Search results for "Cryptogein"

Nitric oxide (NO) in plants, a cell signalling messenger involved in plant defense. The case study of the N. tabacum / cryptogein model

2013

Communication vers les professionnels SPE IPM CT non renseigné car non soutenu par INRA

Rôle de la Dynamique Membranaire dans la Mise en Place des Mécanismes de Défense chez le Tabac

2011

Cryptogein, a protein secreted by the oomycete Phytophthora cryptogea, induces defense mechanisms in tobacco. Several proteins involved in the associated signaling pathway were identified and localized on the plasma membrane (PM). A fraction resistant to solubilization by detergent named DIMs for Detergent Insoluble Membranes, enriched in sterols an sphigolipids had been isolated from tobacco PM. It was proved to contain proteins previously identified as actors of the signaling cascade triggered by cryptogein. In animal cells, the dynamic association of proteins to sterol and sphingolipid rich microdomains under the influence of a biological stimulus plays an essential role in the regulatio…

Cryptogein treatment stimulates clathrin-endocytosis correlated with ROS production in tobacco cells

2007

International audience; During plant pathogen interactions, specific molecules elicit defense response of plant cells. Cryptogein, an elicitin produced by the pathogenic fungus Phytophtora cryptogea, induces a hypersensitive response (HR) in tobacco plants. The mode of action for cryptogein starts with recognition of this elicitor by an unidentified plasma membrane (PM) receptor. This ligand-receptor binding trigger a cascade of events at the PM level including rapid calcium influx, ions effluxes, extracellular medium alcalinization and plasma membrane depolarization both in tobacco plants and suspension cells. These events lead to the activation of plasma membrane-bound NADPH oxidase, Ntrb…

NO signaling in tobacco elicited by the MAMP cryptogein

2015

SPEPôle IPM; International audience; During the past years, nitric oxide (NO) has been shown to be a major cell signaling messenger in plants. Its importance has been highlighted during plant responses to pathogen attack or MAMPs (microbe associated molecular patterns) and during induced resistance or priming phenomenon. The major focus of our research is to understand how nitric oxide can modulate the activity of protein involved in plant defense. We identified several proteins undergoing S-nitrosylation, a redox-based post-translational modification of proteins, in tobacco cells elicited by cryptogein, a 10 kDa protein produced by the oomycete Phytophthora cryptogea and inducing immune re…

Calcium signaling in plant defense: involvement of subcellular compartments and glutamate receptorsCalcium signaling in plant defense: involvement of…

2012

NO signaling in cryptogein-induced immune responses in tobacco

2014

Study of the chaperone protein CDC48 and its involvement in plant immunity

2018

CDC48 is a conserved chaperone protein belonging to the AAA+ ATPase family (ATPase associated with various activities). This protein uses binding and hydrolysis of ATP to generate forces to affect the transformation of polypeptide substrate in numerous cellular processes. Studies on mammalian CDC48 orthologue revealed that it recognizes ubiquitylated polypeptides, directly or via partners, leading to substrate degradation or recycling. In plants, functions of CDC48 is less understood. The aim of my thesis, is to decipher the role of CDC48 in plant defense response context. First, I have to characterize NtCDC48 in tobacco (Nicotiana tabacum) suspension cells elicited by cryptogein, an elicit…

Effect of commercially available Plant Defence Stimulators on human innate immunity

2015

PDS (Plant defence stimulators) constitute a recent alternative to pesticides used for crop protection. These compounds are of diverse nature, they all act by stimulating plant innate immune system and plants can better fight pathogens. There are many similarities in pathogen perception systems and cellular signalling in plants and animals. Many elicitors stimulate both human and plant innate immunity (Zipfel and Felix, 2005). Therefore, it is likely that human innate immunity could be modulated by PDS. The aim of this study is to evaluate pro/anti-inflammatory activity of five different commercially available PDS on human cell models. We studied the pro/anti-inflammatory effect of PDS on h…

The role of NtRBOHD in regulation of response to cryptogein in tobacco cells

2014

International audience

Réponses des cellules de Nicotiana tabacum à des molécules microbiennes : évènements de signalisation précoce, influence de la dynamique membranaire …

2018

Responses of Nicotiana tabacum cells to microbial molecule treatments: early signaling events,influence of membrane dynamics, and sugar fluxesIn their natural environment plants are in close interaction with beneficial, neutral, or pathogenicmicrobes, which are highly dependent on carbon resources exuded by plant roots. Sugar transport, which isa key process of plant physiology, is essential to support the fate of plant-microbe interactions. Duringevolution, plants have acquired the ability to perceive microbial molecules, initiating specific signaltransduction cascades and leading to adapted response for microbe lifestyles (avirulent, virulent, or benefic).Plant survival will depend on the…