Search results for "DIR"
showing 10 items of 10242 documents
Secretion and antigenicity of hepatitis B virus small envelope proteins lacking cysteines in the major antigenic region.
1995
Abstract Disulfide bonds are of crucial importance for the structure and antigenic properties of the hepatitis B virus (HBV) envelope. We have evaluated the role of the eight highly conserved cysteines of the major antigenic region for assembly, secretion, and antigenicity of the envelope proteins. Mutants carrying single or multiple substitutions of alanine for cysteine were analyzed using epitope tagging and transient expression in COS-7 cells. The only single cysteines found to be indispensable for efficient secretion were Cys-107 and Cys-138, but double mutation of Cys-137 and Cys-139 also created a block to secretion. Poorly secreted mutants formed aberrant oligomeric structures. The a…
The glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase of Candida albicans is a surface antigen.
1997
A lambda gt11 cDNA library from Candida albicans ATCC 26555 was screened by using pooled sera from two patients with systemic candidiasis and five neutropenic patients with high levels of anti-C. albicans immunoglobulin M antibodies. Seven clones were isolated from 60,000 recombinant phages. The most reactive one contained a 0.9-kb cDNA encoding a polypeptide immunoreactive only with sera from patients with systemic candidiasis. The whole gene was isolated from a genomic library by using the cDNA as a probe. The nucleotide sequence of the coding region showed homology (78 to 79%) to the Saccharomyces cerevisiae TDH1 to TDH3 genes coding for glyceraldehyde-3-phosphate dehydrogenase (GAPDH), …
Secretion, interaction and assembly of two O-glycosylated cell wall antigens from Candida albicans.
2001
The mechanisms of incorporation of two antigens have been determined using a monoclonal antibody (3A10) raised against the material released from the mycelial cell wall by zymolyase digestion and retained on a concanavalin A column. One of the hybridomas secreted an IgG that reacted with two bands in Western blots. Indirect immunofluorescence showed that the antigens were located on the surfaces of mycelial cells, but within the cell walls of yeasts. These antigens were detected in a membrane preparation, in the SDS-soluble material and in the material released by a 1,3-beta-glucanase and chitinase from the cell walls of yeast and mycelial cells. In the latter three samples, an additional h…
Il deficit di tipicità delle interdittive antimafia
2020
In 2019, according to the report drawn up by the National Anti-Corruption Authority, they were banned 2044 Italian companies, of which 1458 based in the Southern Regions. An alarming fact which, if on the one hand, would seem acceptable to prevent the infiltration of associations mafia groups in the national economy in the public procurement system and in the loans disbursed by the State; on the other hand, it could reasonably be understood due to the very broad regulatory framework and articulated that characterizes the anti-mafia documentation system. The present work starting from the motivation system of the sentence of 5 September 2019, n. 6105 aims to investigate, first of all, the la…
The Fumarate/Succinate Antiporter DcuB of Escherichia coli Is a Bifunctional Protein with Sites for Regulation of DcuS-dependent Gene Expression
2008
DcuB of Escherichia coli catalyzes C4-dicarboxylate/succinate antiport during growth by fumarate respiration. The expression of genes of fumarate respiration, including the genes for DcuB (dcuB) and fumarate reductase (frdABCD) is transcriptionally activated by C4-dicarboxylates via the DcuS-DcuR two-component system, comprising the sensor kinase DcuS, which contains a periplasmic sensing domain for C4-dicarboxylates. Deletion or inactivation of dcuB caused constitutive expression of DcuS-regulated genes in the absence of C4-dicarboxylates. The effect was specific for DcuB and not observed after inactivation of the homologous DcuA or the more distantly related DcuC transporter. Random and s…
INTRODUZIONE AL D.LGS. 19 GENNAIO 2017, N. 3, DI ATTUAZIONE DELLA DIR. 2014/104/UE SUL RISARCIMENTO DEI DANNI PER VIOLAZIONE DELLA NORMATIVA ANTITRUST
2018
Con l’emanazione del d.lgs. 19 gennaio 2017, n. 3 e` stata data attuazione alla direttiva 2014/104/UE del Parlamento e del Consiglio del 26 novembre 2014, relativa a determinate norme che regolano le azioni per il risarcimento del danno ai sensi del diritto nazionale per violazioni delle disposizioni del diritto della concorrenza degli Stati membri e dell’Unione europea. Lo scritto, nell'introdurre una serie di commenti alla neo-introdotta normativa, esamina il percorso evolutivo che ha condotto all'emanazione della direttiva, le scelte con essa operate e le modalità di recepimento nell'ordinamento italiano. With the issuance of Legislative Decree January 19, 2017, no. 3 was implemented the…
L'Autorità garante della concorrenza e del mercato ei prgrammi di clemenza
2010
A distanza di tre anni dall'introduzione dei programmi di clemenza nella legge antitrust italiana, si impone una riflessione sui problemi aperti dalla loro adozione e applicazione. Il contributo li esamina, comparando il sistema italiano con quello comunitario e con gli altri sistemi vigenti nell'ambito dell'ECN. Evidenzia, quindi, alcuni aspetti critici della disciplina italiana, relativi all'accesso dei terzi alle dichiarazioni confessorie e alla documentazione allegata.
Autorità amministrative indipendenti e controllo di costituzionalità: considerazioni a partire dalla sentenza n. 13 del 2019 della Corte costituziona…
2020
The Italian Constitutional Court has been asked to decide on a constitutional incidental question referred to it by the Antitrust Authority. Rejecting the thesis of a quasi-judicial nature of the functions exercised by the Agcm and denying the existence of "gray areas" of constitutional justice, the the judgment no.13/2019 takes a position on the institutional placement of the Authorities within the administration. This, in addition to offering elements for reflections on the constitutional model of reference, requires important legal repercussions in terms of the effectiveness of the constitutional protection of connsidered interests.
The HCV Sicily Network: A web-based model for the management of HCV chronic liver diseases
2016
Epidemiological studies report that in Sicily reside about 30,000 citizens with a diagnosis of chronic hepatitis due to HCV. The availability of direct antiviral action (DAA) is a real therapeutic breakthrough, but the high cost of the therapeutic regimes limits their use and forced the National Health System to establish clinical priority for the treatment.The HCV Sicily Network is a web-based model of best medical practice, which was designed to improve the management and the treatment of HCV chronic hepatitis and cirrhosis. The network includes 41 centers and 84 gastroenterologists or infectivologists connected by a web platform that recorder the diagnosis and the clinic priority for the…
Cesare e l'elefante a teatro: un esempio di lezione su un 'caso culturale'.
2005
L'abitudine osservativa e l'attenzione simpatetica verso il mondo animale costituiscono la chiave di lettura del dialogo con 'l'altro' per eccellenza nel trafficato crocevia in cui si incontrano zoologia, antropologia, filosofia e diritto. A stabilire lo spazio di contiguità tra l'uomo e gli altri animali è la formula pliniana di matrice aristotelica, ad reliqua transeamus animalia (nat. hist. VIII), nella quale la figura dell'elefante, proximus humanis sensibus, si fa garante del transito fra etologia animale ed etologia umana.