Search results for "Cryoultramicrotomy"

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Primary cultures of human synovial macrophages metabolize androgens.

1996

AdultMalePrimary (chemistry)business.industryGeneral NeuroscienceMacrophagesSynovial MembraneMiddle AgedGeneral Biochemistry Genetics and Molecular BiologyMicrobiologyArthritis RheumatoidHistory and Philosophy of ScienceReceptors AndrogenAndrogensMedicineHumansFemaleTestosteronebusinessCells CulturedCryoultramicrotomyAgedAnnals of the New York Academy of Sciences
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Isolation and subsequent analysis of murine lamina propria mononuclear cells from colonic tissue

2007

Studies on colonic cells in the lamina propria (LP) of mice are important for understanding the cellular and immune responses in the gut, especially in inflammatory bowel diseases (such as morbus crohn and colitis ulcerosa). This protocol details a method to isolate LP cells and characterize freshly isolated cells by quality control experiments to obtain cells that can be used for further investigations. After different steps of digestion of the tissue using collagenase, DNase and dispase, the resulting cells are purified using Percoll gradient. The success of the isolation can be analyzed by cell viability test (Trypan Blue exclusion test) and by flow cytometric analysis to assess apoptosi…

Lamina propriabiologyColonCell Culture Techniquesfood and beveragesCell SeparationTransfectionGeneral Biochemistry Genetics and Molecular BiologyCell biologyTissue Culture TechniquesMicemedicine.anatomical_structureImmune systemCell cultureDispasemedicinebiology.proteinAnimalsLymphocytesViability assayIntestinal MucosaAntibodyPercollCryoultramicrotomyNature Protocols
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