Search results for "Direct"

showing 10 items of 2669 documents

Identification of cyclin A as a molecular target of antinuclear antibodies (ANA) in hepatic and non-hepatic autoimmune diseases.

1996

Abstract Background/Aims: Antinuclear antibodies (ANA) are a diagnostic of various autoimmune diseases and also of autoimmune hepatitis type 1. The designation ANA describes a heterogeneous group of autoantibodies. In liver diseases, only a few nuclear target antigens have been molecularly identified and characterized. Cyclins play a central role in a cell cycle regulation, DNA transcription, and cell proliferation. Cyclin A was also identified as an integration site of the hepatitis B virus in a patient with hepatocellular carcinoma. In this study we identify cyclin A as a novel nuclear target protein of ANA. Methods: Sera of patients with autoimmune hepatitis (AIH) type 1 ( n =61), type 2…

AdultPathologymedicine.medical_specialtyAnti-nuclear antibodyCyclin ABlotting WesternImmunoblottingEnzyme-Linked Immunosorbent AssayAutoimmune hepatitisImmunofluorescenceAutoantigensAutoimmune DiseasesMixed connective tissue diseaseimmune system diseasesCyclinsMedicineHumansLupus Erythematosus Systemicskin and connective tissue diseasesFluorescent Antibody Technique IndirectAutoantibodiesAutoimmune diseaseHepatologymedicine.diagnostic_testbiologybusiness.industryLiver DiseasesAutoantibodyDNAMiddle Agedmedicine.diseaseRecombinant ProteinsAntibodies AntinuclearImmunologybiology.proteinAntibodybusinessBaculoviridaeJournal of hepatology
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Six novel mutations of the LDL receptor gene in FH kindred of Sicilian and Paraguayan descent

2006

Familial hypercholesterolemia (FH) is an autosomal dominant inherited disease caused by mutations in the gene coding for the low density lipoprotein receptor (LDL-R). It is characterized by a high concentration of low density lipoprotein (LDL), which frequently gives rise to premature coronary artery disease. We studied the probands of five FH Sicilian families with 'definite' FH and one proband of Paraguayan descent with homozygous FH who has been treated with an effective living-donor liver transplantation. In order to seek the molecular defect in these six families, we used direct sequencing to define the molecular defects of the LDL-R gene responsible for the disease. We described three…

AdultProbandhypercholesterolemia LDL receptor gene mutation analysis direct sequencing splicing living-donor transplantationSettore MED/09 - Medicina InternaDNA Mutational AnalysisDirect sequencingHypercholesterolemiaFamilial hypercholesterolemiaBiologyGene mutationSplicingmedicine.disease_causeFrameshift mutationHyperlipoproteinemia Type IIExonGeneticsmedicineHumansMissense mutationRNA MessengerChildSicilyCells CulturedLiving-donor transplantationLDL receptor geneGeneticsMutationIntronExonsGeneral MedicineMiddle Agedmedicine.diseaseLipidsMolecular biologyPedigreeDirect sequencing; Hypercholesterolemia; LDL receptor gene; Living-donor transplantation; Mutation analysis; SplicingMutation analysisReceptors LDLParaguayChild PreschoolMutationBiological Assay
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Mixed predictability and cross-validation to assess non-linear Granger causality in short cardiovascular variability series

2006

A method to evaluate the direction and strength of causal interactions in bivariate cardiovascular and cardiorespiratory series is presented. The method is based on quantifying self and mixed predictability of the two series using nearest-neighbour local linear approximation. It returns two causal coupling indexes measuring the relative improvement in predictability along direct and reverse directions, and a directionality index indicating the preferential direction of interaction. The method was implemented through a cross-validation approach that allowed quantification of directionality without constraining the embedding of the series, and fully exploited the available data to maximise th…

AdultStatistics as TopicBiomedical EngineeringInferenceBlood PressureHealth InformaticsBivariate analysisDirectionalityCross-validationGranger causalityHeart RateStatisticsEconometricsHumansComputer SimulationPredictabilityMathematicsSeries (mathematics)Models CardiovascularNonlinear systemNonlinear DynamicsData Interpretation StatisticalShort-term cardiovascular variabilityRespiratory MechanicsRegression AnalysisFemaleNon-linear predictionLinear approximationAlgorithmsBiomedizinische Technik/Biomedical Engineering
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Detection of IgM antibodies specific for measles virus by capture and indirect enzyme immunoassays.

