Search results for "DOMAIN"
showing 10 items of 2485 documents
An ontological infrastructure for the automatic control of freight transport
2008
Currently, freight transport carried through a country or through different countries undergoes many delays due to merchandise controls. Merchandise control is only carried out at some border posts or when the authorities demand it. Such control can be an important problem since there are different laws for the same merchandise, according to the legislation of the country and even within the same country. Nowadays there are vocabularies or languages that describe concepts and structures of data related to transport, but the description is just syntactic, not semantic. Therefore the objective to be reached in this part of the research has been to develop a representation scheme of a particul…
Binding of PTEN to specific PDZ domains contributes to PTEN protein stability and phosphorylation by microtubule-associated serine/threonine kinases
2005
The tumor suppressor phosphatase PTEN is a key regulator of cell growth and apoptosis that interacts with PDZ domains from regulatory proteins, including MAGI-1/2/3, hDlg, and MAST205. Here we identified novel PTEN-binding PDZ domains within the MAST205-related proteins, syntrophin-associated serine/threonine kinase and MAST3, characterized the regions of PTEN involved in its interaction with distinctive PDZ domains, and analyzed the functional consequences on PTEN of PDZ domain binding. Using a panel of PTEN mutations, as well as PTEN chimeras containing distinct domains of the related protein TPTE, we found that the PTP and C2 domains of PTEN do not affect PDZ domain binding and that the …
Direct Activation of Bax by p53 Mediates Mitochondrial Membrane Permeabilization and Apoptosis
2004
The tumor suppressor p53 exerts its anti-neoplastic activity primarily through the induction of apoptosis. We found that cytosolic localization of endogenous wild-type or trans-activation–deficient p53 was necessary and sufficient for apoptosis. p53 directly activated the proapoptotic Bcl-2protein Bax in the absence of other proteins to permeabilize mitochondria and engage the apoptotic program. p53 also released both proapoptotic multidomain proteins and BH3-only proteins [Proapoptotic Bcl-2family proteins that share only the third Bcl-2homology domain (BH3)] that were sequestered by Bcl-xL. The transcription-independent activation of Bax by p53 occurred with similar kinetics and concentra…
Selective Binding of Collagen Subtypes by Integrin α1I, α2I, and α10I Domains
2001
Four integrins, namely alpha(1)beta(1), alpha(2)beta(1), alpha(10)beta(1), and alpha(11)beta(1), form a special subclass of cell adhesion receptors. They are all collagen receptors, and they recognize their ligands with an inserted domain (I domain) in their alpha subunit. We have produced the human integrin alpha(10)I domain as a recombinant protein to reveal its ligand binding specificity. In general, alpha(10)I did recognize collagen types I-VI and laminin-1 in a Mg(2+)-dependent manner, whereas its binding to tenascin was only slightly better than to albumin. When alpha(10)I was tested together with the alpha(1)I and alpha(2)I domains, all three I domains seemed to have their own collag…
Identifying the Optimum Social Site that Could Serve eLearning Purposes: A Preliminary Analysis
2020
Nowadays, an increasingly attractive research domain is represented by the integration of eLearning systems with the Social Media tools. However, identifying the proper Social Media platform to serve educational purposes could be an extremely hard task, mainly due to the variations from platform to platform. Thus, the first aim of this article is to identify the optimum typology that could be successfully integrated with eLearning systems. Moving forward, a classification of the top 20 most popular Social Media sites at global level in terms of their main objectives will be required in order to asses a specific typology of each of this. Thereafter, we propose a preliminary model of comparis…
NMR structure of a non-conjugatable, ADP-ribosylation associated, ubiquitin-like domain from Tetrahymena thermophila polyubiquitin locus.
2019
Abstract Background Ubiquitin-like domains (UbLs), in addition to being post-translationally conjugated to the target through the E1-E2-E3 enzymatic cascade, can be translated as a part of the protein they ought to regulate. As integral UbLs coexist with the rest of the protein, their structural properties can differ from canonical ubiquitin, depending on the protein context and how they interact with it. In this work, we investigate T.th-ubl5, a UbL present in a polyubiquitin locus of Tetrahymena thermophila, which is integral to an ADP-ribosyl transferase protein. Only one other co-occurrence of these two domains within the same protein has been reported. Methods NMR, multiple sequence al…
Domēnspecifisku rīku konfigurācijas valoda un tās realizācija
2014
Promocijas darbā ir izstrādāts konfigurators plaša profila DSML rīku definēšanai. Konfigurators balstās uz ideju, ka DSML definīciju var uzdot ar universālu metamodeli (UML klašu diagrammas formā), kura pēc tam konfiguratorā tiek apstrādāta, lai nodrošinātu rīka darbību. Turklāt nestandarta gadījumiem, kad esošās metamodeļa iespējas ir nepietiekošas, metamodelī ir realizēts paplašinājuma punktu mehānisms, kas ļauj pievienot rīkam specifiski transformāciju valodā noprogrammētu funkcionalitāti. Izstrādātais konfigurators ir pārbaudīts vairākos praktiskos lietojumos un apliecinājis savu efektivitāti.
Modeling and Query Language for Hospitals
2013
So far the traditional process modeling languages have found a limited use in the hospital settings. One of the reasons behind this delay has been the lack of clear definition of the sequence of activities that are carried out in the hospital. We propose a new modeling language (as a profile of UML Class diagrams) that captures all the useful features from various UML diagrams and can be used in modeling of the hospitals. Based on the modeling language, we have developed an easy-to-perceive graphical query language, which allows the physicians to retrieve directly from the various hospital databases information they need to better understand the flow of clinical processes.
Alix protein is substrate of Ozz-E3 ligase and modulates actin remodeling in skeletal muscle
2012
Alix/AIP1 is a multifunctional adaptor protein that participates in basic cellular processes, including membrane trafficking and actin cytoskeleton assembly, by binding selectively to a variety of partner proteins. However, the mechanisms regulating Alix turnover, subcellular distribution, and function in muscle cells are unknown. We now report that Alix is expressed in skeletal muscle throughout myogenic differentiation. In myotubes, a specific pool of Alix colocalizes with Ozz, the substrate-binding component of the muscle-specific ubiquitin ligase complex Ozz-E3. We found that interaction of the two endogenous proteins in the differentiated muscle fibers changes Alix conformation and pro…
dsRNA induces apoptosis through an atypical death complex associating TLR3 to caspase-8
2012
Toll-like receptor 3 (TLR3) is a pattern-recognition receptor known to initiate an innate immune response when stimulated by double-stranded RNA (dsRNA). Components of TLR3 signaling, including TIR domain-containing adapter inducing IFN-α (TRIF), have been demonstrated to contribute to dsRNA-induced cell death through caspase-8 and receptor interacting protein (RIP)1 in various human cancer cells. We provide here a detailed analysis of the caspase-8 activating machinery triggered in response to Poly(I:C) dsRNA. Engagement of TLR3 by dsRNA in both type I and type II lung cancer cells induces the formation of an atypical caspase-8-containing complex that is devoid of classical death receptors…