Search results for "Dehydration-rehydration"

showing 2 items of 2 documents

Anhydrobiosis: Inside yeast cells

2018

International audience; Under natural conditions yeast cells as well as other microorganisms are regularly subjected to the influence of severe drought, which leads to their serious dehydration. The dry seasons are then changed by rains and there is a restoration of normal water potential inside the cells. To survive such seasonal changes a lot of vegetative microbial cells, which belong to various genera and species, may be able to enter into a state of anhydrobiosis, in which their metabolism is temporarily and reversibly suspended or delayed. This evolutionarily developed adaptation to extreme conditions of the environment is widely used for practical goals - for conservation of microorg…

0106 biological scienceslipid-phaseCell Survivaldesiccation toleranceMicroorganismBiophysicsBioengineeringSaccharomyces cerevisiaeBiology01 natural sciencesApplied Microbiology and BiotechnologyDehydration-rehydrationDesiccation tolerance03 medical and health scienceswine yeastIntracellular protective reactions010608 biotechnology[SDV.IDA]Life Sciences [q-bio]/Food engineeringOrganelle[SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineeringwater replacement hypothesisLaboratorium voor PlantenfysiologieDesiccationCryptobiosismembrane phase-transitions030304 developmental biology0303 health sciencesDehydrationWaterendoplasmic-reticulumplasma-membraneAnhydrobiosisYeastYeastDehydration–rehydrationYeast in winemaking[SDV.MP]Life Sciences [q-bio]/Microbiology and ParasitologyBiofysicaCellular MicroenvironmentIntracellular changesBiochemistryglass-transitioncandida-utilis cellsEPSAdaptationDesiccationsaccharomyces-cerevisiae cellsLaboratory of Plant PhysiologyBiotechnologyBiotechnology Advances
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Activity of the α-glucoside transporter Agt1 in Saccharomyces cerevisiae cells during dehydration-rehydration events

2018

Microbial cells can enter a state of anhydrobiosis under desiccating conditions. One of the main determinants of viability during dehydration-rehydration cycles is structural integrity of the plasma membrane. Whereas much is known about phase transitions of the lipid bilayer, there is a paucity of information on changes in activity of plasma membrane proteins during dehydration-rehydration events. We selected the α-glucoside transporter Agt1 to gain insights into stress mechanisms/responses and ecophysiology during anhydrobiosis. As intracellular water content of S. cerevisiae strain 14 (a strain with moderate tolerance to dehydration-rehydration) was reduced to 1.5 g water/g dry weight, th…

0301 basic medicineSaccharomyces cerevisiae ProteinsMonosaccharide Transport ProteinsEvolution030106 microbiologySaccharomyces cerevisiaeSaccharomyces cerevisiaeDehydration-rehydration03 medical and health sciencesGlucosidesBehavior and Systematicsα-Glucoside transporterMembrane proteinsGeneticsViability assayDesiccationLipid bilayerEcology Evolution Behavior and SystematicsMicrobial ViabilitySymportersbiologyStrain (chemistry)EcologyCell MembraneBiological TransportTransporterbiology.organism_classificationAnhydrobiosisYeastYeast030104 developmental biologyInfectious DiseasesBiochemistryMembrane proteinAnhydrobiosis; Dehydration-rehydration; Membrane proteins; Yeast; α-Glucoside transporter; Ecology Evolution Behavior and Systematics; Genetics; Infectious DiseasesIntracellular
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