Search results for "Disc"
showing 10 items of 19256 documents
Acceso al empleo del colectivo de personas transexuales de Valencia: retos y barreras a superar en la sociedad actual
2017
Spānijas, Francijas un Latvijas valsts vadītāju Ziemassvētku uzrunu retorikas analīze pandēmijas laikā
2022
Šis maģistra darbs ir veltīts Spānijas, Francijas un Latvijas Valsts vadītāju Ziemassvētku un Jaungada uzrunu retorikas analīzei pandēmijas laikā. Darbā izvirzītais mērķis ir izmantot klasisko retoriku, kas papildināta ar diskursīvās analīzes teoriju, lai analizētu sešas Ziemassvētku uzrunas un noskaidrotu, vai tajās ir redzamas vienotas tendences uzbūvē, leksikā un nolūkā. Darba gaitā mērķis ir sasniegts. Teorētiskās daļas pamatā ir retorikas klasiķu pieeja retorikas uzdevumu definēšanā, kā arī mūslaiku autoru pieeja diskursa analīzē, kur autore ir pētījusi kopsakarības. Praktiskajā daļā ir analizētas sešas uzrunas - trīs valstu vadītāju uzrunas 2020. gada nogalē un trīs - 2021. gada svētk…
La construcción de las identidades de la mujer contemporánea en la revista Cosmpolitan. Una aproximación multimodal de las ediciones de EEUU y España
2018
La presente tesis doctoral está centrada en el análisis de las identidades de la mujer contemporánea representadas en el discurso de las ediciones española y estadounidense de la revista Cosmopolitan. Esta investigación permite reflexionar sobre el concepto de identidad (Bauman, 2005, Lipovetsky, 2016), principalmente la identidad de la mujer en los contextos socioculturales de España y Estados Unidos (Butler, 1990; Giddens, 1995; Hinojosa, 2005). A través de dicha reflexión, también observamos los efectos de las formas de representación de los discursos mediáticos, dirigidos a la mujer, en la creación de significados y creencias compartidas en las comunidades de práctica dentro de la socie…
El concepto de nación en el constitucionalismo decimonónico español
2017
En este estudio se analiza el concepto de nación española por medio de los textos constitucionales decimonónicos y los debates que se desarrollaron entorno a su redacción en las sesiones plenarias de las Cortes. De este modo, se pretende arrojar luz sobre la comprensión del concepto de nación como idea no sólo metajurídica sino también jurídica. En esta tesis doctoral se examina la idea de nación desde una triple vertiente jurídica, convinando la Historia del Derecho, el Derecho Constitucional y la Filosofía del Derecho. De este modo, desde una perspectiva puramente jurídica, la nación se dilucida como un constructo surgido de la voluntad política, que a su vez se asienta y legitima mediant…
Performative Control and Rhetoric in Aotearoa New Zealand’s Response to COVID-19
2021
This article analyses how specific nodal points of performative control developed and consequently structured the discourse on Aotearoa New Zealand’s response to the COVID-19 pandemic. It identifies these points by adopting a rhetorical-performative approach to uncover three particular performances of control that articulated the pandemic in Aotearoa New Zealand, from the diagnosis of the first COVID-19 case in the country in February 2020 through to October 2020. This period of analysis covers the emergence, subsequent nationwide lockdown, elimination, and re-emergence of the virus. There are three distinct nodal points that unfold as key to the nation’s ability to control COVID-19: the he…
Cytotoxic necrotizing factor type 2 produced by virulent Escherichia coli modifies the small GTP-binding proteins Rho involved in assembly of actin s…
1994
Cytotoxic necrotizing factor type 2 (CNF2) produced by Escherichia coli strains isolated from intestinal and extraintestinal infections is a dermonecrotic toxin of 110 kDa. We cloned the CNF2 gene from a large plasmid carried by an Escherichia coli strain isolated from a lamb with septicemia. Hydropathy analysis of the deduced amino acid sequence revealed a largely hydrophilic protein with two potential hydrophobic transmembrane domains. The N-terminal half of CNF2 showed striking homology (27% identity and 80% conserved residues) to the N-terminal portion of Pasteurella multocida toxin. Methylamine protection experiments and immunofluorescence studies suggested that CNF2 enters the cytosol…
Targeting the mevalonate pathway for improved anticancer therapy.
2009
The mevalonate pathway is important for the generation of isoprene moieties thereby providing the basis for the biosynthesis of molecules required for maintaining membrane integrity, steroid production and cell respiration. Additionally, isoprene precursors are indispensable for the prenylation of regulatory proteins such as Ras and Ras-homologous (Rho) GTPases. These low molecular GTP-binding proteins play key roles in numerous signal transduction pathways stimulated upon activation of cell surface receptors by ligand binding. Thus, Ras/Rho proteins eventually regulate cell proliferation, tumor progression and cell death induced by anticancer therapeutics. Lipid modification of Ras/Rho pro…
Delivery of proteins into living cells by reversible membrane permeabilization with streptolysin-O
2001
The pore-forming toxin streptolysin O (SLO) can be used to reversibly permeabilize adherent and nonadherent cells, allowing delivery of molecules with up to 100 kDa mass to the cytosol. Using FITC-labeled albumin, 10 5 –10 6 molecules were estimated to be entrapped per cell. Repair of toxin lesions depended on Ca 2+ -calmodulin and on intact microtubules, but was not sensitive to actin disruption or to inhibition of protein synthesis. Resealed cells were viable for days and retained the capacity to endocytose and to proliferate. The active domains of large clostridial toxins were introduced into three different cell lines. The domains were derived from Clostridium difficile B-toxin and Clo…
A novel microtubule de-stabilizing complementarity-determining region C36L1 peptide displays antitumor activity against melanoma in vitro and in vivo
2015
AbstractShort peptide sequences from complementarity-determining regions (CDRs) of different immunoglobulins may exert anti-infective, immunomodulatory and antitumor activities regardless of the specificity of the original monoclonal antibody (mAb). In this sense, they resemble early molecules of innate immunity. C36L1 was identified as a bioactive light-chain CDR1 peptide by screening 19 conserved CDR sequences targeting murine B16F10-Nex2 melanoma. The 17-amino acid peptide is readily taken up by melanoma cells and acts on microtubules causing depolymerization, stress of the endoplasmic reticulum and intrinsic apoptosis. At low concentrations, C36L1 inhibited migration, invasion and proli…
Rho GTPases: Promising Cellular Targets for Novel Anticancer Drugs
2006
Ras-homologous (Rho) GTPases play a pivotal role in the regulation of numerous cellular functions associated with malignant transformation and metastasis. Rho GTPases are localized at membranes and become activated upon stimulation of cell surface receptors. In their GTP-bound (=active) state, Rho proteins bind to effector proteins, thereby triggering specific cellular responses. Members of the Rho family of small GTPases are key regulators of actin reorganization, cell motility, cell-cell and cell-extracellular matrix (ECM) adhesion as well as of cell cycle progression, gene expression and apoptosis. Each of these functions is of importance for the development and progression of cancer. Fu…