Search results for "DICS"
showing 10 items of 2714 documents
DNA recovery from Droplet Digital™ PCR emulsions using liquid nitrogen
2020
Droplet microfluidics is a technology that enables the production and manipulation of small volumes. In biosciences, the most popular application of this technology is Droplet Digital™ PCR (ddPCR™), where parallel nanoliter-scale PCR assays are used to provide a high sensitivity and specificity for DNA detection. However, the recovery of PCR products for downstream applications such as sequencing can be challenging due to the droplets' stability. Here we compared five methods for disrupting the droplets to recover DNA. We found that rapid freezing in liquid nitrogen results in a clear phase separation and recovery of up to 70% of the DNA content. Liquid nitrogen freezing can thus offer a si…
Association of Arthroscopically-Assisted Latissimus Dorsi Tendon Transfer with Implantation of a Subacromial Balloon Spacer for Patients with Irrepar…
2022
Purpose. Massive irreparable posterosuperior rotator cuff tears in an active population, resulting in a pseudo-paralytic shoulder, pose a challenge for the orthopaedic surgeon. In an effort to avoid or delay arthroplasty surgery, other surgical strategies such as arthroscopically-assisted latissimus dorsi transfer (aLDT) or the implantation of a subacromial spacer (SAS) can be considered. The aim of the present study is to associate, for the first time, these two surgical procedures in order to demonstrate the surgical feasibility and the effectiveness of their synergistic biomechanical effect. Methods. The study group consisted of patients who underwent aLDT for a massive irreparable poste…
The “Journal of Functional Morphology and Kinesiology” Journal Club Series: Highlights on Recent Papers in Overtraining and Exercise Addiction
2019
We are glad to introduce the seventeenth Journal Club. This edition is focused on several relevant studies published in the last years in the field of Overtraining and Exercise Addiction, chosen by our Editorial Board members and their colleagues. We hope to stimulate your curiosity in this field and to share with you the passion for the sport seen also from the scientific point of view. The Editorial Board members wish you an inspiring lecture.
The “Journal of Functional Morphology and Kinesiology” Journal Club Series: Highlights on Recent Papers in Corrective Exercise
2020
We are glad to introduce the Journal Club of Volume Five, fourth Issue. This edition is focused on relevant studies published in the last few years in the field of corrective exercise, chosen by our Editorial Board members and their colleagues. We hope to stimulate your curiosity in this field and to share a passion for sport with you, seen also from the scientific point of view. The Editorial Board members wish you an inspiring lecture.
Pain neuroscience education and physical exercise for patients with chronic spinal pain in primary healthcare: a randomised trial protocol.
2019
AbstractBackgroundChronic musculoskeletal pain affects more than 20% of the population, and the prevalence is increasing, causing suffering, loss of quality of life, disability, and an enormous expenditure on healthcare resources. The most common location for chronic pain is the spine. Many of the treatments used are mainly passive (pharmacological and invasive) and poor outcomes. The treatments currently applied in the public health system do not comply with the recommendations of the main clinical practice guidelines, which suggest the use of educational measures and physical exercise as the first-line treatment. A protocol based on active coping strategies is described, which will be eva…
Flow Behavior of Chain and Star Polymers and Their Mixtures
2018
Star-shaped polymers show a continuous change of properties from flexible linear chains to soft colloids, as the number of arms is increased. To investigate the effect of macromolecular architecture on the flow properties, we employ computer simulations of single chain and star polymers as well as of their mixtures under Poiseuille flow. Hydrodynamic interactions are incorporated through the multi-particle collision dynamics (MPCD) technique, while a bead-spring model is used to describe the polymers. For the ultradilute systems at rest, the polymers are distributed uniformly in the slit channel, with a weak dependence on their number of arms. Once flow is applied, however, we find that the…
Effects of exercise and dietary interventions on serum metabolites in men with insomnia symptoms: A 6-month randomized controlled trial
2020
Abstract Accumulating evidence show that exercise and diet interventions are associated with improved sleep quality. Studies investigating the effects of exercise and dieting on circulating metabolomics in people with sleep disorders, particularly insomnia, are scarce. This 6-month randomized study aimed to assess the effects of exercise and dietary interventions on serum metabolites in men with insomnia symptoms. Seventy-two Finnish men (age: 51.6 ± 10.1 years) with chronic insomnia symptoms who were assigned to different intervention groups completed this study (exercise, n = 24; diet, n = 27; and control, n = 21). The Shapiro-Wilk W-test, Levene test, Spearman correlation analysis, and a…
Peak oxygen uptake cut-points to identify children at increased cardiometabolic risk - The PANIC Study.
2019
We aimed to develop cut -points for directly measured peak oxygen uptake (VO2peak ) to identify boys and girls at increased cardiometabolic risk using different scaling methods to control for body size and composition. Altogether 352 children (186 boys, 166 girls) aged 9 – 11 years were included in the analyses. We measured VO2peak directly during a maximal cycle ergometer exercise test and lean body mass (LM) by bioelectrical impedance. We computed a sex - and age -specific cardiometabolic risk score (CRS) by summing important cardiometabolic risk factors and defined increased cardiometabolic risk as >1 standard deviation above the mean of CRS. Receiver operating characteristics curves wer…
Me, osteogenesis imperfecta, and my classmates in physical education lessons: a case study of embodied pedagogy in action
2017
Pupils with disabilities have been found to experience a narrower physical education curriculum and participate less frequently than pupils without disabilities. A lack of knowledge, skills, relevant experiences and confidence amongst physical education (PE) teachers has been said to contribute to these differential educational experiences. This article adds to the paucity of research that analyses the PE experiences of pupils with disabilities while, at the same time, evaluating embodied pedagogy as a tool for better preparing PE teachers for their role as inclusive educators. Specifically, the article aims to: (1) explore the PE experiences of a university student named Violeta who lives …
Sport participation and vigilance in children: Influence of different sport expertise
2018
PURPOSE: The present study aimed to investigate the relationship between different types of sport expertise (externally-paced vs. self-paced sports) and vigilance performance in children by evaluating the cardiovascular fitness level of the participants. METHODS: Three groups of children (11.0 ± 0.2 years) differentiated in terms of their regular sport participation (football players, n = 20; track and field athletes, n = 20; non-athletic controls, n = 20) took part in the study. In one session, participants performed the Leger Multi-stage fitness test to estimate their aerobic fitness level. In another session, participants completed the Psychomotor Vigilance Task (PVT) to evaluate their v…