Search results for "DRY"
showing 10 items of 980 documents
Rethinking Cysteine Protective Groups:S-Alkylsulfonyl-l-Cysteines for Chemoselective Disulfide Formation
2016
The ability to reversibly cross-link proteins and peptides grants the amino acid cysteine its unique role in nature as well as in peptide chemistry. We report a novel class of S-alkylsulfonyl-l-cysteines and N-carboxy anhydrides (NCA) thereof for peptide synthesis. The S-alkylsulfonyl group is stable against amines and thus enables its use under Fmoc chemistry conditions and the controlled polymerization of the corresponding NCAs yielding well-defined homo- as well as block co-polymers. Yet, thiols react immediately with the S-alkylsulfonyl group forming asymmetric disulfides. Therefore, we introduce the first reactive cysteine derivative for efficient and chemoselective disulfide formation…
Genotoxic and endocrine activities of bis(hydroxyphenyl)methane (bisphenol F) and its derivatives in the HepG2 cell line
2008
International audience; Human can be exposed to bis(hydroxyphenyl)methane (bisphenol F or BPF) and its derivatives as environment and food's contaminants. This study was investigated to identify and to compare toxic potency of BPF, BFDGE, and two of BPF metabolites using in vitro methods. BPF did not induce any genic mutation in bacteria when the Ames test was performed according to the OECD guideline. In contrast, using Human cell lines and Comet assay, we demonstrated that BPF and Bisphenol F Diglycidyl Ether (BFDGE) were effective on HepG2 cell DNA fragmentation at non-cytotoxic concentrations. DHB was also positive but at higher concentrations, near its limit of solubility. Neither BPF,…
Utilisation of pectin coating to enhance spray-dry stability of pea protein-stabilised oil-in-water emulsions
2010
International audience; In this study, development of pea (Pisum sativum) protein stabilised dry and reconstituted emulsions is presented. Dry emulsions were prepared by spray-drying liquid emulsions in a laboratory spray-dryer. The effect of drying on the physical stability of oil-in-water emulsions containing pea protein-coated and pea protein/pectin-coated oil droplets has been studied. Oil-in-water emulsions (5 wt.% Miglyol 812 N, 0.25 wt.% pea protein, 11% maltodextrin, pH 2.4) were prepared that contained 0 (primary emulsion) or 0.2 wt.% pectin (secondary emulsion). The emulsions were then subjected to spray-drying and reconstitution (pH 2.4). The stability of the emulsions to dry pro…
Deficit irrigation does not affect alternate bearing of ‘Valencia’ orange trees
2018
This study evaluated the effect of long-term partial rootzone drying and continuous deficit irrigation on the degree of alternate bearing of adult ‘Valencia’ orange trees grown in northern Sicily. Three irrigation strategies were imposed in summer from 2007 to 2011: irrigation with volumes corresponding to 100% of crop evapotranspiration applied to entire rootzone (CI), partial rootzone drying (PRD) with 50% of CI water applied to one alternated side of the rootzone, and continuous deficit irrigation (CDI) with 50% of CI water applied to both sides of the rootzone. The experiment was conducted on 48 adult orange trees arranged according to a randomized block design. Weather and soil paramet…
Evaluating the microscopic effect of brushing stone tools as a cleaning procedure
2020
Cleaning stone tool surfaces is a common procedure in lithic studies. The first step widely applied at any archeological site (and/or at field laboratories) is the gross removal of sediment from the surfaces of artifacts. Lithic surface alterations due to mechanical action applied in wet or dry cleaning regimes have never been examined at a microscopic scale. This could have important implications in traceology, as any modern surface modifications inflicted on archeological artifacts might compromise their functional interpretations. The current trend toward quantification of use-wear traces makes the testing even more important, as even slight, apparently invisible surface alterations migh…
Transformation of raw ewes' milk applying “Grana” type pressed cheese technology: development of extra-hard “Gran Ovino” cheese
2019
Abstract This work was carried out to pursue a double objective: to improve the hygienic safety of cheeses produced from raw ewes' milk; and to produce a new typology of raw ewes' milk through the application of “Grana” technology for which the name “Gran Ovino” was chosen. With this in mind, raw milk from an individual farm was transformed under controlled conditions at a dairy pilot plant. The production technology included the partial skimming of the evening and morning milk mixture by cream surfacing and the addition of a natural whey starter cultures (NWSC) prepared with four selected Streptococcus thermophilus strains (PON6, PON244, PON261 e PON413). Ten microbial groups were investig…
Improvement of Resveratrol Permeation through Sublingual Mucosa: Chemical Permeation Enhancers versus Spray Drying Technique to Obtain Fast-Disintegr…
2021
Resveratrol (RSV) is a natural polyphenol with several interesting broad-spectrum pharmacological properties. However, it is characterized by poor oral bioavailability, extensive first-pass effect metabolism and low stability. Indeed, RSV could benefit from the advantage of the sublingual route of administration. In this view, RSV attitudes to crossing the porcine sublingual mucosa were evaluated and promoted both by six different chemical permeation enhancers (CPEs) as well as by preparing four innovative fast-disintegrating sublingual mini-tablets by spray drying followed by direct compression. Since RSV by itself exhibits a low permeation aptitude, this could be significantly enhanced by…
Hydrogels for potential colon drug release by thiol-ene conjugate addition of a new inulin derivative.
2008
Inulin was chosen as a starting polymer for biocompatible, pH-sensitive and biodegradable hydrogels. Three INUDVSA-TT hydrogels were obtained by crosslinking inulin derivatives with trimethylolpropane tris(3-mercaptopropionate) under varying conditions. The resulting hydrogels were cell compatible, as demonstrated by MTS and trypan blue exclusion assays acting on Caco-2 cells, and were biodegraded by inulinase and esterase, thus suggesting their use as colonic drug delivery systems. 2-Methoxyestradiol, an anti-cancer drug, was soaked in INUDVSA-TT hydrogels and its in vitro release and apoptotic effect on Caco-2 cells were evaluated.
Volume change behaviour of a compacted scaly clay during cyclic suction changes
2010
The research presented in this paper focuses on the investigation and modelling of the volume change response of compacted tectonised clay samples subjected to several wetting and drying cycles in controlled-suction oedometers. Oedometer tests were carried out under different values of constant vertical net stress, and wetting and drying cycles were performed varying applied matric suction between 10 and 800 kPa. The investigation was complemented with a study of the material microstructure to support the interpretation of the overall mechanical response. At a microscopic level, the material is characterized by different types of particle assemblages, scales, and clay aggregates. One of th…
Fast synthesis of single-phased 110 K bismuth superconductor by freeze-drying of acetic precursors. Kinetic role of calcium and copper oxides
1993
Abstract Although the usefulness of solution procedures to improve the synthesis of bismuth HTSC has been argued, an adequate control of the procedural variables has allowed us to isolate the 110 K phase as the only superconducting phase after such a short sintering time as 22 h. The precursors used have been freeze-dried powders resulting from acetic solutions containing excess Ca and Cu. TGA-DTA and XRD experiments have been performed to follow the subsequent solid-state reaction processes. The initial formation of a mixed bismuth-lead oxoacetate, as intermediate to (Bi 1− y Pb y ) 2 CuO 4 , and the presence of an excess of Ca 2 CuO 3 in the last reaction step are two of the main factors …