Search results for "Diseases."

showing 10 items of 20181 documents

Editorial: Thymic Epithelial Cells: New Insights Into the Essential Driving Force of T-Cell Differentiation.

2021

thymic stromal cellsT-LymphocytesImmunologyThymus GlandBiology03 medical and health sciences0302 clinical medicinethymusmedicineImmunology and AllergyAnimalsHumansImmunodeficiency030304 developmental biologycentral tolerance0303 health sciencesCell DifferentiationEpithelial CellsRC581-607medicine.diseaseCell biologyEditorialT cell differentiationthymic epithelial cellsCentral toleranceImmunologic diseases. Allergyimmunodeficiency030215 immunologyFrontiers in immunology
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Variants of human CLDN9 cause mild to profound hearing loss

2021

Hereditary deafness is clinically and genetically heterogeneous. We investigated deafness segregating as a recessive trait in two families. Audiological examinations revealed an asymmetric mild to profound hearing loss with childhood or adolescent onset. Exome sequencing of probands identified a homozygous c.475G>A;p.(Glu159Lys) variant of CLDN9 (NM_020982.4) in one family and a homozygous c.370_372dupATC;p.(Ile124dup) CLDN9 variant in an affected individual of a second family. Claudin 9 (CLDN9) is an integral membrane protein and constituent of epithelial bicellular tight junctions that form semi-permeable, paracellular barriers between inner ear perilymphatic and endolymphatic compartment…

tight junctionsAdolescentclaudin 9In situ hybridizationDeafnessBiologyArticleFrameshift mutationMiceotorhinolaryngologic diseasesGeneticsmedicineAnimalsHumansPakistanInner earNonsyndromic deafnessChildClaudinGenetics (clinical)Exome sequencingnonsyndromic deafnessTight junctionGenetic heterogeneityclaudin 9; exome sequencing; Morocco; nonsyndromic deafness; Pakistan; tight junctionsHomozygotemedicine.diseaseMolecular biologyPedigreeMoroccomedicine.anatomical_structureClaudinsMutationexome sequencingHeLa CellsHuman Mutation
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Development of polypeptide-based therapeutics for topical delivery

2019

Topical administration represents the main route to attain local therapeutic activity of bioactive agents in several organs, such as the skin or the heart by means of devices that enhance drug transport through the endothelium acting as a reservoir. The complex structure of the skin protects the human body against potentially harmful external agents; however, this protective mechanism inhibits the penetration of topically administering bioactive agents employed for the treatment of skin diseases. Unfortunately, many of the topical drugs currently used or under evaluation in clinical trials lack the appropriate physico-chemical characteristics required for delivery through the skin. However,…

tissue healingtopical deliveryintegumentary systempolymer-drug conjugatesskin diseasesUNESCO::QUÍMICApolymer therapeutics:QUÍMICA [UNESCO]
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Single-cell analysis of population context advances RNAi screening at multiple levels

2012

Isogenic cells in culture show strong variability, which arises from dynamic adaptations to the microenvironment of individual cells. Here we study the influence of the cell population context, which determines a single cell's microenvironment, in image‐based RNAi screens. We developed a comprehensive computational approach that employs Bayesian and multivariate methods at the single‐cell level. We applied these methods to 45 RNA interference screens of various sizes, including 7 druggable genome and 2 genome‐wide screens, analysing 17 different mammalian virus infections and four related cell physiological processes. Analysing cell‐based screens at this depth reveals widespread RNAi‐induce…

toImage ProcessingDruggabilityGenomeImage analysis0302 clinical medicineComputer-AssistedSX00 SystemsX.ch2604 Applied MathematicsSingle-cell analysisRNA interferenceModels2400 General Immunology and MicrobiologyImage Processing Computer-AssistedViralRNA Small Interfering0303 health scienceseducation.field_of_studyApplied MathematicsSystems BiologyGenomics10124 Institute of Molecular Life SciencesCell biologycell variabilityComputational Theory and MathematicsCellular MicroenvironmentVirus DiseasesVirusesRNA ViralRNA InterferenceSingle-Cell AnalysisGeneral Agricultural and Biological SciencesInformation SystemsSystems biologyVirus infectionPopulationContext (language use)Genomics1100 General Agricultural and Biological SciencesBiologySmall InterferingModels BiologicalGeneral Biochemistry Genetics and Molecular BiologySX08 LipidX03 medical and health sciencesViral ProteinsCell-to-cell variability; Image analysis; Population context; RNAi; Virus infection1300 General Biochemistry Genetics and Molecular BiologyHumansComputer Simulationeducation030304 developmental biologyGeneral Immunology and MicrobiologyCell-to-cell variabilityReproducibility of ResultsBayes TheoremcellBiologicalPopulation contextRNAi570 Life sciences; biologyRNA030217 neurology & neurosurgeryHeLa CellsMolecular Systems Biology
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Burnout syndrome assessment among Spanish oral surgery consultants: A two populations comparative pilot study.

