Search results for "Complex Mixture"
showing 10 items of 826 documents
Quantification of metal-resistant genes from the microbial community of uranium mine soils
2019
Background: Bacteria can thrive in environments presenting unique, extreme and/or stressful conditions, partly due to the diverse resistome they evolved as a result of physiological, metabolic and genetic adaptations. In this context, the microbial communities in soils from abandoned uranium mines may have peculiar genetic profiles that sustain valuable metal-resistance mechanisms. Getting further knowledge on the genetic potential of these microbial communities can be a contribute for developing bioremediation strategies towards the reclamation of highly contaminated uranium mine soils. Objectives: To quantify metal-resistance genes harboured by the microbial community of soil samples coll…
Characterization of the biosynthesis of saponins during seed development in peas (Pisum sativum)
2019
National audience; The use of pulses as ingredients for the production of food products rich in plant proteins is increasing. However, protein fractions prepared from pea or other pulses contain significant amounts of saponins, glycosylated triterpenes which can impart a bitter taste to the final food product. Bitter flavours are currently either removed by energy-requiring physico-chemical treatments or masked by additives. We are in the process of identifying and characterizing the genes involved in saponin biosynthesis during pea seed development, with the objective of identifying mutants in which seed saponins no longer accumulate. To do this we have applied a saponin extraction protoco…
Understanding the microbiological and physicochemical influence of alperujo vermicompost on diuron behaviour and soil quality
2009
Vermicompost as soil organic amendment increase the yield crops because improve the soil structure, the water retention capacity, the microbial biodiversity and thereby its metabolic activity. Vermicompost is also thought to reduce the pesticide pollution. The vermicompost selected was composed of “alperujo” which is largest organic residue in olive oil production. Agricultural soil was 5% amended twice and incubated for 3 months. A 3µg g-1 agricultural dosage of Diuron was applied to amended and unamended soils. The effect of alperujo vermicompost on dehydrogenase activity, the biodiversity and estimated bacterial density, the density of protocathechuate-degrading bacterial community and d…
Immobilization of murine noroviruses in a cultivated phaeozem soil and its reversibility
2017
Immobilization of murine noroviruses in a cultivated phaeozem soil and its reversibility. Environmental Microbiology & Microbial Ecology
Eradication of Plasmodiophora brassicae during composting of wastes
2006
Survival of infectious inoculum of the clubroot pathogen Plasmodiophora brassicae was assessed following bench-scale flask composting experiments and large-scale composting procedures. Clubroot-affected material was provided by artificial inoculation of Chinese cabbage or naturally infected Brussels sprout and cabbage roots. Both sets of diseased material were used in flask experiments, and the latter in large-scale windrow and aerated tunnel experiments. Municipal green wastes, onion waste and spent mushroom compost were evaluated in flask experiments with varying temperature, aeration and moisture conditions. Green wastes were used in larger-scale composts. Within the limits of a Chinese …
Aerobic composting and anaerobic digestion of pulp and paper mill sludges
1997
The feasibility of aerobic vessel composting and anaerobic digestion for the treatment of pulp and paper mill sludges were studied. The composting studies made use of primary and secondary sludge from a de-inking and paper mill. In six parallely aerated 500 1 vessels with various carbon : nitrogen (C:N) -ratios, the most optimal performance was obtained with C:N -ratios of c. 22–35, while higher and lower ratios delayed the temperature increase. With the optimal ratios, the thermophilic stage was reached within 36 h, and the stage lasted for about seven days. In the scale-up study (18 m3 compost vessel), the thermophilic stage was reached within 24 h. An effective dehydratation of the mass …
Structural Insight into CVB3-VLP Non-Adjuvanted Vaccine
2020
Coxsackievirus B (CVB) enteroviruses are common pathogens that can cause acute and chronic myocarditis, dilated cardiomyopathy, aseptic meningitis, and they are hypothesized to be a causal factor in type 1 diabetes. The licensed enterovirus vaccines and those currently in clinical development are traditional inactivated or live attenuated vaccines. Even though these vaccines work well in the prevention of enterovirus diseases, new vaccine technologies, like virus-like particles (VLPs), can offer important advantages in the manufacturing and epitope engineering. We have previously produced VLPs for CVB3 and CVB1 in insect cells. Here, we describe the production of CVB3-VLPs with enhanced pro…
Hsp60, a Novel Target for Antitumor Therapy: Structure-Function Features and Prospective Drugs Design
2013
Heat shock protein 60 kDa (Hsp60) is a chaperone classically believed to be involved in assisting the correct folding of other mitochondrial proteins. Hsp60 also plays a role in cytoprotection against cell stressors, displaying for example, antiapoptotic potential. Despite the plethora of studies devoted to the mechanism of Hsp60's function, especially in prokaryotes, fundamental issues still remain unexplored, including the definition of its role in cancer. Key questions still unanswered pertain to the differences in structure-function features that might exist between the well-studied prokaryotic GroEL and the largely unexplored eukaryotic Hsp60 proteins. In this article we discuss these …
Keyhole Limpet Haemocyanin in Experimental Bladder Cancer
2000
Objectives: Keyhole limpet haemocyanin (KLH) is a high-molecular-weight protein antigen collected from the haemolymph of the sea mollusk Megathura crenulata. It i
Chaperonopathies and chaperonotherapy. Hsp60 as therapeutic target in cancer: potential benefits and risks.
2013
In this minireview we focus on Hsp60 as a target for anticancer therapy. We discuss the new concepts of chaperonopathies and chaperonotherapy and present information on Hsp60 localization in the cell membrane of human tumor cells. We describe novel mechanisms for Hsp60 reaching the extracellular environment that involve membrane-associated stages, as well as data on anti-Hsp60 antibodies found in human sera, both in normal subjects and patients affected by autoimmune diseases. Finally, we discuss possible therapeutic applications of anti-Hsp60 antibodies in cancer treatment, evaluating also side effects on non-tumor cells. In conclusion, the way for investigating Hsp60-targeted anti-tumor t…