Search results for "DOMAIN"
showing 10 items of 2485 documents
Toll signal transduction pathway in bivalves: Complete cds of intermediate elements and related gene transcription levels in hemocytes of immune stim…
2014
Based on protein domain structure and organization deduced from mRNA contigs, 15 transcripts of the Toll signaling pathway have been identified in the bivalve, Mytilus galloprovincialis. Identical searches performed on publicly available Mytilus edulis ESTs revealed 11 transcripts, whereas searches performed in genomic and new transcriptome sequences of the Pacific oyster, Crassostrea gigas, identified 21 Toll-related transcripts. The remarkable molecular diversity of TRAF and IKK coding sequences of C. gigas, suggests that the sequence data inferred from Mytilus cDNAs may not be exhaustive. Most of the Toll pathway genes were constitutively and ubiquitously expressed in M. galloprovinciali…
Polarisation dependent Raman study of single-crystal nickel oxide
2011
Abstract The magnetic domain structure and Raman scattering have been studied in NiO single-crystals with three different (100), (110) and (111) orientations. Twin-domain structure was observed in NiO(100) and NiO(110) single-crystals using cross-polarized optical microscopy. We found that the ratio of the two-magnon (at 1500 cm−1) to the two-phonon (2LO, at 1100 cm−1) Raman bands intensity is sensitive in a particular way to the type of the twin-domain pattern.
Smad7 controls resistance of colitogenic T cells to regulatory T cell-mediated suppression.
2008
Background & Aims Foxp3-expressing regulatory T cells (Tregs) play a key role in the maintenance of the gut immune homeostasis, and an intact transforming growth factor (TGF)-β signaling is required for their function. In inflammatory bowel disease (IBD), the TGF-β signaling is impaired because of high expression of the inhibitory molecule Smad7. Although no intrinsic defects in Tregs function have been shown in IBD, it is still unknown whether colitogenic T cells are susceptible to Treg-mediated suppression. In this study, we have investigated whether IBD mucosal CD4+ T cells are resistant to Tregs and whether Smad7 is involved in this process. Methods IBD lamina propria mononuclear cells …
Apprentissage implicite de régularités : Mise en évidence d'une différence d'apprentissage entre tâches motrices continues et discrètes
2005
This research is concerned with the implicit learning of regularities in continuous motor tasks (e.g., tracking tasks) and discrete tasks (e.g., Serial Reaction Time tasks). First, a reanalysis of Wulf and collaborator's studies, dealing with implicit motor learning, made it possible to highlight methodological problems and potential biases that provide alternative explanations for the apparent implicit learning obtained in their continuous tracking experiments. Indeed, by respecting a more rigorous methodology, it appears that it is quite difficult to obtain such learning in a continuous task. This led us to try to understand why it was easier to draw benefit from a repetition in a discret…
The deubiquitinase USP11 is a versatile and conserved regulator of autophagy
2021
Autophagy is a major cellular quality control system responsible for the degradation of proteins and organelles in response to stress and damage to maintain homeostasis. Ubiquitination of autophagy-related proteins or regulatory components is important for the precise control of autophagy pathways. Here, we show that the deubiquitinase ubiquitin-specific protease 11 (USP11) restricts autophagy and that KO of USP11 in mammalian cells results in elevated autophagic flux. We also demonstrate that depletion of the USP11 homolog H34C03.2 in Caenorhabditis elegans triggers hyperactivation of autophagy and protects the animals against human amyloid-β peptide 42 aggregation-induced paralysis. USP11…
The increase in maternal expression of axin1 and axin2 contribute to the zebrafish mutant ichabod ventralized phenotype.
2014
β-Catenin is a central effector of the Wnt pathway and one of the players in Ca(+)-dependent cell-cell adhesion. While many wnts are present and expressed in vertebrates, only one β-catenin exists in the majority of the organisms. One intriguing exception is zebrafish that carries two genes for β-catenin. The maternal recessive mutation ichabod presents very low levels of β-catenin2 that in turn affects dorsal axis formation, suggesting that β-catenin1 is incapable to compensate for β-catenin2 loss and raising the question of whether these two β-catenins may have differential roles during early axis specification. Here we identify a specific antibody that can discriminate selectively for β-…
Occlusion-derived baculovirus : interaction with human hepatocarcinoma cells and application of baculovirus display technology
2007
Experimental Crystallization of a High-K Arc Basalt: the Golden Pumice, Stromboli Volcano (Italy)
2006
International audience; The near-liquidus crystallization of a high-K basalt (PST-9 golden pumice, 49·4 wt % SiO2, 1·85 wt % K2O, 7·96 wt % MgO) from the present-day activity of Stromboli (Aeolian Islands, Italy) has been experimentally investigated between 1050 and 1175°C, at pressures from 50 to 400 MPa, for melt H2O concentrations between 1·2 and 5·5 wt % and {Delta}NNO ranging from –0·07 to +2·32. A drop-quench device was systematically used. AuPd alloys were used as containers in most cases, resulting in an average Fe loss of 13% for the 34 charges studied. Major crystallizing phases include clinopyroxene, olivine and plagioclase. Fe–Ti oxide was encountered in a few charges. Clinopyro…
Thermodynamic analysis of the interactions between human ACE2 and spike RBD of Betacoronaviruses (SARS-CoV-1 and SARS-CoV-2)
2023
There are many scientific reports on the interaction of the SARS-CoV-2 virus S protein (and its RBD) with the human ACE2 receptor protein. However, there are no reliable data on how this interaction differs from the interaction of the receptor binding domain of SARS-CoV-1 with ACE2, in terms of binding strength and changes in reaction enthalpy and entropy. Our studies have revealed these differences and the impact of zinc ions on this interaction. Intriguingly, the binding affinity of both RBDs (of SARS-CoV-1 and of SARS-CoV-2) to the ACE2 receptor protein is almost identical; however, there are some differences in the entropic and enthalpic contributions to these interactions.
High Resolution Solution NMR Structure of the Z Domain of Staphylococcal Protein A. Analysis of Secondary Structure for Free Z Domain and Bounded to …
1997
Staphylococcal protein A (SpA) is a cell-wall-bound pathogenicity factor from the bacterium Staphylcoccus aureus. It exhibits tight binding to many IgG, IgA and IgM molecules at site(s) different from antigen-combining site. Because of their small size and immunoglobulin (IgG)-binding activities, domains of protein A are important targets for protein engineering efforts and for the development of computational approaches for de novo protein folding.