2021

Background The professional Burnout Syndrome (BOS) or Burnout is considered a professional disease made up of three interrelated dimensions (emotional exhaustion, depersonalization and lack of personal fulfillment). BOS has been documented to most severely affect the healthcare professions, especially dentists. On the other hand, its appearance has been documented at an early age, during dental training. However, there are no studies that analyze its incidence in professionals dedicated to Oral Surgery and Implantology, determining the age of onset and related factors. Material and Methods The modified Maslach questionnaire was carried out anonymously among the professors and students of th…

tongue lesionsConsultantsallergiesResearcheducationOral Surgical ProceduresPilot Projects610 Medicine & healthfissured tonguesystemic diseasesmedicationsOtorhinolaryngologyChild PreschoolSurveys and QuestionnairesHumansrisk factorsSurgeryOral Surgery610 Medicine & healthGeneral DentistryBurnout ProfessionalUNESCO:CIENCIAS MÉDICAS
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Efectos del Sorafenib, un inhibidor tirosina quinasa, en el control del tono vascular.

2018

El carcinoma hepatocelular o hepatocarcinoma (HCC) es uno de los tumores sólidos vasculares más conocidos, representa uno de los mayores problemas de salud en todo el mundo, siendo una de las enfermedades más comunes del hígado (70-85%) y una de las mayores causas de mortalidad relacionadas con el cáncer, siendo la tercera causa más común de muerte por cáncer Varias rutas carcinogénicas se han identificado en el desarrollo y progresión del HCC, proporcionando nuevas dianas moleculares para el tratamiento del HCC. Algunos fármacos que afectan a estas rutas incluyen, entre otros, el receptor del factor de crecimiento endotelial vascular (VEGFR). El sorafenib es un fármaco sistémico aprobado p…

tono vascularinhibidor tirosina quinasaUNESCO::CIENCIAS MÉDICASUNESCO::CIENCIAS DE LA VIDAsorafenib:CIENCIAS MÉDICAS [UNESCO]digestive system diseases:CIENCIAS DE LA VIDA [UNESCO]
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Improving the Effect of Transcranial Alternating Current Stimulation (tACS): A Systematic Review

2021

With the development of electrical stimulation technology, traditional transcranial alternating current stimulation (tACS) technology has been found to have the drawback of not targeting a specific area accurately. Studies have shown that optimizing the number and position of electrodes during electrical stimulation has a very good effect on enhancing brain stimulation accuracy. At present, an increasing number of laboratories have begun to optimize tACS. However, there has been no study summarizing the optimization methods of tACS. Determining whether different optimization methods are effective and the optimization approach could provide information that could guide future tACS research. …

transcranial alternating current stimulationComputer scienceNeurosciences. Biological psychiatry. Neuropsychiatrychemical and pharmacologic phenomenaHuman NeuroscienceReviewhigh definition electrical stimulationintersectional short pulse methodBehavioral NeurosciencePsychiatry and Mental healthstomatognathic diseasesNeuropsychology and Physiological PsychologyNeurologystomatognathic systemBrain stimulationphase-shifted tACSElectronic engineeringOptimization methodstemporally interfering methodBiological Psychiatryamplitude modulated tACSRC321-571Transcranial alternating current stimulationFrontiers in Human Neuroscience
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Double copies of blaKPC-3::Tn4401a on an IncX3 plasmid in Klebsiella pneumoniae successful clone ST512 from Italy

2015

ABSTRACT A carbapenem-resistant sequence type 512 (ST512) Klebsiella pneumoniae carbapenemase 3 (KPC-3)-producing K. pneumoniae strain showing a novel variant plasmid content was isolated in Palermo, Italy, in 2014. ST512 is a worldwide successful clone associated with the spread of bla KPC genes located on the IncFIIk pKpQIL plasmid. In our ST512 strain, the bla KPC-3 gene was unusually located on an IncX3 plasmid, whose complete sequence was determined. Two copies of bla KPC-3 ::Tn 4401a caused by intramolecular transposition events were detected in the plasmid.