1995

Summary During a measles outbreak, 112 serum specimens from 88 hospitalized patients were received in our laboratory for investigation of a morbilliform rash. These specimens (88 acute- and 24 convalescent-phase) were tested for the presence of measles-specific IgM antibodies by a capture EIA (enzyme immunoassay) using peroxidase-conjugated measles virus antigens and by an indirect EIA. Commercially available indirect EIA kits for measles-specific IgM antibodies were also used and compared with our homemade EIAs. Specificity studies included a collection of serum specimens containing rheumatoid factor, antinuclear antibodies or IgM antibodies specific to other viruses, and sera from blood d…

AdultTime FactorsAnti-nuclear antibodyParamyxoviridaeAdolescentImmunologyEnzyme-Linked Immunosorbent AssayAntibodies ViralMeaslesSensitivity and SpecificitySerologyDisease OutbreaksMeasles virusImmunoenzyme TechniquesMorbillivirusAntibody SpecificityVirologyChlorocebus aethiopsmedicineAnimalsHumansChildVero Cellsbiologymedicine.diagnostic_testInfantbiology.organism_classificationmedicine.diseaseRashVirologyImmunoglobulin MEvaluation Studies as TopicMeasles virusImmunoassayChild PreschoolImmunologyReagent Kits Diagnosticmedicine.symptomCapture EIA IgM Indirect EIAMeaslesResearch in virology
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Direct and Indirect Effects of Function in Associated Variables Such as Depression and Severity on Pain Intensity in Women with Carpal Tunnel Syndrome

2015

Objective To determine the direct and indirect effects of function on clinical variables such as age, pain intensity, years of the disease, severity of symptoms, and depression in women with electrodiagnostic and clinical diagnosis of carpal tunnel syndrome (CTS). Design A cross-sectional study. Setting Patients from an urban hospital referred to a university clinic. Methods Two hundred and forty-four (n = 224) women with CTS were included. Demographic and clinical data, duration of symptoms, function, symptom's severity of the symptoms, pain intensity, and depression were self-reported collected. Correlation and path analysis with maximum likelihood estimation were conducted to assess the …

Adultmedicine.medical_specialtyClinical variablesMaximum likelihoodPainDiseaseComorbiditySensitivity and SpecificitySeverity of Illness IndexRisk FactorsPrevalenceMedicineHumansCarpal tunnel syndromeAgedPain MeasurementHand functionbusiness.industryDepressionDirect effectsReproducibility of ResultsGeneral MedicineRecovery of FunctionMiddle Agedmedicine.diseaseCarpal Tunnel SyndromeCausalityAnesthesiology and Pain MedicineCross-Sectional StudiesPsicobiologiaSpainClinical diagnosisPhysical therapyNeuralgiaWomen's HealthFemaleNeurology (clinical)Self ReportSymptom AssessmentbusinessUrban hospital
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Inducible nitric oxide synthase in skeletal muscle of patients with chronic heart failure

1998

Abstract Objectives. The expression and localization of inducible nitric oxide (NO) synthase (NOS II) was evaluated as a source of NO which has been shown to affect muscle contraction. Background. Advanced stages of chronic heart failure are associated with systemic activation of cytokines which have been shown to stimulate the expression of NOS II in various cell types, including myocytes. We hypothesized that systemic cytokine activation could lead to expression of NOS II in skeletal muscle of patients with chronic heart failure. Methods. Skeletal muscle specimens were obtained by percutaneous needle biopsy in six normal volunteers and eight patients with heart failure (New York Heart Ass…

Adultmedicine.medical_specialtyHeart diseaseGene ExpressionNitric Oxide Synthase Type IIPolymerase Chain ReactionNitric oxidechemistry.chemical_compoundInternal medicineGene expressionmedicineHumansMyocyteRNA MessengerMicroscopy ImmunoelectronMuscle SkeletalHeart Failurebiologybusiness.industrySkeletal muscleRNA-Directed DNA PolymeraseMiddle Agedmedicine.diseaseNitric oxide synthaseEndocrinologymedicine.anatomical_structurechemistryHeart failureChronic Diseasebiology.proteinNitric Oxide Synthasemedicine.symptombusinessCardiology and Cardiovascular MedicineMuscle contractionJournal of the American College of Cardiology
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Quantifying the contribution of arm postural tremor to the outcome of goal-directed pointing task by displacement measures.

2004

A method for quantifying the outcome of goal-directed postural pointing was presented and used for relating the tremor output to the oscillations of single arm landmarks. The displacement of reflective markers placed on shoulder, upper arm, forearm, and hand were measured by an optoelectronic motion capture system in nine subjects holding a laser penlight pointed at a target. The high signal-to-noise ratio of the measured displacement series (from 7:1 for shoulder marker to 30:1 for hand marker) demonstrated the feasibility of the proposed system to carry out tremor analysis. The track of the laser emission on the target, reconstructed from penlight displacements, was studied as the outcome…

