Search results for "DOMAIN"
showing 10 items of 2485 documents
Classes of non-conventional tetraspanins defined by alternative splicing
2019
AbstractTetraspanins emerge as a family of membrane proteins mediating an exceptional broad diversity of functions. The naming refers to their four transmembrane segments, which define the tetraspanins‘ typical membrane topology. In this study, we analyzed alternative splicing of tetraspanins. Besides isoforms with four transmembrane segments, most mRNA sequences are coding for isoforms with one, two or three transmembrane segments, representing structurally mono-, di- and trispanins. Moreover, alternative splicing may alter transmembrane topology, delete parts of the large extracellular loop, or generate alternative N- or C-termini. As a result, we define structure-based classes of non-con…
LC–MS Based Cleavage Site Profiling of the Proteases ADAM10 and ADAM17 Using Proteome-Derived Peptide Libraries
2014
A Disintegrin and Metalloproteinase 10 (ADAM10) and ADAM17 catalyze ectodomain shedding of a number of cell surface proteins important for embryonic development and tissue homeostasis. Changes in the expression levels or dysregulated proteolytic activity of ADAM10 and ADAM17 have been shown to play important roles in multiple diseases such as inflammation, cancer, and neurodegenerative disorders. Despite the well documented substrate repertoire of ADAM10 and ADAM17, little is known about their cleavage site specificity. We optimized Q-PICS (Quantitative Proteomics for the Identification of Cleavage Sites) to elucidate the cleavage site specificity of recombinant murine ADAM10 and ADAM17. Tw…
Membrane vesicles containing matrix metalloproteinase-9 and fibroblast growth factor-2 are released into the extracellular space from mouse mesoangio…
2010
Certain proteins, including fibroblast growth factor-2 (FGF-2) and matrix metalloproteinase-9 (MMP-9), have proved very effective in increasing the efficacy of mesoangioblast stem cell therapy in repairing damaged tissue. We provide the first evidence that mouse mesoangioblast stem cells release FGF-2 and MMP-9 in their active form through the production of membrane vesicles. These vesicles are produced and turned over continuously, but are stable for some time in the extracellular milieu. Mesoangioblasts shed membrane vesicles even under oxygen tensions that are lower than those typically used for cell culture and more like those of mouse tissues. These findings suggest that mesoangioblast…
An efficient distributed approach for dynamic multicast trees
2005
In this paper we present a distributed algorithm for constructing efficient multicast trees in large networks. The algorithm allows the set up of effective distribution trees using a coordination protocol among the network nodes. The algorithm has been implemented and extensively tested both in simulation, and on experimental networks. Performance evaluation indicates that our approach outperforms the state-of-the-art distributed algorithms for multicast tree setup, providing good levels of convergence time and communication complexity. Furthermore, we introduce a distributed technique for dynamically updating the multicast tree. The approach monitors the accumulated damage as nodes join or…
Pressure-tuning of magnetism and linkage isomerism in iron(II) hexacyanochromate.
2005
A pressure-induced linkage isomerization of the cyanide anion has been observed in single crystals of a chromium(III)-iron(II) Prussian blue analogue of formula K0.4Fe4[Cr(CN)6]2.8 square1.2.16H2O (1). Upon application of pressure in the 0-1200 MPa range, the cyanide ligand rotates and becomes C-bonded to the iron(II) cations, leading to a stabilization of their diamagnetic low-spin states. The result is a decrease of magnetization and magnetic ordering temperatures from TC = 19 K at ambient pressure to 13 K at 1200 MPa. The initial magnetic properties can be restored on pressure release. The reversible movement of cyanide in the solid state can be exploited as a switch of the magnetic inte…
Domain Generation Algorithm Detection Using Machine Learning Methods
2018
A botnet is a network of private computers infected with malicious software and controlled as a group without the knowledge of the owners. Botnets are used by cybercriminals for various malicious activities, such as stealing sensitive data, sending spam, launching Distributed Denial of Service (DDoS) attacks, etc. A Command and Control (C&C) server sends commands to the compromised hosts to execute those malicious activities. In order to avoid detection, recent botnets such as Conficker, Zeus, and Cryptolocker apply a technique called Domain-Fluxing or Domain Name Generation Algorithms (DGA), in which the infected bot periodically generates and tries to resolve a large number of pseudorando…
The relationship of symptom dimensions with premorbid adjustment and cognitive characteristics at first episode psychosis: Findings from the EU-GEI s…
2021
Premorbid functioning and cognitive measures may reflect gradients of developmental impairment across diagnostic categories in psychosis. In this study, we sought to examine the associations of current cognition and premorbid adjustment with symptom dimensions in a large first episode psychosis (FEP) sample. We used data from the international EU-GEI study. Bifactor modelling of the Operational Criteria in Studies of Psychotic Illness (OPCRIT) ratings provided general and specific symptom dimension scores. Premorbid Adjustment Scale estimated premorbid social (PSF) and academic adjustment (PAF), and WAIS-brief version measured IQ. A MANCOVA model examined the relationship between symptom di…
Rho protein inactivation induced apoptosis of cultured human endothelial cells.
2002
Small GTP-binding Rho GTPases regulate important signaling pathways in endothelial cells, but little is known about their role in endothelial cell apoptosis. Clostridial cytotoxins specifically inactivate GTPases by glucosylation [ Clostridium difficile toxin B-10463 (TcdB-10463), C. difficile toxin B-1470 (TcdB-1470)] or ADP ribosylation ( C. botulinum C3 toxin). Exposure of human umbilical cord vein endothelial cells (HUVEC) to TcdB-10463, which inhibits RhoA/Rac1/Cdc42, or to C3 toxin, which inhibits RhoA, -B, -C, resulted in apoptosis, whereas inactivation of Rac1/Cdc42 with TcdB-1470 was without effect, suggesting that Rho inhibition was responsible for endothelial apoptosis. Disruptio…
Representation Theorems for Solvable Sesquilinear Forms
2017
New results are added to the paper [4] about q-closed and solvable sesquilinear forms. The structure of the Banach space $\mathcal{D}[||\cdot||_\Omega]$ defined on the domain $\mathcal{D}$ of a q-closed sesquilinear form $\Omega$ is unique up to isomorphism, and the adjoint of a sesquilinear form has the same property of q-closure or of solvability. The operator associated to a solvable sesquilinear form is the greatest which represents the form and it is self-adjoint if, and only if, the form is symmetric. We give more criteria of solvability for q-closed sesquilinear forms. Some of these criteria are related to the numerical range, and we analyse in particular the forms which are solvable…
Existence and almost uniqueness for p -harmonic Green functions on bounded domains in metric spaces
2020
We study ($p$-harmonic) singular functions, defined by means of upper gradients, in bounded domains in metric measure spaces. It is shown that singular functions exist if and only if the complement of the domain has positive capacity, and that they satisfy very precise capacitary identities for superlevel sets. Suitably normalized singular functions are called Green functions. Uniqueness of Green functions is largely an open problem beyond unweighted $\mathbf{R}^n$, but we show that all Green functions (in a given domain and with the same singularity) are comparable. As a consequence, for $p$-harmonic functions with a given pole we obtain a similar comparison result near the pole. Various c…