Search results for "Culture"
showing 10 items of 11608 documents
Gametic and somatic embryogenesis through in vitro anther culture of different Citrus genotypes
2015
Abstract: In vitro tissue culture represents a useful technique for advancing Citrus breeding and propagation. Among in vitro regeneration systems, anther culture is commonly used to produce haploids and doubled haploids for a fast-track producing homozygous lines, in comparison with the traditional self-pollination approach, which involves several generations of selfing. In addition, anthers culture can produce somatic embryos that can also be used for clonal propagation. In this study, two thermal shocks were applied to the anthers of six Citrus genotypes (two clementine and four sweet oranges), just after they were put in culture. The response obtained was different depending on the geno…
Deregulated High Affinity Copper Transport Alters Iron Homeostasis inArabidopsis
2020
The present work describes the effects on iron homeostasis when copper transport was deregulated in Arabidopsis thaliana by overexpressing high affinity copper transporters COPT1 and COPT3 (COPTOE). A genome-wide analysis conducted on COPT1OE plants, highlighted that iron homeostasis gene expression was affected under both copper deficiency and excess. Among the altered genes were those encoding the iron uptake machinery and their transcriptional regulators. Subsequently, COPTOE seedlings contained less iron and were more sensitive than controls to iron deficiency. The deregulation of copper (I) uptake hindered the transcriptional activation of the subgroup Ib of basic helix-loop-helix (bHL…
Preliminary Study on the In vitro and In vivo Effects of Asparagopsis taxiformis Bioactive Phycoderivates on Teleosts
2016
Several compounds from marine organisms have been studied for their potential use in aquaculture. Among the red algae, Asparagopsis taxiformis is considered one of the most promising species for the production of bioactive metabolites with numerous proposed applications. Here, the in vitro antibacterial activity, the easy handling and the absence of adverse effects on marine fish species are reported. Depending on the seasonal period of sampling, ethanol extracts of A. taxiformis exhibited significantly different inhibitory activity against fish pathogenic bacteria. The extract obtained in late spring showed strong antibacterial activity against Aeromonas salmonicida subsp. salmonicida, Vib…
Clonal populations of Clavibacter michiganensis subsp michiganensis are responsible for the outbreaks of bacterial canker in greenhouse tomatoes in I…
2015
Clavibacter michiganensis subsp. michiganensis (Cmm) strains, collected in greenhouses from 17 farms during tomato bacterial canker outbreaks occurring between 2005 and 2008 in Sicily, were analysed by a multiphasic approach. Population studies were conducted to investigate the possible sources of inocula. Cmm strains were characterized by PCR assays targeting virulence genes, fingerprinting techniques, metabolic profiles and virulence. These strains were comparatively analysed with Cmm strains isolated in other parts of Italy over a period of 15 years. Chromosomal genes encoding virulence determinants tomA, ppaA, chpC, and the plasmid-encoded genes pat-1 and celA were detected by PCR in al…
Micropropagation of Sicilian cultivars with an aim to preserve genetic diversity in hazelnut (Corylus avellana L.)
2018
The use of a small number of cultivars in agriculture can lead to a loss of agrobiodiversity. Since in vitro techniques are valuable tools for conserving plant biodiversity, an efficient micropropagation protocol for four Italian hazelnut cultivars, ‘Carrello’, ‘Ghirara’, ‘Minnulara’, and ‘Panottara’, was developed. The highest axillary bud survival was obtained after decontamination with 40 min 1% sodium hypochlorite followed by 40 min 0.1% sodium merthiolate in ‘Minnulara’ and ‘Ghirara’, while the 35þ35 min treatment was the best for ‘Carrello’ and ‘Panottara’. Shoot multiplication was higher in ‘Minnulara’ and ‘Ghirara’ when 6.6 lM N6-benzyladenine was used, even if some hyperhydric shoo…
Strategies to Mitigate the Salt Stress Effects on Photosynthetic Apparatus and Productivity of Crop Plants
2018
Soil salinization represents one of the major limiting factors of future increase in crop production through the expansion or maintaining of cultivation area in the future. High salt levels in soils or irrigation water represent major environmental concerns for agriculture in semiarid and arid zones. Recent advances in research provide great opportunities to develop effective strategies to improve crop salt tolerance and yield in different environments affected by the soil salinity. It was clearly demonstrated that plants employ both the common adaptative responses and the specific reactions to salt stress. The review of research results presented here may be helpful to understand the physi…
Use of plant growth-promoting rhizobacteria (PGPR) and organic fertilization for soilless cultivation of basil
2021
Abstract Today there is a greater environmental and ecological awareness and it is growing the number of farmers who want to adopt sustainable and efficient cultivation systems even if not officially certified as organic. Sustainable and modern cultivation systems must involve organic fertilization and cannot ignore the role of rhizosphere microorganisms. Starting from this premise, this paper aimed to evaluate the use of plant growth-promoting rhizobacteria (PGPR) and organic liquid fertilizers on soilless cultivation of basil. Genovese basil plants were cultivated in pots filled with a substrate inoculated or not with a commercial biostimulant (TNC BactorrS13) containing growth-promoting …
A Model for ERD2 Function in Higher Plants
2020
ER lumenal proteins have a K(H)DEL motif at their C-terminus. This is recognized by the ERD2 receptor (KDEL receptor in animals), which localizes to the Golgi apparatus and serves to capture escaped ER lumenal proteins. ERD2-ligand complexes are then transported back to the ER via COPI coated vesicles. The neutral pH of the ER causes the ligands to dissociate with the receptor being returned to the Golgi. According to this generally accepted scenario, ERD2 cycles between the ER and the Golgi, although it has been found to have a predominant Golgi localization. In this short article, we present a model for the functioning of ERD2 receptors in higher plants that explains why it is difficult t…
Transcriptome approach to understand the potential mechanisms inhibiting or triggering blossom-end rot development in tomato fruit in response to pla…
2017
The objectives of this study were to analyze changes in gene expression and identify candidate genes and gene networks potentially inhibiting or triggering blossom-end rot (BER) in tomatoes treated with plant growth regulators. ?Ace 55 (Vf)? tomato plants were grown in a greenhouse and sprayed with Apogee (300 mg L?1), abscisic acid (ABA) (500 mg L?1), water (control), or gibberellins 4?+?7 (GA4?+?7) (300 mg L?1) weekly after pollination. The BER incidence rate was zero in Apogee- and ABA-, medium in water-, and high in GA4?+?7-treated plants from 26 to 40 days after pollination (DAP). At 26 DAP, healthy blossom-end fruit tissue still not showing visible BER symptoms was used for transcript…
Transcriptional responses to pre-flowering leaf defoliation in grapevine berry from different growing sites, years, and genotypes
2017
Leaf removal is a grapevine canopy management technique widely used to modify the sourceâsink balance and/or microclimate around berry clusters to optimize fruit composition. In general, the removal of basal leaves before flowering reduces fruit set, hence achieving looser clusters, and improves grape composition since yield is generally curtailed more than proportionally to leaf area itself. Albeit responses to this practice seem quite consistent, overall vine performance is affected by genotype, environmental conditions, and severity of treatment. The physiological responses of grape varieties to defoliation practices have been widely investigated, and just recently a whole genome trans…