transposonsequence analysispolymerase chain reactionDrug ResistanceGene DosageSettore MED/42 - Igiene Generale E Applicatabacterial proteinbeta-Lactamaseopen reading framecarbapenemasePlasmidminocyclineplasmid DNAmeropenemPharmacology (medical)geneticscolistincefpodoximeceftazidime610 Medicine & healthCarbapenemBacterialpolymyxin Btimentingene expression regulationbacteriumKlebsiella pneumoniae carbapenemase 3 producing Klebsiella pneumoniae3. Good healthantiinfective agentmicrobial sensitivity testKlebsiella pneumoniaeItalypriority journaltigecyclineMultipleclone (Java method)cefotaxime030106 microbiologyKlebsiella pneumoniae carbapenemase 3tobramycinMicrobial Sensitivity Testsgentamicinpiperacillin plus tazobactamchemistryGene dosageArticleMicrobiology03 medical and health sciencesComplete sequenceClone CellOpen Reading FramesertapenemBacterial Proteinsmultidrug resistanceextensively drug resistant bacteriumAnti-Bacterial AgentcefepimePharmacologylevofloxacinmicrobiologycefoxitinbiochemical phenomena metabolism and nutritionbacterial infections and mycosesVirologyAnti-Bacterial Agents; Bacterial Proteins; Carbapenems; Clone Cells; Drug Resistance Multiple Bacterial; Gene Dosage; Italy; Klebsiella Infections; Klebsiella pneumoniae; Microbial Sensitivity Tests; Open Reading Frames; Plasmids; beta-Lactamases; DNA Transposable Elements; Gene Expression Regulation Bacterial; Pharmacology (medical); Pharmacology; Infectious Diseasesantibiotic sensitivityClone CellsKlebsiella InfectionsceftriaxoneCarbapenemsbacterial genetics0301 basic medicinemolecular cloningSettore MED/07 - Microbiologia E Microbiologia ClinicaKlebsiella pneumoniaeTransposition (music)Drug Resistance Multiple Bacterialpolycyclic compoundsgenetic screeningcell clonecarbapenem derivativeKlebsiella infectionunclassified drugAnti-Bacterial AgentsInfectious Diseasesbacterial genePlasmidsenzymologydoripenemBiologyminimum inhibitory concentrationbeta-Lactamasesbeta lactamaseMechanisms of ResistanceciprofloxacinAmikacin; aztreonam; carbapenemase; cefepime; cefotaxime; cefoxitin; cefpodoxime; ceftazidime; ceftriaxone; ciprofloxacin; colistin; cotrimoxazole; doripenem; doxycycline; ertapenem; gentamicin; imipenem; Klebsiella pneumoniae carbapenemase 3; levofloxacin; meropenem; minocycline; piperacillin plus tazobactam; plasmid DNA; polymyxin B; tigecycline; timentin; tobramycin; unclassified drug; antiinfective agent; bacterial protein; beta lactamase; carbapenem derivative; transposon antibiotic sensitivity; Article; bacterial gene; bacterial genetics; bacterial strain; bacterium; bacterium detection; bacterium isolation; Escherichia coli; extensively drug resistant bacterium; gene dosage; genetic screening; Italy; Klebsiella pneumoniae; Klebsiella pneumoniae carbapenemase 3 producing Klebsiella pneumoniae; minimum inhibitory concentration; molecular cloning; nonhuman; polymerase chain reaction; priority journal; sequence analysis; cell clone; chemistry; drug effects; enzymology; gene expression regulation; genetics; isolation and purification; Klebsiella infection; Klebsiella pneumoniae; metabolism; microbial sensitivity test; microbiology; multidrug resistance; open reading frame; plasmid; transposon Anti-Bacterial Agents; Bacterial Proteins; beta-Lactamases; Carbapenems; Clone Cells; DNA Transposable Elements; Drug Resistance Multiple Bacterial; Gene Dosage; Gene Expression Regulation Bacterial; Italy; Klebsiella Infections; Klebsiella pneumoniae; Microbial Sensitivity Tests; Open Reading Frames; Plasmidsplasmidbacterium isolationEscherichia coliGeneAmikacinbacterium detectionnonhumandoxycyclineisolation and purificationGene Expression Regulation Bacterialbiology.organism_classificationbacterial straincotrimoxazoleOpen reading frameDNA Transposable Elementdrug effectsDNA Transposable Elementsmetabolismaztreonamimipenem
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Diagnosis and prognosis of cardiovascular diseases by means of texture analysis in magnetic resonance imaging

2017

Cardiovascular diseases constitute the leading global cause of morbidity and mortality. Magnetic resonance imaging (MRI) has become the gold standard technique for the assessment of patients with myocardial infarction. However, limitations still exist thus new alternatives are open to investigation. Texture analysis is a technique that aims to quantify the texture of the images that are not always perceptible by the human eye. It has been successfully applied in medical imaging but applications to cardiac MRI (CMR) are still scarce. Therefore, the purpose of this thesis was to apply texture analysis in conventional CMR images for the assessment of patients with myocardial infarction, as an …

tratamiento digital de imágenesdiagnóstico por imagenmyocardial infarctionmachine learningcardiovascular systemcardiovascular diseasesanálisis de datoscardiac magnetic resonancetexture analysisresonancia magnética
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Effect of RNS60 in amyotrophic lateral sclerosis: a phase II multicentre, randomized, double-blind, placebo-controlled trial

2022

Background and purpose: Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease with limited treatment options. RNS60 is an immunomodulatory and neuroprotective investigational product that has shown efficacy in animal models of ALS and other neurodegenerative diseases. Its administration has been safe and well tolerated in ALS subjects in previous early phase trials. Methods: This was a phase II, multicentre, randomized, double-blind, placebo-controlled, parallel-group trial. Participants diagnosed with definite, probable or probable laboratory-supported ALS were assigned to receive RNS60 or placebo administered for 24 weeks intravenously (375 ml) once a week and via nebul…

treatmentAmyotrophic Lateral Sclerosisplacebo-controlledNeurodegenerative DiseasesALS; clinical trial; placebo-controlled; randomized; treatmentclinical trialTreatment OutcomeNeurologyDouble-Blind MethodrandomizedQuality of LifeHumansSettore MED/26 - NeurologiaNeurology (clinical)ALSBiomarkers
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