Search results for "Differentiation"
showing 10 items of 1605 documents
The Epithelial Mesenchymal Transition Process in Wilms Tumor
2011
Background Until now, only a few mouse-transplanted human tumors or experimental Wilms tumor (WT) cell lines have been described. The aim of this study was to show the biological behavior, including histology, immunohistochemistry (IHC), and molecular biology, of a WT including the original tumor and metastasis transferred into nude mice and followed for successive generations in xenografts. Methods A WT metastasis was xenotransplanted into nude mice and the mice was monitored for 7 passages over a period of 29 months; the original neoplasm was comparatively studied. The morphology was evaluated by optical and electron microscopy. The protein expression was analyzed by immunohistochemistry …
The gap junctional intercellular communication is no prerequisite for the stabilization of xenobiotic metabolizing enzyme activities in primary rat l…
1995
In primary monocultures of adult rat liver parenchymal cells (PC), the activities of the xenobiotic metabolizing enzymes microsomal epoxide hydrolase (mEHb), soluble epoxide hydrolase (sEH), glutathione S-transferases (GST), and phenolsulfotransferase (ST) were reduced after 7 d to values below 33% of the initial activities. Furthermore, the gap junctional intercellular communication (GJIC), measured after microinjection by dye transfer, decreased from 90% on Day 1 to undetectable values after 5 d in monoculture. Co-culture of PC with nonparenchymal rat liver epithelial cells (NEC) increased (98% on Day 1) and stabilized (82% on Day 7) the homotypic GJIC of PC. Additionally, most of the mea…
Temperature regulating floral bud differentiation in loquat (Eriobotrya japonica Lindl.). Hormonal and genetic aspects
2017
In loquat, apex of a current shoot changes from vegetative to reproductive stage during summer, i.e. under high temperature conditions. Indeed, just before floral bud differentiation, a decline in the growth rate due to high temperature takes place. The aim of this work is to study the role of this 'summer rest period' on the apex transition from vegetative to reproductive stage. For this purpose 1) sprouting of secondary shoots was promoted at different times, removing the main shoot, before, during and after floral bud differentiation occurred and 2) groups of trees were shifted to a greenhouse under average maximum temperature not exceeding 25 ° C during different periods from June to Oc…
AN IL-6/IL-6 SOLUBLE RECEPTOR (IL-6R) HYBRID PROTEIN (H-IL-6) INDUCES EPO-INDEPENDENT ERYTHROID DIFFERENTIATION IN HUMAN CD34+CELLS
2000
H-IL-6 is a hybrid protein constructed to contain IL-6 and its soluble receptor linked by a flexible peptide chain. Here we show that H-IL-6 strongly enhances proliferation of human CD34(+)cells in serum-free liquid culture, and that the majority of the cells generated belong to the erythroid lineage, being positive for the marker Glycophorin A. Conversely, H-IL-6 does not increase the number of myeloid, CD13-positive cells. Comparable effects are observed on progenitors from cord blood and adult peripheral blood. Therefore, H-IL-6 triggers an erythroid-inducing signal in haematopoietic progenitor cells, independently from erythropoietin (EPO).
A role for caspases in the differentiation of erythroid cells and macrophages
2007
Several cysteine proteases of the caspase family play a central role in many forms of cell death by apoptosis. Other enzymes of the family are involved in cytokine maturation along inflammatory response. In recent years, several caspases involved in cell death were shown to play a role in other cellular processes such as proliferation and differentiation. In the present review, we summarize the current knowledge of the role of caspases in the differentiation of erythroid cells and macrophages. Based on these two examples, we show that the nature of involved enzymes, the pathways leading to their activation in response to specific growth factors, and the specificity of the target proteins th…
Introducing the Study of Nordic Cooperation
2020
To rediscover Nordic cooperation this article develops a ‘conceptual grammar’ that provides general theoretical ‘images’ of cooperation that are systematically applied. Being supplementary analytical constructs, moreover, these images capture great variety and differentiation in Nordic cooperation. Next, this article provides a review of two sets of literature that are of particular relevance to this thematic issue. The first is a broader literature on European integration. The second is studies of Nordic cooperation. The article closes with an overview of the contributions to this thematic issue.
Between Differentiation and (Dis)Integration – Theoretical Explanations of a Post-Brexit European Union
2021
The authors of this paper provide a critical analysis of the most prominent theoretical vehicles employed in studying differentiated integration in contemporary, post-Brexit Europe. They discuss the descriptive, explanatory, and interpretative potential of the selected theoretical approaches that are applied at the intersection of disintegration and European differentiation discourse. “The holy grail” of the theorising of the dynamic (and accelerating) processes of (dis)integration and differentiation remains undiscovered. Nevertheless, a constant search for theoretical explanation is needed in the in-depth analyses of the current state of the European Union.
Mixed support for an alignment between phenotypic plasticity and genetic differentiation in damselfly wing shape
2023
The relationship between genetic differentiation and phenotypic plasticity can provide information on whether plasticity generally facilitates or hinders adaptation to environmental change. Here, we studied wing shape variation in a damselfly (Lestes sponsa) across a latitudinal gradient in Europe that differed in time constraints mediated by photoperiod and temperature. We reared damselflies from northern and southern populations in the laboratory using a reciprocal transplant experiment that simulated time-constrained (i.e. northern) and unconstrained (southern) photoperiods and temperatures. After emergence, adult wing shape was analysed using geometric morphometrics. Wings from individu…
The Competiveness of the Spanish Regions
2009
Competitiveness is a widely popular concept, which has saved in practise, to denote efficient economic behaviour. The wide dissemination of the competitiveness ranking of countries published annually by the World Economic Forum of Davos (Switzerland), or the fact that the European Union defines some of the global problems, which affect the countries of the EU in terms of the competitiveness gap, have footered the use of this expression in the media. The use of the term to denominate some of the objectives of the European Regional policy has also contributed to extending the use of the expression, as well as the work of prestigious consultants, such as Michael Porter, in influential works, s…