Search results for "Digest"
showing 8 items of 3038 documents
Functional properties of a monoclonal antibody inhibiting the hepatitis C virus RNA-dependent RNA polymerase.
2001
The hepatitis C virus (HCV) RNA-dependent RNA polymerase (RdRp), represented by nonstructural protein 5B (NS5B), has recently emerged as a promising target for antiviral intervention. Here, we describe the isolation, functional characterization, and molecular cloning of a monoclonal antibody (mAb) inhibiting the HCV RdRp. This mAb, designated 5B-12B7, binds with high affinity to a conformational epitope in the palm subdomain of the HCV RdRp and recognizes native NS5B expressed in the context of the entire HCV polyprotein or subgenomic replicons. Complete inhibition of RdRp activity in vitro was observed at equimolar concentrations of NS5B and mAb 5B-12B7, whereas RdRp activities of classica…
In vitro studies on the activation of the hepatitis C virus NS3 proteinase by the NS4A cofactor.
1996
AbstractProteolytic processing of the nonstructural proteins of the hepatitis C virus (HCV) is mediated by two viral proteinases: the NS2-3 proteinase cleaving at the NS2/3 junction and the NS3 serine-type proteinase responsible for processing at the NS3/4A, NS4A/B, NS4B/5A, and NS5A/B sites. Activity of the NS3 proteinase is modulated by NS4A. In the absence of this cofactor processing at the NS3-dependent sites does not occur or, in the case of the NS5A/B junction, is poor but increased when NS4A is present. Although recent studies demonstrated that proteinase activation requires direct interaction between NS3 and NS4A, the mechanism by which NS4A exerts the activation function is not kno…
Effect of temperature on anaerobic treatment of black water in UASB-septic tank systems
2007
The effect of northern European seasonal temperature changes and low temperature on the performance of upflow anaerobic sludge blanket (UASB)-septic tanks treating black water was studied. Three UASB-septic tanks were monitored with different operational parameters and at different temperatures. The results indicated the feasibility of the UASB-septic tank for (pre)treatment of black water at low temperatures with respect to removal of suspended solids and dissolved organic material. Inoculum sludge had little effect on COD(ss) removal, though in the start-up phase some poorly adapted inoculum disintegrated and washed out, thus requiring consideration when designing the process. Removal of …
Pleasure or health: Do children have to choose?
2017
International audience; Pleasure from eating is known to be a strong predictor of children’s food choices. Yet, children’s food preferences generally do not align with dietary recommendations, children rate energy-dense foods as the most liked and vegetables the least liked. In order to incite children to adopt a healthy diet, parents, caregivers, and national campaigns put forward nutritional arguments. However, there is limited research regarding the impact of the level of nutritional concern on children’s food choices, especially compared to pleasure-oriented goals. This talk is an overview of the research work I have conducted during my PhD, and explores the relationships between childr…
Low-level environmental metal pollution is associated with altered gut microbiota of a wild rodent, the bank vole (Myodes glareolus)
2021
Mining and related industries are a major source of metal pollution. In contrast to the well-studied effects of ex-posure to metals on animal physiology and health, the impacts of environmental metal pollution on the gut mi-crobiota of wild animals are virtually unknown. As the gut microbiota is a key component of host health, it is important to understand whether metal pollution can alter wild animal gut microbiota composition. Using a combination of 16S rRNA amplicon sequencing and quantification of metal levels in kidneys, we assessed whether multi-metal exposure (the sum of normalized levels of fifteen metals) was associated with changes in gut microbiota of wild bank voles (Myodes glar…
Consumption of ultra-processed foods : an assessment of the literature on determinants of ultra-processed food consumption and an investigation of th…
2016
Masteroppgave folkehelsevitenskap - Universitetet i Agder 2016 Background Highly processed foods have been classified as ultra-processed, and consumption of such foodstuffs have expanded rapidly over the last decades. Ultra-processed foods are characterized as being accessible, attractive, palatable and often time-saving. An excess intake of ultra-processed foods has been associated with increased risk of e.g. overweight/obesity and diabetes type II. The aim of this master’s project was to investigate factors influencing consumption of ultra-processed foods. This resulted in one review paper on young adults, and one cross-sectional study where the association between time scarcity and ultra…
Development of Food Preferences and Appetite in the First Years
2016
International audience; Infants are born equipped to ingest nutrients, but have to learn how, what and how much to eat. This must occur early, because the mode of feeding evolves dramatically, from ‘tube’ feeding in utero to eating table foods with the family. Eating habits established during early years contribute to the development of subsequent eating habits. Therefore, it is fundamental to understand the most important early periods for the development of eating habits and the drivers of this development. Here we will focus on the first three years of postnatal life. Several characteristics of the eating experience contribute to drive infant’s eating and to shape preferences and energy …
Validation in Young Soccer Players of the Modified Version of the Harre Circuit Test: The Petrucci Ability Test
2021
The evaluation of soccer players’ physical fitness from youth onward is important for monitoring performance and planning training. While health-related factors present valid and reliable tests, the skill-related component should be studied in depth. An interesting test to evaluate the skill-related factors is the Harre circuit test (HTC); unfortunately, this test includes the somersault, an element not present in soccer. The aim of the present study is the validation of the Petrucci ability test (PAT), a variation of the HTC without the somersault for young soccer players. Children and adolescents (age range 10–13 years old) soccer players concluded the 20-m, the HTC and the PAT. To establ…