Search results for "Cth2"
showing 4 items of 4 documents
The role of post-transcriptional modulators of metalloproteins in response to metal deficiencies
2021
Copper and iron proteins play a wide range of functions in living organisms. Metal assembly into metalloproteins is a complex process, where mismetalation is detrimental and energy-consuming to cells. Under metal deficiency, metal distribution is expected to reach a metalation ranking, prioritizing essential versus dispensable metalloproteins, while avoiding interferences with other metals and protecting metal-sensitive processes. In this review, we propose that posttranscriptional Modulators of Metalloprotein messenger RNA (ModMeR) are good candidates in metal prioritization under metal-limited conditions. ModMeR target high quota or redundant metalloproteins and, by adjusting their synthe…
Translational repression and novel functions of Cth2 in the Saccharomyces cerevisiae response to iron deficiency
2020
El hierro (Fe) es un micronutriente y cofactor esencial para todos los organismos eucariotas. Las proteínas que incorporan hierro en su estructura lo hacen en forma de grupos hemo, centros Fe/S o centros de hierro y oxígeno, entre otros. Estos cofactores están implicados en numerosos procesos celulares como la respiración, la replicación y reparación del DNA, la biogénesis de ribosomas y la traducción de proteínas, la biosíntesis de ácidos nucleicos y lípidos, la fotosíntesis y el transporte de oxígeno. Pese a ser un metal abundante en la corteza terrestre, su forma oxidada Fe+3 es la más frecuente en un entorno oxidante y resulta insoluble a pH fisiológico. Esto hace de la deficiencia de h…
Molecular strategies to increase yeast iron accumulation and resistance.
2018
All eukaryotic organisms rely on iron as an essential micronutrient for life because it participates as a redox-active cofactor in multiple biological processes. However, excess iron can generate reactive oxygen species that damage cellular macromolecules. The low solubility of ferric iron at physiological conditions increases the prevalence of iron deficiency anemia. A common strategy to treat iron deficiency consists of dietary iron supplementation. The baker’s yeast Saccharomyces cerevisiae is used as a model eukaryotic organism, but also as a feed supplement. In response to iron deficiency, the yeast Aft1 transcription factor activates cellular iron acquisition. However, when constituti…
Post-Transcriptional Regulation of Iron Homeostasis in Saccharomyces cerevisiae
2013
Iron is an essential micronutrient for all eukaryotic organisms because it participates as a redox cofactor in a wide variety of biological processes. Recent studies in Saccharomyces cerevisiae have shown that in response to iron deficiency, an RNA-binding protein denoted Cth2 coordinates a global metabolic rearrangement that aims to optimize iron utilization. The Cth2 protein contains two Cx8Cx5Cx3H tandem zinc fingers (TZFs) that specifically bind to adenosine/uridine-rich elements within the 3' untranslated region of many mRNAs to promote their degradation. The Cth2 protein shuttles between the nucleus and the cytoplasm. Once inside the nucleus, Cth2 binds target mRNAs and stimulate…