Search results for "Digital holographic microscopy"
showing 5 items of 55 documents
Physical compensation of phase curvature in digital holographic microscopy by use of programmable liquid lens.
2015
Quantitative phase measurements obtained with digital holographic microscopes are strongly dependent on the optical arrangement of the imaging system. The nontelecentric operation provides phase measurements affected by a parabolic phase factor and requires numerical postprocessing, which does not always remove all the perturbation. Accurate phase measurements are achieved by using the imaging system in telecentric mode. Unfortunately, this condition is not accomplished when a commercial microscope is used as the imaging system. In this paper, we present an approach for obtaining accurate phase measurements in nontelecentric imaging systems without the need for numerical postprocessing. The…
Superresolved common-path phase-shifting digital inline holographic microscopy using a spatial light modulator.
2012
Common-path phase-shifting lensless holographic microscopy has been recently proposed as a novel approach capable of high numerical aperture imaging in a lensless digital inline holographic microscopy layout [Opt. Lett.35, 3919 (2010)]. Here we present proof-of-concept validation for improving the resolution limit imposed by diffraction in such a setup. This is accomplished by shifting the phase lens displayed at the spatial light modulator, which moves the illumination point source to different off-axis positions. For each off-axis position, a set of inline phase-shifted holograms are recorded by the digital sensor and stored at the computer’s memory for later digital postprocessing. As a …
Quantitative Phase Imaging in Microscopy Using a Spatial Light Modulator
2010
In this chapter, we present a new method capable of recovery of the quantitative phase information of microscopic samples. Essentially, a spatial light modulator (SLM) and digital image processing are the basics to extract the sample’s phase distribution. The SLM produces a set of misfocused images of the input sample at the CCD plane by displaying a set of lenses with different power at the SLM device. The recorded images are then numerically processed to retrieve phase information. Computations are based on the wave propagation equation and lead to a complex amplitude image containing information of both amplitude and phase distributions of the input sample diffracted wave front. The prop…
Features extraction on complex images
2005
The accessibility of inexpensive and powerful computers has allowed true digital holography to be used for industrial inspection using microscopy. This technique allows the capture of a complex image (i.e., one containing magnitude and phase), and the reconstruction of the phase and magnitude information. Digital holograms give a new dimension to texture analysis, since the topology information can be used as an additional way to extract features. This new technique can be used to extend previous work on the image segmentation of patterned wafers for defect detection. The paper presents a comparison between the features obtained using Gabor filtering on complex images under illumination and…
Reconstruction Improvement in Integral Fourier Holography by Micro-Scanning Method
2015
Although integral holography has many promising advantages in the field of 3D imaging, the resolution of reconstructed holographic image is still limited by the insufficient information captured. To improve the reconstruction quality, an integral Fourier holographic imaging method based on micro-scanning of the micro-lens array is proposed in this paper. The micro-scanning of the micro-lens array can increase the sampling rate in spatial frequency domain and the information of the generated Fourier hologram, which will eventually eliminate the overlapping effect in the reconstructed 3D image. Experiments for different micro-scanning modes are carried out to verify the feasibility of the pro…