Search results for "Cultures"

Analysis of MSCs' secretome and EVs cargo: Evaluation of functions and applications

2022

Mesenchymal stem cells (MSCs) can exert different functions and can be used in several medical fields. In the last years, MSC properties have been attributed to their secreted factors such as soluble proteins, cytokines and growth factors. Moreover, a key role is played by the extracellular vesicles (EVs) which lead a heterogeneous cargo of proteins, lipids and small and long non-coding RNAs that interfere with the pathways of the recipient cells. Due to the safeness and easiness in obtaining the secretome, its use is becoming a turning point for the application in physiological and pathological fields. This review summarizes the most recent studies on the use of MSCs secretome, focusing on…

Impact of Gluten-Friendly Bread on the Metabolism and Function of In Vitro Gut Microbiota in Healthy Human and Coeliac Subjects

2016

The main aim of this paper was to assess the in vitro response of healthy and coeliac human faecal microbiota to gluten-friendly bread (GFB). Thus, GFB and control bread (CB) were fermented with faecal microbiota in pH-controlled batch cultures. The effects on the major groups of microbiota were monitored over 48 h incubations by fluorescence in situ hybridisation. Short-chain fatty acids (SCFAs) were measured by high-performance liquid chromatography (HPLC). Furthermore, the death kinetics of Lactobacillus acidophilus, Bifidobacterium animalis subsp. lactis, Staphylococcus aureus, and Salmonella Typhimurium in a saline solution supplemented with GFB or CB were also assessed. The experiment…

Detection of bacteria and yeast species by the BACTEC 9120 automated system with the routine use of aerobic, anaerobic, and fungal media

2008

ABSTRACT During the period 2006 and 2007, all blood cultures required by four units at high infective risk and most of those required by other units of the University Hospital of Palermo, Palermo, Italy were performed using a Bactec 9120 automated blood culture system with a complete set of Plus Aerobic/F, Plus Anaerobic/F, and Mycosis IC/F bottles. The aim of the study was to enable the authors to gain firsthand experience of the culture potentialities of the three different media, to obtain information regarding the overall and specific recovery of bacteria and yeasts from blood cultures in the hospital, and to reach a decision as to whether and when to utilize anaerobic and fungal bottle…

Effect of sequential inoculum of beta-glucosidase positive and probiotic strains on brine fermentation to obtain low salt sicilian table olives

2019

In the present study, the β-glucosidase positive strain Lactobacillus plantarum F3. 3 was used as starter during the fermentation of Sicilian table olives (Nocellara Etnea cultivar) at two different salt concentrations (5 and 8%), in order to accelerate the debittering process. The latter was monitored through the increase of hydroxytyrosol compound. In addition, the potential probiotic Lactobacillus paracasei N24 strain was added after 60 days of fermentation. Un-inoculated brine samples at 5 and 8% of salt were used as control. The fermentation was monitored till 120 days through physico-chemical and microbiological analyses. In addition, volatile organic compounds and sensorial analyses …

Missense variants in DPYSL5 cause a neurodevelopmental disorder with corpus callosum agenesis and cerebellar abnormalities

2021

International audience; The collapsin response mediator protein (CRMP) family proteins are intracellular mediators of neurotrophic factors regulating neurite structure/spine formation and are essential for dendrite patterning and directional axonal pathfinding during brain developmental processes. Among this family, CRMP5/DPYSL5 plays a significant role in neuronal migration, axonal guidance, dendrite outgrowth, and synapse formation by interacting with microtubules. Here, we report the identification of missense mutations in DPYSL5 in nine individuals with brain malformations, including corpus callosum agenesis and/or posterior fossa abnormalities, associated with variable degrees of intel…

Influence of Laccase and Tyrosinase on the Antioxidant Capacity of Selected Phenolic Compounds on Human Cell Lines

2015

Polyphenolic compounds affect the color, odor and taste of numerous food products of plant origin. In addition to the visual and gustatory properties, they serve as radical scavengers and have antioxidant effects. Polyphenols, especially resveratrol in red wine, have gained increasing scientific and public interest due to their presumptive beneficial impact on human health. Enzymatic oxidation of phenolic compounds takes place under the influence of polyphenol oxidases (PPO), including tyrosinase and laccase. Several studies have demonstrated the radical scavenger effect of plants, food products and individual polyphenols in vitro, but, apart from resveratrol, such impact has not been prove…

Report on the first season of German-Kurdish excavations at Muqable in 2015

2017

In 2015, a new excavation project entitled “Kurdish-German Archaeological Mission in Dohuk” (KUGAMID) was launched. It is organized as a joint project between the University of Tübingen and the Department of Antiquities of Dohuk and is jointly directed by Peter Pfälzner (Tübingen) and Hassan Ahmad Qasim (Dohuk). Three sites were selected for excavation: Bassetki, Muqable I and Muqable III. The following report will summarize the results of the first season of excavations at the two neighbouring mounds of Muqable I and Muqable III, located approximately 5 km southeast of Bassetki and 23 km west of Dohuk in the Autonomous Region of Kurdistan in Iraq. The first season of excavations at Muqable…

Wordarrows: El poder representativo del lenguaje en la obra de no ficción de N. Scott Momaday

2012

This article focuses on two non-fiction works by Native American author N. Scott Momaday: his 1969 historical memoir The Way to Rainy Mountain and his essay collection The Man Made of Words. It specifically tackles performative conceptions of language in the Kiowa storytelling tradition, where words are experienced as speech acts that have the power to intervene in surrounding realities. Taking into account 20th century ethno-cultural and linguistic policies in the United States, the article also reflects on the role indigenous languages may play in contemporary Native American Literature, which has most often been written in English.

Führer durch die Ausstellung zur Erinnerung an den Vaterländischen Krieg von 1812: vom 17. November 1912 bis 6. Januar 1913

1912

Astronomiskās un dabas parādības aizvēsturē Ziemeļeiropā: atspoguļojums vēstures avotos un arheoloģiskajā materiālā

2021

Cilvēka dzīve aizvēsturē bija ļoti cieši saista ar dabu. Līdz ar pāreju uz ražotājsaimniecību vēlā akmens laikmeta beigās, bet īpaši sākot ar bronzas laikmetu, izveidojās ideoloģija, kurā centrālo vietu ieņēma Saule un ar to saistīto gadskārtu cikli. Solārā un lunārā simbolika plaši pārstāvēta Ziemeļeiropas, arī Latvijas arheoloģiskajā materiālā. Dabas un astronomiskās parādības minētas arī pirmajos rakstītajos avotos, bet to sekas – īpaši dabas katastrofu sekas, fiksējamas arī arheoloģiskajā materiālā.