatographyHydrochlorothiazideFurosemidemedicineHumansDiureticsChromatography High Pressure LiquidMicellesTriamtereneChromatographyChemistryProbenecidSodium Dodecyl SulfateGeneral ChemistryChlorothiazideAcetazolamideEthacrynic AcidHydrochlorothiazideMicellar liquid chromatographyBendroflumethiazideDiureticmedicine.drugChromatography LiquidJournal of chromatography
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