Search results for "Confocal"

Confocal laser endomicroscopy for gastrointestinal diseases.

2008

Confocal laser endomicroscopy enables in vivo microscopy of the mucosal layer of the gastrointestinal tract with subcellular resolution during ongoing endoscopy. Endomicroscopy opens the door to immediate tissue and vessel analysis. Different types of diseases can be diagnosed with optical surface and subsurface analysis. Analysis of the in vivo microarchitecture can be used for targeting biopsies to relevant areas, and subsurface imaging can unmask microscopic diseases or bacterial infection. Molecular imaging is becoming feasible, which will enable new indications in gastrointestinal endoscopy. This article reviews the current and rapidly expanding clinical data on endomicroscopy and give…

Confocal laser endomicroscopy: technical status and current indications.

2006

Confocal laser endomicroscopy is a newly introduced endoscopic tool that makes it possible to carry out confocal microscopic examination of the mucosal layer during ongoing endoscopy. Different types of tissue and diseases can be diagnosed immediately, facilitating early diagnosis of gastrointestinal cancer. Analysis of the in vivo microarchitecture is helpful in targeting biopsies to relevant areas. In addition, subsurface imaging can unmask microscopic diseases - (microscopic colitis) or bacterial infection ( HELICOBACTER PYLORI), for example. Molecular imaging is becoming feasible, and this will shortly open the door to new indications in gastrointestinal endoscopy (e.g., in vivo recepto…

Sa1611 Computer-Assisted Diagnosis of Confocal Laser Endomicroscopy (CLE) by a Novel Image Analysis and Semantic Annotation Method

2012

Tu1656 Endomicroscopy of Intramuscular Neuronal Network Using Confocal Laser Endomicroscopy: A New Diagnostic Approach for Enteral Motility Disorders

2012

Advances in confocal laser endomicroscopy for the diagnosis of gastrointestinal diseases

2009

Confocal laser endomicroscopy (CLE) is a novel technique enabling in vivo microscopy of the human gastrointestinal mucosa. Cellular details even below the tissue surface can be visualized at high resolution during ongoing endoscopy.This review summarizes the current clinical data on the use of CLE in different disease states and discusses a perspective for future clinical and scientific application of CLE.Review on published literature and meeting abstracts.Confocal laser endomicroscopy covers a growing field of indications in both upper and lower gastrointestinal endoscopy and beyond. It has been shown to reliably predict the presence of neoplastic lesions and inflammatory changes of the g…

1136 In Vivo Endoscope-Based Confocal Laser Endomicroscopy (eCLE) Improves Detection of Unlocalized Barrett's Esophagus-Related Neoplasia Over High R…

2012

Advanced imaging of the gastrointestinal tract: research vs. clinical tools?

2009

Diagnostic endoscopy has moved forward considerably in the recent years. Still, three major needs have to be satisfied: endoscopy should be able to detect a lesion, characterize the lesion, and then its nature should be confirmed. These steps should ideally translate into an immediate therapeutic decision.High definition endoscopy has optimized our endoscopic view onto the mucosa and can be combined with digital surface enhancement modalities. Chromoendoscopy still holds a place to detect especially flat lesions in high-risk patients such as ulcerative colitis. Digital chromoendoscopy techniques such as narrow band imaging, i-scan, or Fuji intelligent chromo endoscopy offer new possibilitie…

Chromoendoscopy: What Is Its True Value for Ulcerative Colitis Surveillance?

2010

Advanced imaging technologies are enabling targeted biopsies or endoscopic resections due to better visualization of the mucosal architecture. This new concept of ‘smart biopsies’ is in particular important for patients with higher risk for development cancer (e.g. ulcerative colitis) but can also be beneficial for screening purposes. This short review will focus on new imaging modalities like chromoendoscopy, digital chromoendoscopy, high-definition endoscopy and confocal laser endomicroscopy in the lower GI tract which will be crucial in the future to detect colorectal neoplasia earlier then before.

Subcellular localization of bacteriophage PRD1 proteins in Escherichia coli

2014

Bacteria possess an intricate internal organization resembling that of the eukaryotes. The complexity is especially prominent at the bacterial cell poles, which are also known to be the preferable sites for some bacteriophages to infect. Bacteriophage PRD1 is a well-known model serving as an ideal system to study structures and functions of icosahedral internal membrane-containing viruses. Our aim was to analyze the localization and interactions of individual PRD1 proteins in its native host Escherichia coli. This was accomplished by constructing a vector library for production of fluorescent fusion proteins. Analysis of solubility and multimericity of the fusion proteins, as well as their …

Raman investigation of single oxidized carbon nanotubes

2001

The oxidation process of single-walled carbon nanotubes via nitric acid treatment was followed by IR-, UV-Vis-NIR, and single bundle Raman spectroscopy. The introduction of functional, oxygen-containing groups is revealed by an additional absorption band at 1725 cm−1, characteristic of carbonyl stretch vibrations. No significant shift of the optical absorption bands could be detected after oxidation. The combination of atomic force microscopy and confocal scanning resonance-enhanced Raman microscopy was used to investigate thin bundles and, eventually, individual nanotubes in detail. These experiments enabled determination of the dependence of the Raman intensity of the G-line (around 1590 …