Search results for "DLS"

showing 10 items of 22 documents

Amyloid Fibrils Formation in Concanavalin A studied by Dynamic Light Scattering and Fluorescence techniques

2007

DLS Thioflavin T Concanavalin A
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AMYLOID AGGREGATION IN CONCANAVALIN A AT HIGH PH STUDIED BY LIGHT SCATTERING, FLUORESCENCE AND CIRCULAR DICHROISM SPECTROSCOPY

2008

DLS Circular Dichroism thioflavin T
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Small, dense LDL: An update

2017

Purpose of review In this review, we summarize the latest findings on small, dense LDL (sdLDL) atherogenic particles, including their associations with other biomarkers. Recent findings Increased sdLDL levels have been reported not only in different metabolic disorders such as diabetes, obesity and metabolic syndrome, but also in patients with rheumatoid and psoriatic arthritis as well as hypothyroidism. A wide range of lipid-lowering, as well as other drug classes, including novel antidiabetic agents and nutraceuticals, exert favourable effects on these atherogenic particles. The 'gold standard' methodology for the assessment of sdLDL has not been established yet. However, the association …

cardiovascular riskmedicine.medical_specialtySmall dense ldl10265 Clinic for Endocrinology and Diabetology030209 endocrinology & metabolism610 Medicine & health030204 cardiovascular system & hematology2705 Cardiology and Cardiovascular Medicine03 medical and health scienceschemistry.chemical_compound0302 clinical medicineRisk FactorsInternal medicineDiabetes mellitusCardiovascular DiseaseMedicineHumansParticle SizeMetabolic Syndromebusiness.industryCholesterollipid-lowering therapieBiomarkerCholesterol LDLmedicine.diseaseAtherosclerosisObesityEndocrinologychemistrysmall dense LDLs: AtherosclerosiCardiovascular DiseasesCardiology and Cardiovascular MedicinebusinessBiomarkers
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Aqueous block copolymer-surfactant mixtures and their ability in solubilizing chlorinated organic compounds. A thermodynamic and SANS study.

2006

Within the topic of surfactant enhanced solubilization of additives sparingly soluble in water, volumetric, solubility, conductivity, and small-angle neutron scattering (SANS) experiments on mixtures composed of alpha,omega-dichloroalkane, surfactant, copolymer, and water were carried out at 298 K. The triblock copolymers (ethylene oxide)132(propylene oxide)50(ethylene oxide)132 (F108) and (ethylene oxide)76(propylene oxide)29(ethylene oxide)76 (F68) were chosen to investigate the role of the molecular weight keeping constant the hydrophilic/hydrophobic ratio. The selected surfactants are sodium decanoate (NaDec) and decyltrimethylammonium bromide (DeTAB) with comparable hydrophobicity and …

Aqueous solutionEthylene oxidescattering DLSConductivitySurfaces Coatings and Films| Micelleschemistry.chemical_compoundMonomerchemistryPulmonary surfactantChemical engineeringPolymer chemistryMaterials ChemistryCopolymerPropylene oxidePolyethylene oxidePhysical and Theoretical ChemistrySolubilityThe journal of physical chemistry. B
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Quantitative analysis of the impact of a human pathogenic mutation on the CCT5 chaperonin subunit using a proxy archaeal ortholog

2017

The human chaperonin complex is a ~ 1 MDa nanomachine composed of two octameric rings formed from eight similar but non-identical subunits called CCT. Here, we are elucidating the mechanism of a heritable CCT5 subunit mutation that causes profound neuropathy in humans. In previous work, we introduced an equivalent mutation in an archaeal chaperonin that assembles into two octameric rings like in humans but in which all subunits are identical. We reported that the hexadecamer formed by the mutant subunit is unstable with impaired chaperoning functions. This study quantifies the loss of structural stability in the hexadecamer due to the pathogenic mutation, using differential scanning calorim…

0301 basic medicineProtein subunitMutantBiophysicsHeterologousBiochemistryChaperoninChaperoninlcsh:Biochemistry03 medical and health sciencesDSC differential scanning calorimetryCCT% chaperoninPf Pyrococcus furiosusDenaturation (biochemistry)lcsh:QD415-436Molecular Biologylcsh:QH301-705.5DLS dynamic light scatteringbiologyITC isothermal titration calorimetryWild typeIsothermal titration calorimetryCell BiologyChaperonopathiesbiology.organism_classificationProtein calorimetryNeuropathyPyrococcus furiosus030104 developmental biologyBiochemistryBiophysiclcsh:Biology (General)Pyrococcus furiosusChaperonopathieCCT5; Chaperonin; Chaperonopathies; Neuropathy; Protein calorimetry; Pyrococcus furiosus; Biophysics; Biochemistry; Molecular Biology; Cell BiologyCCT5Pyrococcus furiosuResearch ArticlePf-CD1 Pyrococcus furiosus chaperonin subunit with the last 22 amino acids deletedBiochemistry and Biophysics Reports
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An Advanced Sensor for Particles in Gases Using Dynamic Light Scattering in Air as Solvent

2021

Dynamic Light Scattering is a technique currently used to assess the particle size and size distribution by processing the scattered light intensity. Typically, the particles to be investigated are suspended in a liquid solvent. An analysis of the particular conditions required to perform a light scattering experiment on particles in air is presented in detail, together with a simple experimental setup and the data processing procedure. The results reveal that such an experiment is possible and using the setup and the procedure, both simplified to extreme, enables the design of an advanced sensor for particles and fumes that can output the average size of the particles in air.

