Search results for "Computer science"

A Robust and All-Inclusive Pipeline for Shuffling of Adeno-Associated Viruses.

2018

Adeno-associated viruses (AAV) are attractive templates for engineering of synthetic gene delivery vectors. A particularly powerful technology for breeding of novel vectors with improved properties is DNA family shuffling, i.e., generation of chimeric capsids by homology-driven DNA recombination. Here, to make AAV DNA shuffling available to a wider community, we present a robust experimental and bioinformatical pipeline comprising: (i) standardized and partially codon-optimized plasmids carrying 12 different AAV capsid genes; (ii) a scalable protocol including troubleshooting guide for viral library production; and (iii) the freely available software SALANTO for comprehensive analysis of ch…

A nondestructive intelligent approach to real‐time evaluation of chicken meat freshness based on computer vision technique

2019

In this study, the capability of a procedure based on combination of computer vision (CV) and artificial intelligence techniques examined for intelligent and nondestructive prediction of chicken meat freshness during the spoilage process at 4°C. The proposed system comprises the following stages: capture images, image preprocessing, image processing, computing channels, feature extraction, feature selection by a hybrid of genetic algorithm (GA) and artificial neuronal network (ANN), and prediction by using ANN. The number of neurons in input layer was determined 33 (selected features) and freshness used as the output. The ideal ANN model was obtained with 33‐10‐1 topology. The high performa…

Cost-efficiency assessments of marine monitoring methods lack rigor—a systematic mapping of literature and an end-user view on optimal cost-efficienc…

2021

Global deterioration of marine ecosystems, together with increasing pressure to use them, has created a demand for new, more efficient and cost-efficient monitoring tools that enable assessing changes in the status of marine ecosystems. However, demonstrating the cost-efficiency of a monitoring method is not straightforward as there are no generally applicable guidelines. Our study provides a systematic literature mapping of methods and criteria that have been proposed or used since the year 2000 to evaluate the cost-efficiency of marine monitoring methods. We aimed to investigate these methods but discovered that examples of actual cost-efficiency assessments in literature were rare, contr…

The application of the CRISPR-Cas9 genome editing machinery in food and agricultural science: Current status, future perspectives, and associated cha…

2019

The recent progress in genetic engineering has brought multiple benefits to the food and agricultural industry by enhancing the essential characteristics of agronomic traits. Powerful tools in the field of genome editing, such as siRNA-mediated RNA interference for targeted suppression of gene expression and transcription activator-like effector nucleases (TALENs) and zinc-finger nucleases (ZFNs) for DNA repair have been widely used for commercial purposes. However, in the last few years, the discovery of the CRISPR-Cas9 system has revolutionized genome editing and has attracted attention as a powerful tool for several industrial applications. Herein, we review current progresses in the uti…

Cross inhibition improves activity selection when switching incurs time costs

2015

Abstract We consider a behavioural model of an animal choosing between two activities, based on positive feedback, and examine the effect of introducing cross inhibition between the motivations for the two activities. While cross-inhibition has previously been included in models of decision making, the question of what benefit it may provide to an animal’s activity selection behaviour has not previously been studied. In neuroscience and in collective behaviour cross-inhibition, and other equivalent means of coupling evidence-accumulating pathways, have been shown to approximate statistically-optimal decision-making and to adaptively break deadlock, thereby improving decision performance. Sw…

Individual-Based Tracking Systems in Ornithology: Welcome to the Era of Big Data

2016

Technological innovations have led to exciting fast-moving developments in science. Today, we are living in a technology-driven era of biological discovery. Consequently, tracking technologies have facilitated dramatic advances in the fundamental understanding of ecology and animal behaviour. Major technological improvements, such as the development of GPS dataloggers, geolocators and other bio-logging technologies, provide a volume of data that were hitherto unconceivable. Hence we can claim that ornithology has entered the era of big data. In this paper, which is particularly addressed to undergraduate students and starting researchers in the emerging field of movement ecology, I summaris…

Decision support systems (DSS) for wastewater treatment plants - A review of the state of the art.

2019

The use of decision support systems (DSS) allows integrating all the issues related with sustainable development in view of providing a useful support to solve multi-scenario problems. In this work an extensive review on the DSSs applied to wastewater treatment plants (WWTPs) is presented. The main aim of the work is to provide an updated compendium on DSSs in view of supporting researchers and engineers on the selection of the most suitable method to address their management/operation/design problems. Results showed that DSSs were mostly used as a comprehensive tool that is capable of integrating several data and a multi-criteria perspective in order to provide more reliable results. Only …

On the Role of Perception: Understanding Stakeholders’ Collaboration in Natural Resources Management through the Evolutionary Theory of Innovation

2021

Natural resources management deals with highly complex socioecological systems. This complexity raises a conundrum, since wide-ranging knowledge from different sources and types is needed, but at the same time none of these types of knowledge is able by itself to provide the basis for a viable productive system, and mismatches between the two of them are common. Therefore, a growing body of literature has examined the integration of different types of knowledge in fisheries management. In this paper, we aim to contribute to this ongoing debate by integrating the evolutionary theory of innovation—and specifically the concept of proximity—and the theory of perception. We set up a theoretical …

Persistence of submerged macrophytes in a drying world: Unravelling the timing and the environmental drivers to produce drought-resistant propagules

2018

Discard ban: A simulation-based approach combining hierarchical Bayesian and food web spatial models

2020

12 pages, 6 figures, 6 tables, 2 appendixes, supplementary data https://doi.org/10.1016/j.marpol.2019.103703