Search results for "Cytomegalovirus infections"

showing 3 items of 153 documents

Need to evaluate the performance of real-time PCR assays for the quantitation of cytomegalovirus DNA load in lower respiratory tract specimens

2013

There is an increasing appreciation for the potential clinical value of the quantitation of cytomegalovirus (CMV) DNA in the lower respiratory tract in critically ill patients lacking canonical immunosuppression, in view of the possible pathogenic role of CMV in these patients [1]. No data have been published on the analytical performance of real-time PCR assays for this purpose. We present our data on the performance of the COBAS® AmpliPrep/COBAS® TaqMan CMV PCR Assay (Roche Diagnostics, Mannheim, Germany) for the quantitation of CMV DNA in tracheal aspirates (TA). This CMV PCR assay has been approved recently by the US Food and Drug Administration for use with plasma specimens [2]. We cho…

medicine.medical_specialtyLettermedicine.medical_treatmentCongenital cytomegalovirus infectionCytomegalovirusCritical Care and Intensive Care MedicineReal-Time Polymerase Chain ReactionGastroenterologySensitivity and SpecificityCytomegalovirus DNAInternal medicinemedicineTaqManHumansRespiratory Tract InfectionsMechanical ventilationRespiratory tract infectionsbusiness.industryViral Loadmedicine.diseaseVirologyRespiration ArtificialIntensive Care Unitsmedicine.anatomical_structureReal-time polymerase chain reactionCytomegalovirus InfectionsDNA ViralbusinessViral loadRespiratory tract
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Differences in cytomegalovirus plasma viral loads measured in allogeneic hematopoietic stem cell transplant recipients using two commercial real-time…

2010

5 páginas, 3 figuras.

medicine.medical_treatmentCongenital cytomegalovirus infectionCytomegalovirusHematopoietic stem cell transplantationHematopoietic stem cell transplantationBiologyPolymerase Chain ReactionAutomationPlasmaVirologymedicineHumansViral loadHematopoietic Stem Cell Transplantationvirus diseasesViral LoadUniversity hospitalmedicine.diseaseVirologyDNA extractionAllogeneic stem cell transplantReverse transcription polymerase chain reactionInfectious DiseasesReal-time polymerase chain reactionSpainImmunologyCytomegalovirus InfectionsAllogeneic hematopoietic stem cell transplantViral loadReal-time PCRJournal of clinical virology : the official publication of the Pan American Society for Clinical Virology
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Mouse models of cytomegalovirus latency: overview.

2002

Abstract Background: The molecular regulation of viral latency and reactivation is a central unsolved issue in the understanding of cytomegalovirus (CMV) biology. Like human CMV (hCMV), murine CMV (mCMV) can establish a latent infection in cells of the myeloid lineage. Since mCMV genome remains present in various organs after its clearance from hematopoietic cells first in bone marrow and much later in blood, there must exist one or more widely distributed cell type(s) representing the cellular site(s) of enduring mCMV latency in host tissues. Endothelial cells and histiocytes are candidates, but the question is not yet settled. Another long debated problem appears to be solved: mCMV establ…

virusesCytomegalovirusBiologymedicine.disease_causeVirusHerpesviridaeImmediate-Early ProteinsTransactivationMiceViral ProteinsVirologyVirus latencymedicineCytotoxic T cellAnimalsHumansLatency (engineering)GeneMice Inbred BALB Cvirus diseasesmedicine.diseaseVirologyVirus LatencyHaematopoiesisDisease Models AnimalInfectious DiseasesImmunologyCytomegalovirus InfectionsTrans-ActivatorsVirus ActivationJournal of clinical virology : the official publication of the Pan American Society for Clinical Virology
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