Search results for "D region"

Derepressing muscleblind expression by miRNA sponges ameliorates myotonic dystrophy-like phenotypes in Drosophila

2016

AbstractMyotonic Dystrophy type 1 (DM1) originates from alleles of the DMPK gene with hundreds of extra CTG repeats in the 3′ untranslated region (3′ UTR). CUG repeat RNAs accumulate in foci that sequester Muscleblind-like (MBNL) proteins away from their functional target transcripts. Endogenous upregulation of MBNL proteins is, thus, a potential therapeutic approach to DM1. Here we identify two miRNAs, dme-miR-277 and dme-miR-304, that differentially regulate muscleblind RNA isoforms in miRNA sensor constructs. We also show that their sequestration by sponge constructs derepresses endogenous muscleblind not only in a wild type background but also in a DM1 Drosophila model expressing non-co…

Molecular Effects of the CTG Repeats in Mutant Dystrophia Myotonica Protein Kinase Gene

2008

Myotonic Dystrophy type 1 (DM1) is a multi-system disorder characterized by muscle wasting, myotonia, cardiac conduction defects, cataracts, and neuropsychological dysfunction. DM1 is caused by expansion of a CTG repeat in the 3 untranslated region (UTR) of the Dystrophia Myotonica Protein Kinase (DMPK) gene. A body of work demonstrates that DMPK mRNAs containing abnormally expanded CUG repeats are toxic to several cell types. A core mechanism underlying symptoms of DM1 is that mutant DMPK RNA interferes with the developmentally regulated alternative splicing of defined pre-mRNAs. Expanded CUG repeats fold into ds(CUG) hairpins that sequester nuclear proteins including human Muscleblind-lik…

Lidocaine versus ropivacaine for postoperative continuous paravertebral nerve blocks in patients undergoing laparoscopic bowel surgery: a randomized,…

2015

Daniela Ghisi,1,3 Andrea Fanelli,1,2 Julie Jouguelet-Lacoste,1 Luca La Colla,1,4 Anne-Sophie Auroux,1,5 Jacques E Chelly1 1Department of Anesthesiology, University of Pittsburgh Medical Center, Pittsburgh, PA, USA; 2Anesthesia and Intensive Care Unit, Policlinico S.Orsola-Malpighi, Bologna, Italy; 3Department of Anesthesia and Postoperative Intensive Care, Istituto Ortopedico Rizzoli, Bologna, Italy; 4Department of Anesthesia, Intensive Care and Pain Therapy, Università degli Studi di Parma, Parma, Italy 5Institut des Sciences Pharmaceutiques et Biologiques-Faculté de Pharmacie de Lyon Université Claude Bernard Lyon, FranceBackground and objectives: Lidocain…

Towards the ‘rurban’ scenario: plural forms of planning in Europe for a new alliance between city and countryside - Verso lo scenario ‘rurban’: forme…

2015

Nell’ultimo decennio si è manifestata una crescente attenzione alle relazioni di interdipendenza tra di- mensione urbana e dimensione rurale dello sviluppo territoriale. È un interesse alimentato dalla diversa percezione con cui ormai viene colto il rapporto tra città e campagna, tra economie urbane ed economie rurali, considerate dal pensiero economico-politico classico come due mondi separati. Questo processo è particolarmente visibile nel con- testo europeo, dove per ragioni non solo insediative e funzionali, ma anche economiche, sociali e culturali l’avvento di modelli di sviluppo post-industriali ha portato a più complesse forme di ibridazione tra modelli di sviluppo urbani e rurali. L…

Il ri-ciclo socialmente innovativo come attivatore di sviluppo urbano e regionale. I brownfield come contesto di analisi e azione.

The research topic is the re-cycling of brownfields with social innovative practices, and how it can activate the urban and regional development. The research analyses critically and interprets the re-cycle theory (Marini and Bocchi 2014; Carta 2014) developed by the Italian Research “Re-cycle Italy” (PRIN) focusing on its social innovative aspects (Moulaert et al., 2005; Phills et al., 2008, Tekin and Tekdogan, 2015). Nowadays, the community-based planning is essential for the spatial regeneration processes. These realities generate a new sense of consciousness and a huge network of local activists, social enterprises able to develop the place in an incremental way. Therefore, it is import…