Search results for "DOMAIN"
showing 10 items of 2485 documents
Domain shuffling between Vip3Aa and Vip3Ca: chimera stability and insecticidal activity against European, American, African, and Asian pests
2020
The bacterium Bacillus thuringiensis produces insecticidal Vip3 proteins during the vegetative growth phase with activity against several lepidopteran pests. To date, three different Vip3 protein families have been identified based on sequence identity: Vip3A, Vip3B, and Vip3C. In this study, we report the construction of chimeras by exchanging domains between Vip3Aa and Vip3Ca, two proteins with marked specificity differences against lepidopteran pests. We found that some domain combinations made proteins insoluble or prone to degradation by trypsin as most abundant insect gut protease. The soluble and trypsin-stable chimeras, along with the parental proteins Vip3Aa and Vip3Ca, were tested…
The Poisson problem: A comparison between two approaches based on SPH method
2012
Abstract In this paper two approaches to solve the Poisson problem are presented and compared. The computational schemes are based on Smoothed Particle Hydrodynamics method which is able to perform an integral representation by means of a smoothing kernel function by involving domain particles in the discrete formulation. The first approach is derived by means of the variational formulation of the Poisson problem, while the second one is a direct differential method. Numerical examples on different domain geometries are implemented to verify and compare the proposed approaches; the computational efficiency of the developed methods is also studied.
MMGBSA As a Tool To Understand the Binding Affinities of Filamin–Peptide Interactions
2013
Filamins (FLN) are large dimeric proteins that cross-link actin and work as important scaffolds in human cells. FLNs consist of an N-terminal actin-binding domain followed by 24 immunoglobulin-like domains (FLN1-24). FLN domains are divided into four subgroups based on their amino acid sequences. One of these subgroups, including domains 4, 9, 12, 17, 19, 21, and 23, shares a similar ligand-binding site between the β strands C and D. Several proteins, such as integrins β2 and β7, glycoprotein Ibα (GPIbα), and migfilin, have been shown to bind to this site. Here, we computationally estimated the binding free energies of filamin A (FLNa) subunits with bound peptides using the molecular mechan…
A KST-BASED SYSTEM FOR STUDENT TUTORING
2008
Abstract: A novel assessment procedure based on knowledge space theory (KST) is presented along with a complete implementation of an intelligent tutoring system. (ITS) that has been used to test our theoretical findings. The key idea is that correct assessment of the student knowledge is strictly related to the structure of the domain ontology. Suitable relationships between the concepts must be present to allow the creation of a reverse path from the "knowledge state" representing the student goal to the one that contains her actual knowledge about this topic. Knowledge space theory is a very good framework to guide the process of building the ontology used, by the artificial tutor The sys…
Defining Interaction Design Patterns to Extract Knowledge from Big Data
2018
[EN] The Big Data domain offers valuable opportunities to gain valuable knowledge. The User Interface (UI), the place where the user interacts to extract knowledge from data, must be adapted to address the domain complexities. Designing UIs for Big Data becomes a challenge that involves identifying and designing the user-data interaction implicated in the knowledge extraction. To design such an interaction, one widely used approach is design patterns. Design Patterns describe solutions to common interaction design problems. This paper proposes a set of patterns to design UIs aimed at extracting knowledge from the Big Data systems data conceptual schemas. As a practical example, we apply the…
Mental contents in interacting with a multiobjective optimization program
2008
User psychology aims at understanding human-machine interaction from a psychological point of view. Its ultimate goal is to provide knowledge about human psychological properties for interaction designers. In this article, we are particularly interested in applying the theoretical concepts of mental contents (i.e., the information contents of users’ mental representations), in studying interaction with professional software. The immediate motivation for adopting such an approach arises from problems met in designing interaction processes in multiobjective optimization software. These types of software are meant to support complex thought and decision-making processes and this is why interac…
Motivations and domain specificity in intercultural adaptation : bloggers' experiences in their new host countries
2014
The current qualitative study investigates immigrant’s intrinsic (internal) and extrinsic (external) motivations and how they surface in the intercultural adaption process. Additionally, it aims to answer whether immigrant’s adaptation orientations differ between private and public (life) domains by testing Boski’s (2008) IAFS model [the model of integration as functional(partial) specialization]. It is a fairly recent model of adaptation and only few prior studies have utilized it. The study concentrates on voluntary migrants, individuals, who have made a personal choice to leave their homelands. In the current study naturally occurring data in a form of weblogs were utilized as research d…
Modellierung überbetrieblicher behandlungsprozesse durch objekt-petrinetze
2005
Clinical processes are often performed by the cooperation of different healthcare organization. But even that for modeling of such processes the object Petri nets can be used, they often lack of intuitive comprehensibility. This contribution presents an interpretation of object Petri nets which assigns abstract net elements to real objects of the healthcare domain. By doing this it helps domain user to apply this Petri net type to the modeling of clinical processes. The application of this approach is demonstrated.
Typology of pentad circulation anomalies over the Eastern Africa - Western Indian Ocean region, and their relationship with rainfall
2005
International audience; The aim of this study was to classify the most frequently observed atmospheric circula- tion anomaly patterns in eastern Africa and the adjacent Indian Ocean. As an example of the useful- ness of such a classification, the second objective was to test whether these patterns account for intraseasonal rainfall anomalies in the region. A partitioning algorithm, known as dynamical cluster analysis, was therefore applied to the zonal (U)and meridional (V)components of the wind anom- alies, obtained from the NCEP-NCAR Reanalysis R-2 at the pentad (5 d) timescale. The 3 geopoten- tial levels 850, 700 and 200 hPa were combined. Focus is on the transition seasons (March to Ma…
Tracking Ca
2019
We characterize thus-far elusive domain rearrangements of a calcium-transporting ATPase in the native membrane.