Search results for "Disulfide"
showing 10 items of 171 documents
CCDC 263593: Experimental Crystal Structure Determination
2005
Related Article: A.Laromaine, F.Teixidor, R.Kivekas, R.Sillanpaa, R.Benakki, B.Gruner, C.Vinas|2005|Dalton Trans.||1785|doi:10.1039/b502328a
CCDC 980426: Experimental Crystal Structure Determination
2014
Related Article: Almudena Gallego, Oscar Castillo, Carlos J. Gómez-García, Félix Zamora, Salome Delgado|2014|Eur.J.Inorg.Chem.||3879|doi:10.1002/ejic.201400085
CCDC 660922: Experimental Crystal Structure Determination
2010
Related Article: M.Ohba, K.Yoneda, G.Agusti, M.C.Munoz, A.B.Gaspar, J.A.Real, M.Yamasaki, H.Ando, Y.Nakao, S.Sakaki, S.Kitagawa|2009|Angew.Chem.,Int.Ed.|48|4767|doi:10.1002/anie.200806039
CCDC 775642: Experimental Crystal Structure Determination
2011
Related Article: C.J.Adams, J.A.Real, R.E.Waddington|2010|CrystEngComm|12|3547|doi:10.1039/c0ce00149j
CCDC 263594: Experimental Crystal Structure Determination
2005
Related Article: A.Laromaine, F.Teixidor, R.Kivekas, R.Sillanpaa, R.Benakki, B.Gruner, C.Vinas|2005|Dalton Trans.||1785|doi:10.1039/b502328a
Programmed Formation of HCN Oligomers through Organosulfur Catalysis
2021
An efficient, inexpensive, and reliable synthesis of diaminomaleonitrile (DAMN, 1) is described starting from readily available acetone cyanohydrin as the source of hydrogen cyanide (HCN). Diaminomaleonitrile (DAMN) is known to be an important intermediate in heterocyclic and medicinal chemistry as well as being a possible precursor for the origin of life's hypothesis within prebiotic chemistry. The mechanism of its formation through organosulfur catalysis has been investigated by electrospray ionization mass spectrometry (ESI-MS) using two newly synthesized cationic "marker" molecules as a tool that allows for sensitive detection. As a result, the proposed mechanism of a thiocyanate-mediat…
Detection and localisation of disulphide bonds in a synthetic peptide reproducing the sequence 1-30 of Par j 1.0101 by electrospray ionisation mass s…
2001
The structural characterisation of a synthetic peptide reproducing the sequence 1–30 of Par j 1.0101, a major allergenic protein present in the pollen of Parietaria judaica, by combined use of chemical and enzymatic cleavage, reversed-phase high-performance liquid chromatography (RP-HPLC) and electrospray ionisation mass spectrometry (ESI-MS), is described. Direct ESI-MS of the synthetic peptide after reaction with methyl iodide showed that the product is a mixture of two peptides: one form in which two out of the four cysteine residues present in the sequence are oxidised and a minor amount of another form in which all the cysteines are fully reduced. It was ascertained, using the combined…
Characterization of heat-labile toxin-subunit B from Escherichia coli by liquid chromatography-electrospray ionization-mass spectrometry and matrix-a…
2012
The possibilities of characterizing the heat-labile enterotoxin (LT) of enterotoxigenic Escherichia coli (ETEC) by liquid chromatography electrospray mass spectrometry (LC/ESI-MS) and matrix-assisted laser desorption with time-of-flight mass spectrometry (MALDI-TOF-MS) were investigated. The B subunit from recombinant E. coli (expression in Pichia pastoris) can be detected by LC/ESI-MS expressed in P. pastoris and the charge envelope signals can be observed; LC/ESI-MS and MALDI-TOF-MS analysis allowed the acquisition of labile toxin subunit B (LTB) molecular weight and preliminary structural characterization of LTB toxin. MALDI-TOF analysis after reduction and alkylation of the protein evid…
Rethinking Cysteine Protective Groups:S-Alkylsulfonyl-l-Cysteines for Chemoselective Disulfide Formation
2016
The ability to reversibly cross-link proteins and peptides grants the amino acid cysteine its unique role in nature as well as in peptide chemistry. We report a novel class of S-alkylsulfonyl-l-cysteines and N-carboxy anhydrides (NCA) thereof for peptide synthesis. The S-alkylsulfonyl group is stable against amines and thus enables its use under Fmoc chemistry conditions and the controlled polymerization of the corresponding NCAs yielding well-defined homo- as well as block co-polymers. Yet, thiols react immediately with the S-alkylsulfonyl group forming asymmetric disulfides. Therefore, we introduce the first reactive cysteine derivative for efficient and chemoselective disulfide formation…
Coupling of the heme and an internal disulfide bond in human neuroglobin
2004
Neuroglobin displays a hexacoordination His-Fe-His in the absence of external ligands such as oxygen. The observed oxygen affinity therefore depends on the binding rates of both oxygen and the competing distal histidine. Furthermore, the binding properties depend on the presence of an internal disulfide bond. In the case of human neuroglobin, cysteines at positions CD7 and D5 are sufficiently close to form an internal disulfide bond. For cytoglobin, the cysteine residues at positions A7 and GH4 may also form a disulfide bond. Mass spectrometry, ligand binding, and thiol accessibility studies were used to study the role influence of these disulfide bonds. Mutation of specific cysteines, or r…