Search results for "Cytochrome b"
showing 10 items of 63 documents
Climatic oscillations triggered post-Messinian speciation of Western Palearctic brown frogs (Amphibia, Anura, Ranidae)
2003
Abstract Oscillating glacial cycles over the past 2.4 million years are proposed to have had a major impact on the diversity of contemporary species communities. We used mitochondrial and nuclear DNA sequence data to infer phylogenetic relationships within Western Palearctic brown frogs and to test the influence of Pliocene and Pleistocene climatic changes on their evolution. We sequenced 1976 bp of the mitochondrial genes 16S rRNA and cytochrome b and of the nuclear rhodopsin gene for all current species and subspecies. Based on an established allozyme clock for Western Palearctic water frogs and substitution rate constancy among water frogs and brown frogs, we calibrated a molecular clock…
Two changes of the same nucleotide confer resistance to diuron and antimycin in the mitochondrial cytochrome b gene of Schizosaccharomyces pombe
1988
AbstractDiuron (3-(3,4-dichlorophenyl)-1,1-dimethylurea) and antimycin, both inhibitors of mitochondrial respiration, block electron flow between cytochromes b and c1. Mutants resistant to either drug have been selected using Schizosaccharomyces pombe strains with an extrachromosomally inherited mutator. In analogy to Saccharomyces cerevisiae these mutational sites were assumed to map in the cytochrome b gene. DNA sequence analysis showed that two changes in the same nucleotide are responsible for resistance to antimycin and diuron. Analysis of resistant and sensitive progeny of crosses between the mutants and the wild type confirmed the correlation between mutational alteration and resista…
Genetic population structure of Epinephelus marginatus (Pisces, Serranidae) revealed by two molecular markers
2006
In this paper, data are presented regarding the population structure of dusky grouper (Epinephelus marginatus) which was sampled in the Mediterranean Sea and Atlantic Ocean using two different molecular markers, ND2 RFLP and cytochrome b (cyt b) sequence analysis. The main objective of the study is to analyse the genetic variability of E. marginatus in the Mediterranean Sea to define how the biological characteristics of the species, such as the dispersal capability of pelagic larvae and the benthonic-sedentary life style, can affect the genetic population structure or maintaining the gene flow or determining genetic differences. Furthermore, we considered an Atlantic sample to establish th…
Using phylogeography to promote dormouse conservation: the case of Muscardinus avellanarius (Rodentia, Gliridae)
2012
this study describes the phylogeographic history of the common dormouse, Muscardinus avellanarius rodent strictly protected in europe (habitat directive, annex iv; Bern convention, annex iii). We analyzed the genetics of 120 common dormice across the species’ range, using sequences data from the mitochondrial cytochrome b gene (704 pb). the dataset obtained was analyzed using different phylogenetic reconstructions as well as other methods adapted to phylogeography. A complex phylogeographic pattern has been retrieved from the mitochondrial dnA gene, with the presence of two highly divergent lineages. these two lineages are themselves subdivided into five sublineages, which should be regarde…
New insights into the taxonomy and phylogeny of social voles inferred from mitochondrial cytochrome b sequences
2012
We sequenced the entire cytochrome b gene in Microtus paradoxus from Turkmenistan and Microtus socialis from Crimea and Kalmykia. Phylogenetic relationships among social voles were reconstructed by the inclusion into analyses of a further 23 published haplotypes belonging to six species. The two probabilistic methods which were used in phylogenetic analyses, the Bayesian inference and Maximum Likelihood, yielded very similar results. Both trees showed two highly divergent lineages which were further subdivided into seven species. The socialis lineage encompassed four species (M. socialis, M. irani, M. anatolicus, and M. paradoxus), and the remaining three species clustered into the guenther…
Membrane D-lactate oxidase in Zymomonas mobilis: evidence for a branched respiratory chain.
1998
Respiratory chain composition of the ethanol-producing bacterium Zymomonas mobilis was studied. Its membrane D-lactate oxidase was characterised. With NADH, but not D-lactate as substrate, a cytochrome o-like component was seen in CO difference spectra. Chlorpromazine specifically inhibited reduction of cytochrome d, while myxothiazol eliminated the cytochrome o-like features in CO difference spectra. It is suggested that electrons from NADH are distributed between branches terminated by the cytochrome o-like component, cytochrome a, and cytochrome d. With D-lactate, electrons are transported to cytochrome a, or an unidentified CN(-)-sensitive oxidase, and cytochrome d.
Phylogeny and species limits in the Palaearctic chiffchaffPhylloscopus collybitacomplex: mitochondrial genetic differentiation and bioacoustic eviden…
1996
Nucleotide sequences of the mitochondrial cytochrome b gene (1041 bp), analysis of vocalizations and behavioural evidence from zones of contact were used to reassess the species limits and phylogenetic relationships at the species and subspecies levels in the Phylloscopus collybita complex. A new classification is proposed which recognizes four biological species. Phylloscopus brehmii (Iberia) and P. canariensis (Canary Islands) are genetically and bioacoustically highly distinct. There is no mitochondrial gene flow between them or with P. collybita. The Mountain Chiffchaff P. sindianus (with subspecies sindianus and Iorenzii) is equally distinct genetically from southwest Asian subspecies …
Structure of the Zymomonas mobilis respiratory chain: oxygen affinity of electron transport and the role of cytochrome c peroxidase
2014
The genome of the ethanol-producing bacterium Zymomonas mobilis encodes a bd-type terminal oxidase, cytochrome bc 1 complex and several c-type cytochromes, yet lacks sequences homologous to any of the known bacterial cytochrome c oxidase genes. Recently, it was suggested that a putative respiratory cytochrome c peroxidase, receiving electrons from the cytochrome bc 1 complex via cytochrome c 552, might function as a peroxidase and/or an alternative oxidase. The present study was designed to test this hypothesis, by construction of a cytochrome c peroxidase mutant (Zm6-perC), and comparison of its properties with those of a mutant defective in the cytochrome b subunit of the bc 1 complex (Zm…
Siguiendo el ejemplo fenicio: colonización del Mediterráneo occidental por Spirobranchus cf. tetraceros (Annelida: Serpulidae)
2020
A newly established population of the fouling polychaete Spirobranchus cf. tetraceros is reported from the western Mediterranean (Valencia Port). Despite previous intensive surveys, this is the first record for the taxon in the Iberian Peninsula. Molecular analyses revealed that S. cf. tetraceros from Valencia are genetically identical to specimens from Heraklion, Crete, but different from those collected in the Red Sea and S. tetraceros sensu stricto from the type locality in Australia. Mediterranean and Red Sea S. cf. tetraceros form a well-supported monophyletic clade but are clearly distinct from New South Wales specimens of S. tetraceros. Our new molecular evidence supports the hypothe…
Phylogenetic relationships of the Italian populations of Horseshoe Whip Snake Hemorrhois hippocrepis (Serpentes, Colubridae)
2020
Hemorrhois hippocrepis is a colubrid snake with a West Mediterranean distribution. It is widespread in the Iberian Peninsula and Northwest Africa. The only Italian populations are found on the islands of Sardinia and Pantelleria. The phylogenetic relationships of these insular populations have been analysed for the first time on the basis of the mitochondrial DNA cytochrome b gene. The sequences were compared with those available from the geographic range of this species. The analyses showed that the Italian samples are part of a lineage that groups Tunisian and East Algerian samples, with which they share the same haplotype. These results strongly support the hypothesis of a recent origin …