Materials scienceTP1-118501 natural sciencesBiochemistryArticleLight scatteringAnalytical Chemistry010309 opticsparticle sizingOpticsDynamic light scattering0103 physical sciencesElectrical and Electronic Engineeringair DLSInstrumentationSIMPLE (dark matter experiment)Data processingbusiness.industryChemical technology010401 analytical chemistrydynamic light scatteringAtomic and Molecular Physics and Optics0104 chemical sciencesSolventadvanced fire sensorParticle sizeScattered lightbusinessIntensity (heat transfer)Sensors
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Low-density lipoproteins generated during an oral fat load in mild hypertriglyceridemic and healthy subjects are smaller, denser, and have an increas…

2005

Triglyceride-rich lipoproteins generated during the postprandial phase are atherogenic. Large very low-density lipoproteins (LDLs) or chylomicrons (CMs) are not as atherogenic as their remnants (Rem). Small and dense LDLs are associated with cardiovascular disease. Low-density lipoprotein size is partly under genetic control and is considered as a relatively stable LDL feature. In this article, we present data on retinyl palmitate kinetics correlated with the modification of LDL features in terms of size, density, and in vitro receptor binding affinity after an oral fat load. Six nondiabetic, hypertriglyceridemic (HTG) patients and 6 healthy controls were examined. Low-density lipoprotein s…

AdultMalemedicine.medical_specialtyRetinyl EstersSettore MED/09 - Medicina InternaEndocrinology Diabetes and MetabolismBinding CompetitiveModels Biologicalchemistry.chemical_compoundEndocrinologyInternal medicineRetinyl palmitateCell Line TumorChylomicronsmedicineHumansReceptorVitamin AHypertriglyceridemiaLow-density lipoproteins hypertriglyceridemia Fasting and postprandial LDLsTriglycerideCatabolismChemistryFastingFibroblastsPostprandial PeriodDietary FatsLipidsLipoproteins LDLKineticsEndocrinologyPostprandialReceptors LDLlipids (amino acids peptides and proteins)Density gradient ultracentrifugationElectrophoresis Polyacrylamide GelFemaleDiterpenesUltracentrifugationLipoproteinChylomicronMetabolism: clinical and experimental
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Profiling Suspensions in Natural Water by a Simplified Dynamic Light Scattering Procedure and Sedimentation

2015

Abstract A coherent light scattering experiment was carried out. The samples were aqueous natural water suspensions picked from the same river. While sedimentation occurred in the samples, they were subjected to a dynamic light scattering (DLS) experiment and the time series was recorded at certain time intervals. For each recording, a program written for this purpose, performing at least square minimisation, computed the average diameter of the particles in suspension. The variation of the average diameter in time indicates the dominant type of suspensions in water.

0106 biological sciencesMaterials scienceAqueous solutionEcologyAverage diameterScattering010604 marine biology & hydrobiologyNatural waterMineralogy010501 environmental sciences01 natural sciencescoherent light scatteringDynamic light scatteringsuspensionsnatural waterdynamic light scattering (dls)QH540-549.50105 earth and related environmental sciencesTransylvanian Review of Systematical and Ecological Research
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Mass Action Model Applied to the Thermodynamic Properties of Transfer of Nonionic Copolymers from Water to the Aqueous Surfactant Solutions

2003

A thermodynamic model which enables the properties of aqueous copolymer/surfactant mixtures to be fit quantitatively was proposed. Namely, a relationship between the properties of transfer of the unassociated copolymer from water to the aqueous surfactant solutions (DeltaY(t)) and the surfactant concentration was derived. The model was based on the idea that AY, can be expressed in terms of the following contributions: (1) interaction between monomers of copolymer and surfactant, (2) displacement of the monomer-micelle equilibrium induced by the copolymer, (3) formation of the surfactant-copolymer aggregation complex, and (4) formation of the mixed micelles. Such a model was applied to most…

Aqueous solutionChemistryInorganic chemistrySurfaces Coatings and FilmsCondensed Matter::Soft Condensed MatterThermodynamic modelPulmonary surfactantChemical engineeringMaterials ChemistryCopolymerPhysics::Chemical PhysicsPhysical and Theoretical ChemistryAction modelPolyethylene oxides Micelles scattering DLSThe Journal of Physical Chemistry B
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Using Dynamic Light Scattering for Monitoring the Size of the Suspended Particles in Wastewater

2019

Abstract A coherent light scattering experiment on wastewater samples extracted from several stages of water processing within a wastewater processing plant was carried out. The samples were allowed to sediment while they were the subject of a Dynamic Light Scattering (DLS) measurement. The recorded time series were processed using an Artificial Neural Network based DLS procedure to produce the average diameter of the particles in suspension. The method, using a single physical procedure for monitoring the variation of the average diameter in time, indicates the dominant type of suspensions in water.

Materials scienceAverage diameterEcologyScatteringSuspended particlesWater processingSediment010501 environmental sciences01 natural sciencescoherent light scatteringSuspension (chemistry)010309 opticsWastewaterDynamic light scattering0103 physical sciencesBiological systemdynamic light scattering (dls)wastewaterartificial neural networkQH540-549.50105 earth and related environmental sciencesTransylvanian Review of Systematical and Ecological Research
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