Adultmedicine.medical_specialtyMovementPhysiological tremor; Motor control; Goal-directed pointing; Human movement analysis; Spectral analysisSpectral analysisMotion captureHuman movement analysisTask (project management)Spectral analysiPhysical medicine and rehabilitationForearmControl theoryMotor controlTremormedicineHumansDisplacement (orthopedic surgery)PhysicsAnalysis of VarianceNeuroscience (all)General NeuroscienceTrack (disk drive)Motor controlPostural tremorPhysiological tremormedicine.anatomical_structureHuman movement analysiArmLow-frequency oscillationPhotic StimulationPsychomotor PerformanceGoal-directed pointing
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The Respect of Recommendations Provided in an International Travelers' Medical Service: Far From the Cup to the Lips

2013

Equipe plutôt EA Pôle MERS CT3 EJ3; Background: It is not clearly known how frequently the recommendations given to travelers are followed, and what factors could encourage compliance with these recommended measures. Methods: Adults consulting at a Medical Department for International Travelers (International Travelers' Medical Services, ITMS) in October and November 2010 were asked to answer a questionnaire before their journey. They were also contacted for a post-travel telephone interview to determine whether they had followed the recommendations regarding vaccinations and malaria prevention, and the reasons for poor or noncompliance with these recommendations. Results A total of 353 tra…

Adultmedicine.medical_specialtyPediatricsMultivariate analysisprévention conseil[SDV]Life Sciences [q-bio]MEDLINEDirective CounselingChemopreventionAntimalarialsRisk FactorsSurveys and QuestionnairesHealth careOutcome Assessment Health CarePreventive Health ServicesMedicineTravel medicineHumans[SDV.BV]Life Sciences [q-bio]/Vegetal BiologyMedical prescriptionTravelMosquito Netsbusiness.industryVaccinationGeneral MedicineMiddle AgedMalariaTelephone interviewSocioeconomic FactorsFamily medicineChemoprophylaxis[SDE]Environmental SciencesMosquito netPatient ComplianceFemaleFrancebusinesshuman activitiesTravel Medicine
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How does a lifestyle intervention during pregnancy influence perceived barriers to leisure-time physical activity? The Norwegian fit for delivery stu…

2018

Abstract Background To develop effective health promotional and preventive prenatal programs, it is important to understand perceived barriers to leisure-time physical activity during pregnancy, including exercise and sport participation. The aims of the present study was 1) to assess the effect of prenatal lifestyle intervention on the perceived barrier to leisure-time physical activity during pregnancy and the first year after delivery and 2) identify the most important perceived barriers to leisure-time physical activity at multiple time points during and after pregnancy. Methods This secondary analysis was part of the Norwegian Fit for Delivery study, a combined lifestyle intervention e…

Adultmedicine.medical_specialtyTime FactorsReproductive medicineDirective CounselingHealth PromotionPrenatal carelcsh:Gynecology and obstetricslaw.inventionYoung Adult03 medical and health sciencesLeisure Activities0302 clinical medicineRandomized controlled triallawPregnancyIntervention (counseling)medicineHumans030212 general & internal medicineExerciseLife StyleFatiguelcsh:RG1-991Self-efficacyMotivationPregnancy030219 obstetrics & reproductive medicineNorwaybusiness.industryPhysical activitySupervised exerciseObstetrics and GynecologyPrenatal CareFearmedicine.diseaseSelf EfficacyDietPhysical therapyFemalebusinessBody mass indexPostpartum periodBarriersRCTResearch Article
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Life satisfaction, general well-being and costs of treatment for severe fear of childbirth in nulliparous women by psychoeducative group or conventio…

2015

Objective Fear of childbirth is a common reason for seeking cesarean section. It is important to consider outcomes and costs associated with alternative treatment and delivery mode. We compared well-being and costs of group psychoeducation and conventional care for fear of childbirth. Design Randomized controlled trial. Population A total of 371 nulliparous women scoring over the 95th centile in the Wijma Delivery Expectancy Questionnaire (W-DEQ) during the first trimester. Setting Finland, data from obstetrical patient records and questionnaires. Methods Randomization to group psychoeducation with relaxation (six sessions during pregnancy, one after childbirth, n = 131), or surveillance an…

Adultmedicine.medical_specialtygroup psychoeducationReferralCost effectivenessmedicine.medical_treatmentHealth StatusPopulationfear of childbirthPersonal SatisfactionRelaxation TherapyIndirect costsPrenatal EducationPregnancySurveys and QuestionnairesPsychoeducationMedicineChildbirthHumansMaternal Health Serviceseducationcost-effectivenesslife satisfactionMaternal Welfareta515Finlandeducation.field_of_studyPregnancycesarean sectionbusiness.industryObstetricsParturitionObstetrics and GynecologyGeneral MedicineFearHealth Care CostsDelivery modemedicine.diseaseta31233. Good healthParityPsychotherapy GroupQuality of LifeFemalebusinessActa obstetricia et gynecologica Scandinavica
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