Search results for "Dose"
showing 10 items of 2247 documents
The gas chromatography/mass spectrometry can be used for dose estimation in irradiated pork
2009
Abstract Food safety can be improved using ionizing radiation to reduce food spoilage and to extend its shelf life. The gas chromatography/mass spectrometry (GC/MS) has been validated by the European Community as a powerful method to identify irradiated food containing fat. The preliminary goals of our research were: (i) to set up this method, based on the detection of radiation induced 2-dodecylcyclobutanones (2-DCB) in pork muscle samples and (ii) to check the microbiological efficacy of the treatment. The main objective was to render the GC/MS a quantitative technique for dose estimation, through the measurement of the 2-DCB concentration in the irradiated sample. Our results show that t…
Late Breaking Abstract - Dose-ranging study of mepolizumab in eosinophilic COPD
2018
Background: Patients with eosinophilic COPD & frequent exacerbations may benefit from mepolizumab (MEP) treatment. Objective: Assess efficacy & safety of 2 MEP doses vs placebo (PBO) in COPD with blood eosinophils ≥150 cells/µL [screening] or ≥300 cells/µL [prior yr], history of ≥2 moderate/≥1 severe exacerbations & ICS+≥2 bronchodilator maintenance therapies (ICS+MT). Methods: Phase III, randomised, PBO-controlled, double-blind, parallel-group trial (METREO); patients received SC MEP 100mg, MEP 300mg or PBO plus ICS+MT, every 4 wks for 52 wks. Primary endpoint: rate/yr of moderate (systemic corticosteroids/antibiotics)/severe(hospitalisation or death) exacerbations. Secondary endpoints inc…
Fluence rate or cumulative dose? : Vulnerability of larval northern pike (Esox lucius) to ultraviolet radiation
2007
Newly hatched larvae of northern pike were exposed in the laboratory to four fluence rates of ultraviolet radiation (UVR; 290–400 nm) over three different time periods, resulting in total doses ranging from 3.0 ± 0.2 to 63.0 ± 4.4 kJ·m−2. Mortality and behavior of the larvae were followed for 8–12 days, and growth measured at the end of the experiment. Also, the principle of reciprocity—that the UVR-induced mortality depends on the cumulative dose, independent of fluence rate—was tested. Fluence rates higher than 1480 ± 150 mW·m−2 caused mortality and growth retardation. The highest fluence rate (3040 ± 210 mW·m−2) caused 100% mortality in 5 days. All fluence rates caused behavioral disorde…
Retinoids as a Perspective in Treatment of Alzheimer’s Disease
2010
<i>Background:</i> In the past, we demonstrated that the disintegrin metalloproteinase ADAM10 has α-secretase activity in vitro and in cultured cells. We also found out that moderate overexpression of this proteinase inhibits Aβ peptide production and prevents the formation of amyloid plaques in an Alzheimer’s disease (AD) mouse model. Moreover, it corrects early hippocampal defects like LTP impairment and increases cortical synaptogenesis. <i>Objective:</i> Upregulation of ADAM10 might be an alternative approach concerning AD therapy. Our current research therefore focuses on substances and/or pathways which regulate ADAM10 gene expression. <i>Methods:</i&g…
The role of mitochondria in sterigmatocystin-induced apoptosis on SH-SY5Y cells
2020
Mitochondria are cellular organelles involved in many crucial functions, such as generation of energy (ATP) and initiation of apoptosis. The aim of the present study was to evaluate the role of mitochondria in the toxicity induced by sterigmatocystin (STE), a mycotoxin produced by fungi of the genus Aspergillus, on SH-SY5Y cells. Our results showed that STE exposure decreased cell viability in a time- and concentration-dependent manner by MTT assay and caused mitochondrial dysfunction, as highlighted by the increase of STE cytotoxicity in cells forced to rely on mitochondrial oxidative phosphorylation. Furthermore, intracellular ATP depletion and increased mitochondrial reactive oxygen spec…
Relaxation induced by N-terminal fragments of chromogranin A in mouse gastric preparations.
