Search results for "Detection"
showing 10 items of 2543 documents
A class-selective immunoassay for simultaneous analysis of anilinopyrimidine fungicides using a rationally designed hapten
2017
he development of multianalyte immunoassays constitutes a main research issue in the field of bioanalytical techniques. In the present study, class-specific antibodies against the three members of the anilinopyrimidine family of fungicides (pyrimethanil, cyprodinil and mepanipyrim) were raised by using a bioconjugate of a rationally designed hapten [5-(6-methyl-2-(phenylamino)pyrimidin-4-yl)pentanoic acid]. Highly sensitive immunoassays were developed for the generic determination of these compounds, using the competitive enzyme-linked immunosorbent assay (ELISA). Particularly, a direct antibody-coated competitive ELISA afforded identical sensitivity for the three anilinopyrimidines, with I…
Application of a low transition temperature mixture for the dispersive liquid–liquid microextraction of illicit drugs from urine samples
2021
© 2021 by the authors.
Combined heterologies for monoclonal antibody-based immunoanalysis of fluxapyroxad
2018
Nowadays, instrumental methodologies and rapid bioanalytical techniques complement each other for the analysis of toxic chemical compounds. Fluxapyroxad was commercialized a few years ago as a fungicide and today it is being used worldwide to control a variety of pests. In the present study, the development of monoclonal antibody-based immunochemical methods for the analysis of this chemical in food samples was evaluated for the first time. Novel haptens were synthesized and protein bioconjugates were prepared. High-affinity and specific monoclonal antibodies to fluxapyroxad were generated from two haptens with alternative linker tethering sites. Haptens with linker site heterology and a st…
Combination of supported liquid membrane and solid-phase extraction for sample pretreatment of triazine herbicides in juice prior to capillary electr…
2002
A possibility of a combination of supported liquid membrane (SLM) and solid-phase extraction (SPE) for the determination of atrazine at microgram level in different type of fruit juices is presented. In comparison to SPE extraction from juice samples, the application of SLM-SPE enrichment provides much cleaner extracts and the possibility of lowering the limit of detection as low as 30 microg/l. However, it was also shown that by appropriate manipulation of SLM extraction conditions mainly flow-rate of donor phase and volume ratio between donor and acceptor phase, the level of detection can be further decreased to 10 microg/l. The results suggest that the application of SLM extraction prior…
A solid device based on doped hybrid composites for controlling the dosage of the biocide N-(3-aminopropyl)-N-dodecyl-1,3-propanediamine in industria…
2015
A colorimetric composite device is proposed to determine the widely used biocide N-(3-aminopropyl)-N-dodecyl-1,3-propanediamine (ADP).This sensing device is based on a film of 1,2-Naphthoquinone-4-sulfonate (NQS) embedded into polydimethylsiloxane-tetraethylortosilicate-SiO2 nanoparticles composite (PDMS-TEOS-SiO2NPs). Semiquantitative analysis can be performed by visual inspection. Digitalized image or diffuse reflectance (DR) measurements can be carried out for quantitative analysis. Satisfactory detection limit (0.018%, w/v) and relative standard deviations <12% were achieved. The proposed device has been applied for the determination of ADP in detergent industrial formulations with reco…
A sustainable on-line CapLC method for quantifying antifouling agents like irgarol-1051 and diuron in water samples: Estimation of the carbon footpri…
2016
In this work, in-tube solid phase microextraction (in-tube SPME) coupled to capillary LC (CapLC) with diode array detection has been reported, for on-line extraction and enrichment of booster biocides (irgarol-1051 and diuron) included in Water Frame Directive 2013/39/UE (WFD). The analytical performance has been successfully demonstrated. Furthermore, in the present work, the environmental friendliness of the procedure has been quantified by means of the implementation of the carbon footprint calculation of the analytical procedure and the comparison with other methodologies previously reported. Under the optimum conditions, the method presents good linearity over the range assayed, 0.05-1…
A miniaturized method for estimating di(2-ethylhexyl) phthalate in bivalves as bioindicators.
2012
This work has developed a miniaturized method based on matrix solid phase dispersion (MSPD), using C18 as dispersant and acetonitrile-water as eluting solvent, for the analysis of di(2-ethylhexyl) phthalate (DEHP) in biota samples by capillary liquid chromatography coupled to in-tube solid phase microextraction and diode array detection. Recovery studies showed that the combination of C18-Florisil® was optimal using low amount of samples (0.1 g) and with low volumes of acetonitrile-water (2.6 mL 1:3.25, v/v). The sample is processed in less than 30 min, no evaporation step is required. The proposed method was applied to the analysis of DEHP in mussels and of the coastal waters in which they…
Label-free piezoelectric biosensor for prognosis and diagnosis of Systemic Lupus Erythematosus
2017
[EN] An autoantigen piezoelectric sensor to quantify specific circulating autoantibodies in human serum is developed. The sensor consisted on a quartz crystal microbalance with dissipation monitoring (QCM-D) where TRIM21 and TROVE2 autoantigens were covalently immobilized, allowing the selective determination of autoantibodies for diagnosis and prognosis of Systemic Lupus Erythematosus (SLE). The sensitivity of the biosensor, measured as IC50 value, was 1.51 U/mL and 0.32 U/mL, for anti-TRIM21 and anti-TROVE2 circulating autoantibodies, respectively. The sensor is also able to establish a structural interaction fingerprint pattern or profile of circulating autoantibodies, what allows scorin…
Local Directional Multi Radius Binary Pattern
2018
Face recognition becomes an important task performed routinely in our daily lives. This application is encouraged by the wide availability of powerful and low-cost desktop and embedded computing systems, while the need comes from the integration in too much real world systems including biometric authentication, surveillance, human-computer interaction, and multimedia management. This article proposes a new variant of LBP descriptor referred as Local Directional Multi Radius Binary Pattern (LDMRBP) as a robust and effective face descriptor. The proposed LDMRBP operator is built using new neighborhood topology and new pattern encoding scheme. The adopted face recognition system consists of th…
Analysis of emerging contaminants and nanomaterials in plant materials following uptake from soils
2017
The reuse of treated wastewater or wastewater-impacted river water is growing, particularly in arid areas, due to the climate change. As well, the disposal of sludges, biosolids or biochar as soil amendments to improve agricultural output is widely implemented in the EU. These practices can be a source of emerging contaminants and engineering nanomaterials (ENMs) to the environment and condition their incorporation to food chain that could affect human beings. This review covers current analytical techniques, instruments and methodologies used to determine emerging contaminants and ENMs from irrigation water and/or soil amendments in plants. Trends regarding sample treatment, separation and…