Search results for "Confocal"
showing 10 items of 444 documents
On the morphology of Anisakis pegreffii: a comparative analysis of three microscopic techniques used to build a new parasite atlas
2014
BACKGROUND: Human anisakidosis is a parasitic anthropozoonosis caused by larval nematodes of the family Anisakidae. Here, we report a detailed description of the morphology of Anisakis pegreffii third-stage larva performed using a conventional light and confocal microscopy, and scanning electron microscopy (SEM) that provide a basis for both phenotypic studies and genetic mutations. METHODS: The collected larvae from fish were morphologically identified as Anisakis larvae Type I, and they were characterized by PCR-RFLP to identify the Anisakis pegreffii specie. Using NC5/NC2 primers, ribosomal genomic regions ITS1, 5.8 SrRNA and ITS2 of DNA were amplified and PCR products were sequenced. Fi…
Influencia de la conicidad de la guttapercha en la adaptación a la dentina de los materiales endodóncicos de obturación = Influencia da conicidade da…
2010
Para determinar la posible influencia de la conicidad de la gutapercha en la adaptación de los materiales de obturación a las paredes dentinarias, se obturaron 60 conductos radiculares de dientes humanos mediante tres técnicas de obturación diferentes (condensación lateral, técnica mixta y técnica de la onda continua) utilizando gutapercha de diferentes conicidades; conicidad normalizada (0,02%) y conicidad aumentada (0,004%) formándose 6 grupos de estudio. de 10 especímenes cada uno. Grupo 1 conductos obturados mediante la técnica de la condensación lateral y gutapercha de conicidad normalizada, grupo 2 condensación lateral/conicidad aumentada, grupo 3 técnica mixta/ conicidad normalizada,…
Extraction et évaluation de caractéristiques adaptées pour la classification du Lentigo à partir d’images de Microscopie Confocale
2019
International audience; La détection de cancer de la peau est l’un des défis de ces dernières décennies. Par ailleurs, diverses techniques d’imagerie ont pour objectif d’aider à la reconnaissance de ces pathologies malignes en contexte clinique. La Microscopie Confocale par Réflectance est un exemple de technique d’imagerie adaptée à la détection de maladie de la peau sur laquelle nous nous basons pour la détection de Lentigo. Les travaux présentés dans cet article portent sur la classification de ces images en trois catégories : sain, bénin et malin. Dans ce but, nous proposons et évaluons deux méthodes d’extraction de caractéristiques basées sur les descripteurs d’Haralick pour l’une et s…
Protection of islets in culture by delivery of oxygen binding neuroglobin via protein transduction.
2005
Islet transplantation has become an accepted method to treat type 1 diabetes. To succeed and achieve normal levels of glucose in transplant recipients, the quality of the transplanted islets is of the utmost importance. Lack of oxygen during organ procurement, islet isolation, and subsequent culture triggers apoptosis or necrosis and loss of islet function, causing the yield and quality to diminish. A promising candidate for cytoprotection against oxygen deprivation is neuroglobin (Ngb). Ngb is a recently described member of globin family and is expressed in neurons, retina, and pancreatic islets. To overexpress this protein in the islets and study its ability to protect them, we utilized p…
Effect of oxidoreduction potential and of gas bubbling on rheological properties and microstructure of acid skim milk gels acidified with glucono-del…
2009
Milk oxidoreduction potential was modified using gases during the production of a model dairy product and its effect on gel setting was studied. Acidification by glucono-delta-lactone was used to examine the physicochemistry of gelation and to avoid variations due to microorganisms sensitive to oxidoreduction potential. Four conditions of oxidoreduction potential were applied to milk: milk was gassed with air, nongassed, gassed with N(2), or gassed with N(2)H(2). The rheological properties and microstructure of these gels were determined using viscoelasticimetry, measurement of whey separation, and confocal laser scanning microscopy. It appeared that a reducing environment led to less-aggre…
Porous Gelatin Hydrogels: 2. In Vitro Cell Interaction Study
2007
We report on the feasibility of applying porous gelatin hydrogels, prepared by a novel and controlled cryogenic treatment, as cell-interactive scaffolds for tissue engineering applications. Despite the large number of publications on gelatin as a biomaterial, a detailed study of screening a limited number of gelatin scaffolds for their interaction with a panel of human cells has, to the best of our knowledge, not yet been published. In the present work, we have evaluated two types of porous gelatin scaffolds that differ in their pore geometry and pore size. Type I hydrogels contained top-to-bottom transverse channels (i.e. cones) with a decreasing diameter from the top (330 microm) to the b…
Interactions of virus proteins within the host cell
2014
Hydrophobin (HFBI): a potential fusion partner for one-step purification of recombinant proteins from insect cells
2008
Hydrophobins play an important role in binding and assembly of fungal surface structures as well as in medium-air interactions. These, hydrophobic properties provide interesting possibilities when purification of macromolecules is concerned. In aqueous micellar two-phase systems, based on surfactants, the water soluble hydrophobins are concentrated inside micellar structures and, thus, distributed to defined aqueous phases. This, one-step purification is attractive particularly when large-scale production of recombinant proteins is concerned. In the present study the hydrophobin HFBI of Trichoderma reesei was expressed as an N-terminal fusion with chicken avidin in baculovirus infected inse…
Enhanced Permeability and Retention-like Extravasation of Nanoparticles from the Vasculature into Tuberculosis Granulomas in Zebrafish and Mouse Mode…
2018
The enhanced permeability and retention (EPR) effect is the only described mechanism enabling nanoparticles (NPs) flowing in blood to reach tumors by a passive targeting mechanism. Here, using the transparent zebrafish model infected with Mycobacterium marinum we show that an EPR-like process also occurs allowing different types of NPs to extravasate from the vasculature to reach granulomas that assemble during tuberculosis (TB) infection. PEGylated liposomes and other NP types cross endothelial barriers near infection sites within minutes after injection and accumulate close to granulomas. Although similar to 100 and 190 nm NPs concentrated most in granulomas, even similar to 700 nm liposo…