2007
Abstract A definitive role for chromogranin A (CGA)-derived fragments in the control of the gastrointestinal smooth muscle contractility has not been yet established. The purpose of the present study was to evaluate, in vitro , the effects of the recombinant vasostatin 1–78 (VS-1), CGA 7–57 and CGA 47–66 on the mouse gastric mechanical activity, recording the changes of intraluminal pressure. VS-1, CGA 7–57 and CGA 47–66 produced concentration-dependent relaxations. Mouse anti-vasostatin-1 monoclonal antibody 5A8, recognising the region 53–57, abolished the relaxation induced by VS-1, indicating the specificity of the effect. The relaxation was significantly reduced by tetrodotoxin (TTX), b…
Humic acid reduces gonadotropin activity and hormonal sensitivity of frog oocytes
2005
The specific stimulatory effect of sturgeon Acipenser güldenstädti Br. gonadotropic hormone (GTH) on frog Rana temporaria L. oocyte maturation in vitro was investigated in relation to humic acid (HA) concentrations from 12.5 to 50 mg/l. HA was observed to bind to both the follicular membrane of the oocytes and the GTH molecule, reducing the oocytes' hormone sensitivity and maturation ability. It was also shown that HA inactivated GTH, lowering its specific ability to stimulate oocyte maturation.
UNEDF-parametrisaatioiden ennustamien sidosenergioiden tilastolliset virheet
2015
Tämän pro gradu -tutkielman tavoitteena oli tutkia UNEDF0, UNEDF1 ja UNEDF2-parametrisaatioiden ennustamia sidosenergioita ja näiden tilastollisia virheitä eri ytimille HFBTHO-tietokone-ohjelmaa käyttäen. Tilastollisena virheenä käytin keskihajontaa. Tarkasteltavana olivat parillis-parilliset Gd-ytimet (A=130−180), parillis-parilliset Dy-ytimet (A=140−182) sekä UNEDF1- ja UNEDF2-parametrisaatioissa parametrien sovitukseen käytetyt ytimien U-226, U-238, Pu-240 ja Cm-242 fissioisomeerit. Vertasin laskennallisia sidosenergioita ja näiden virheitä niin toisiinsa kuin kokeellisesti määritettyihin arvoihin. Lisäksi tutkin, mitkä käytettyjen mallien parametreista ovat eniten vastuussa tilastollise…
Biological responses of Rhynchophorous ferrugineus to Steirnenema carpocapase: an example of a model system
2011
Rhynchophorus ferrugineus (Coleoptera: Curculionidae) known as the Red Palm Weevil (RPW) is becoming more and more of a problem in Italy, and especially in Sicily, where it is well adapted. The infestations are mainly in the urban areas, and for that reason, chemical control is not advisable. Data from literature show that entomopathogenic nematodes (EPN) control RPW quite successfully in the field. However, results coming from the laboratories are often in contrast with each other and no data are available on precise doses. In this context we studied the Median Lethal Dose (DL50) and the Median Lethal Time (TL50) of young and older larvae and also of adults. The doses for the adults were t…
Responses of Rhynchophorus ferrugineus adults to selected synthetic palm esters: electroantennographic studies and trap catches in an urban environme…
2011
The Red Palm Weevil (RPW), Rhynchophorus ferrugineus Olivier, accidentally introduced in the European countries facing the Mediterranean basin, is becoming the most serious pest for ornamental palms in the urban environment. In our work we tested electroantennogram (EAG) and behavioural responses of RPW adults to five selected synthetic palm esters: ethyl propionate, ethyl butyrate, ethyl isobutyrate, ethyl lactate and ethyl acetate. EAG bioassays showed higher sensitivity of female antennae compared to male antennae for test compounds. Differences were also recorded in the EAG responses using different esters and doses. Ethyl propionate was the synthetic ester that elicited the strongest r…