Search results for "DUP"
showing 10 items of 499 documents
Crystallization and preliminary X-ray studies of mouse centrin1.
2005
The expression, purification, crystallization and preliminary X-ray diffraction studies of mouse centrin1 are reported. Centrins belong to a family of Ca{sup 2+}-binding EF-hand proteins that play a fundamental role in centrosome duplication and the function of cilia. To shed light on the structure–function relationship of these proteins, mouse centrin1 has been crystallized. The mouse centrin1 has been expressed in Escherichia coli as a GST-centrin fusion protein containing a thrombin protease cleavage site between the fusion partners. Two constructs with different linking-sequence lengths were expressed and purified. Thrombin cleavage yielded functional centrin1 and N-terminally extended …
Approaches for a Sustainable Use of the Bioactive Potential in Sponges: Analysis of Gene Clusters, Differential Display of mRNA and DNA Chips
2003
In recent years, analyses of the genome organization of marine sponges have begun that have led to the elucidation of selected genes and gene arrangements that exist in gene clusters (e.g. the receptor tyrosine kinase cluster and the allograft inflammatory factor cluster). Most of these studies were performed with the demosponge Suberites domuncula; but Geodia cydonium (Demospongiae), Aphrocallistes vastus (Hexactinellida) and Sycon raphanus (Calcarea) were also investigated. Both S. domuncula and G. cydonium possess a surprisingly large genome of approximately 1.7 pg DNA per haploid set. Taking the high gene density in these sponges into account and considering that predominantly single-co…
G1-Blend between a Differentiable Superquadric of Revolution and a Plane or a Sphere Using Dupin Cyclides
2008
In this article, we present a method to perform G1-continuous blends between a differentiable superquadric of revolution and a plane or a sphere using Dupin cyclides. These blends are patches delimited by four lines of curvature. They allow to avoid parameterization problems that may occur when parametric surfaces are used. Rational quadratic Bezier curves are used to approximate the principal circles of the Dupin cyclide blends and thus a complex 3D problem is now reduced to a simpler 2D problem. We present the necessary conditions to be satisfied to create the blending patches and illustrate our approach by a number of superellipsoid/plane and superellipsoid/sphere blending examples.
Blending Planes and Canal Surfaces Using Dupin Cyclides
2011
We develop two different new algorithms of G1-blending between planes and canal surfaces using Dupin cyclides. It is a generalization of existing algorithms that blend revolution surfaces and planes using a plane called construction plane. Spatial constraints were necessary to do that. Our work consist in building three spheres to determine the Dupin cyclide of the blending. The first algorithm is based on one of the definitions of Dupin cyclides taking into account three spheres of the same family enveloping the cyclide. The second one uses only geometric properties of Dupin cyclide. The blending is fixed by a circle of curvature onto the canal surface. Thanks to this one, we can determine…
Subdivisions of Ring Dupin Cyclides Using Bézier Curves with Mass Points
2021
Dupin cyclides are algebraic surfaces introduced for the first time in 1822 by the French mathematician Pierre-Charles Dupin. A Dupin cyclide can be defined as the envelope of a one-parameter family of oriented spheres, in two different ways. R. Martin is the first author who thought to use these surfaces in CAD/CAM and geometric modeling. The Minkowski-Lorentz space is a generalization of the space-time used in Einstein’s theory, equipped of the non-degenerate indefinite quadratic form $$Q_{M} ( \vec{u} ) = x^{2} + y^{2} + z^{2} - c^{2} t^{2}$$ where (x, y, z) are the spacial components of the vector $$ \vec{u}$$ and t is the time component of $$ \vec{u}$$ and c is the constant of the spee…
Intraoperative vascular assessment for estimation of risk in primary closure of omphalocele and gastroschisis.
2013
Surgical repair of anterior abdominal wall defects is often complicated by a discrepancy between the eviscerated organs and the intra-abdominal space available. Primary closure of the abdominal wall may result in increased intra-abdominal pressure and consecutive circulatory impairment. We report the results of a retrospective and consecutive prospective study evaluating the influence of different witameters on mortality and morbidity in children with gastroschitis and omphalocele. Both studies demonstrated that real-time sonographic investigations, intraoperative Doppler duplex sonography, and colour-coded Doppler sonography provide the oppurtunity to collect objective intraoperative data.…
Promoter and exon–intron structure of the protein kinase C gene from the marine sponge Geodia cydonium: evolutionary considerations and promoter acti…
1999
Abstract We report the gene structure of a key signaling molecule from a marine sponge, Geodia cydonium. The selected gene, which codes for a classical protein kinase C (cPKC), comprises 13 exons and 12 introns; the introns are, in contrast to those found in cPKC from higher Metazoa, small in size ranging from 93 nt to 359 nt. The complete gene has a length of 4229 nt and contains exons which encode the characteristic putative regulatory and catalytic domains of metazoan cPKCs. While in the regulatory domain only one intron is in phase 0, in the catalytic domain most introns are phase 0 introns, suggesting that the latter only rarely undergo module duplication. The 5′-flanking sequence of t…
Focal DNA Copy Number Changes in Neuroblastoma Target MYCN Regulated Genes
2013
Neuroblastoma is an embryonic tumor arising from immature sympathetic nervous system cells. Recurrent genomic alterations include MYCN and ALK amplification as well as recurrent patterns of gains and losses of whole or large partial chromosome segments. A recent whole genome sequencing effort yielded no frequently recurring mutations in genes other than those affecting ALK. However, the study further stresses the importance of DNA copy number alterations in this disease, in particular for genes implicated in neuritogenesis. Here we provide additional evidence for the importance of focal DNA copy number gains and losses, which are predominantly observed in MYCN amplified tumors. A focal 5 kb…
Artificial Intelligence + Distributed Systems = Agents
2009
The connection with Wirth’s book goes beyond the title, albeit confining the area to modern Artificial Intelligence (AI). Whereas thirty years ago, to devise effective programs, it became necessary to enhance the classical algorithmic framework with approaches applied to limited and focused subdomains, in the context of broad-band technology and semantic web, applications - running in open, heterogeneous, dynamic and uncertain environments-current paradigms are not enough, because of the shift from programs to processes. Beside the structure as position paper, to give more weight to some basic assertions, results of recent research are abridged and commented upon in line with new paradigms.…
A grid ant colony algorithm for the orienteering problem
2005
In this paper we propose a distributed ant colony algorithm to solve large scale orienteering problem instances. Our approach is based on a multi-colony strategy where each colony works in an independent portion (cluster) in the original graph. This results in no need for communicating pheromones information among colonies and in increasing speedup. We have implemented our algorithm as a .NET Web services infrastructure following a grid computing philosophy and we provide some promising experimental results to show the feasibility and effectiveness of our approach