Search results for "DAS"

Understanding phenolic acids inhibition of α-amylase and α-glucosidase and influence of reaction conditions

2022

Phenolic acids are involved in modulating the activity of starch digestive enzymes but remains unclear if their interaction with enzymes or starch is governing the inhibition. The potential inhibition of nine phenolic acids against α-amylase and α-glucosidase was studied applying different methodologies to understand interactions between phenolic acids and either enzymes or substrates. Vanillic and syringic acids were prone to interact with α-amylase requiring low half-maximum inhibitory concentration (IC50) to inhibit starch hydrolysis. Nevertheless, the initial interaction of phenolic acids with starch somewhat obstructed their interaction with starch, requiring 10 times higher IC50, with…

Baltu filoloģija, 25 (2)

2017

Reelin and CXCL12 regulate distinct migratory behaviors during the development of the dopaminergic system.

2014

The proper functioning of the dopaminergic system requires the coordinated formation of projections extending from dopaminergic neurons in the substantia nigra (SN), ventral tegmental area (VTA) and retrorubral field to a wide array of forebrain targets including the striatum, nucleus accumbens and prefrontal cortex. The mechanisms controlling the assembly of these distinct dopaminergic cell clusters are not well understood. Here, we have investigated in detail the migratory behavior of dopaminergic neurons giving rise to either the SN or the medial VTA using genetic inducible fate mapping, ultramicroscopy, time-lapse imaging, slice culture and analysis of mouse mutants. We demonstrate that…

Xanthine dehydrogenase processes retinol to retinoic acid in human mammary epithelial cells

2008

Retinoic acid is considered to be the active metabolite of retinol, able to control differentiation and proliferation of epithelia. Retinoic acid biosynthesis has been widely described with the implication of multiple enzymatic activities. However, our understanding of the cell biological function and regulation of this process is limited. In a recent study we evidenced that milk xanthine oxidase (E.C. 1.17.3.2.) is capable to oxidize all-trans-retinol bound to CRBP (holo-CRBP) to all-trans-retinaldehyde and then to all-trans-retinoic acid. To get further knowledge regarding this process we have evaluated the biosynthetic pathway of retinoic acid in a human mammary epithelial cell line (HME…

Una llamativa pieza reacuñada de la ceca Carissa (Cortijo de Carija, Cádiz)

2014

Se documenta un curioso ejemplar pesado cuya noved ad, además de la pieza en sí, la representan los dos glóbulos observables detrás de la cabeza galeada y que encontramos en el anverso. Estos glóbulos, concebidos como marca de v alor, pudieron ser del cuño original del anverso o, en su caso, de una pieza anterior a una reacuñación; aunque, finalmente, cabría otra posibilidad y que descartamos en este artículo: un nuevo valor a añadir, como un “As” o una “Unidad”, para la entonces ceca gaditana de Carissa . We present a curious heavy specimen whose novelty resides, besides the piece itself, in the two noticeable pellets behind the galley pro w on obverse. These pellets, conceived as a mark o…

Development of type-specific and cross-reactive serological probes for the minor capsid protein of human papillomavirus type 33.

1993

Human papillomavirus type 33 (HPV33) is associated with malignant tumors of the cervix. In an attempt to develop immunological probes for HPV33 infections, antisera against various bacterial fusion proteins carrying sequences of the minor capsid protein encoded by L2 were raised in animals. Antigenic determinants on the HPV33 L2 protein were identified by using truncated fusion proteins and were classified as type specific or cross-reactive with respect to HPV1, -8, -11, -16, and -18. Cross-reactive epitopes map to amino acids 98 to 107 or to amino acids 102 to 112 and 107 to 117, respectively, depending on the fusion protein used for immunization. Antibodies directed toward these epitopes …

Pre- and Post-translational Regulation of Lysyl Oxidase by Transforming Growth Factor-β1 in Osteoblastic MC3T3-E1 Cells

1995

The final enzymatic step required for collagen cross-linking is the extracellular oxidative deamination of peptidyl-lysine and -hydroxylysine residues by lysyl oxidase. A cross-linked collagenous extracellular matrix is required for bone formation. The goals of this study were to compare the transforming growth factor (TGF)-beta 1 regulation of lysyl oxidase enzyme activity and steady state mRNA levels to changes in COL1A1 mRNA levels in MC3T3-E1 osteoblastic cells. TGF-beta 1 increased steady state lysyl oxidase and COL1A1 mRNA levels in a dose- and time-dependent manner. The increase in lysyl oxidase mRNA levels was transient, peaking at 12 h and 8.8 times controls in cells treated with 4…

Cre-lox: Target Sensitivity Matters

2019

Intraokulārā spiediena izmaiņas pēc refraktīvās lāzerkorekcijas

2015

Tēmas aktualitāte: Mūsdienas redzes korekcijas problēma ir ļoti aktuāla. Refraktīva ķirurģija paliek populāra jauniešu vidū, kas ir ļoti nozīmīgi. Iespējams, ka nākotnē pieaugs jaunu pacientu skaits un tāpēc svarīgi pievērst uzmanību pēc operatīvām izmaiņām, kas skar intraokulāru spiedienu. Mērķis: Noteikt intraokulāra spiediena izmaiņas pēc refraktīvas lāzerkorekcijas. Materiāli un metodes: Prospektīva pētījumā bija iesaistīti 50 pacienti, kuri ir veseli, dažāda vecumā un dzimuma, kuri vēlas veikt acu refraktīvu lāzerkorekciju. Pirms veikt ķirurģisku redzes korekciju pacientiem tika veikta pilna redzes orgānu izmeklēšana, tajā skaitā arī intraokulārā spiediena mērīšana un radzenes biezuma …

Fine analysis of the chromatin structure of the yeast SUC2 gene and of its changes upon derepression. Comparison between the chromosomal and plasmid-…

1987

Micrococcal nuclease digestion has been used to investigate some fine details of the chromatin structure of the yeast SUC2 gene for invertase. Precisely positioned nucleosomes have been found on a 2 kb sequence from the 3' non-coding region, and four nucleosomes also seem to occupy fixed positions on the 5' flank. Eleven nucleosomes lie on the coding region, although their positioning is not as precise as in the flanks. When the gene is derepressed, these latter nucleosomes adopt a more open conformation and so do two of the nucleosomes positioned on the 5' flank. A dramatic change occurs in the 3' flank, whose involvement in the structural transitions of chromatin upon gene activation is p…