Search results for "Down"
showing 10 items of 1658 documents
Osteoprotegerin expression in liver is induced by IL-13 through TGF-β
2020
1.AbstractBackgroundsOsteoprotegerin (OPG) is a profibrotic mediator produced by myofibroblasts under influence of transforming growth factor β (TGFβ). Its expression in experimental models of liver fibrosis correlates well with disease severity and treatment responses. The regulation of OPG in liver tissue is largely unknown and we therefore set out to elucidate which growth factors/interleukins associated with fibrosis induce OPG and through which pathways.MethodsPrecision-cut liver slices of wild type and STAT6-deficient mice and 3T3 fibroblasts were used to investigate the effects of TGFβ, interleukin (IL) 13 (IL13), IL1β, and platelet-derived growth factor BB (PDGF-BB) on expression of…
Muscleblind, BSF and TBPH are mislocalized in the muscle sarcomere of a Drosophila myotonic dystrophy model
2012
SummaryMyotonic dystrophy type 1 (DM1) is a genetic disease caused by the pathological expansion of a CTG trinucleotide repeat in the 3' UTR of the DMPK gene. In the DMPK transcripts, the CUG expansions sequester RNA-binding proteins into nuclear foci, including transcription factors and alternative splicing regulators such as MBNL1. MBNL1 sequestration has been associated with key features of DM1. However, the basis behind a number of molecular and histological alterations in DM1 remain unclear. To help identify new pathogenic components of the disease, we carried out a genetic screen using a Drosophila model of DM1 that expresses 480 interrupted CTG repeats, i(CTG)480, and a collection of…
Type V collagen counteracts osteo-differentiation of human mesenchymal stem cells
2014
In search of novel gene signatures for osteo-differentiation of mesenchymal stem cells (MSCs), we submitted cDNA preparations from undifferentiated and differentiating MSCs to differential display- and semiquantitative-PCR and found down-regulation of COL5A1 in osteo-induced cultures at days 21 and 28, when the mineralized matrix accumulates. We also cultured osteo-differentiating MSCs onto type V collagen substrates and found a decrease in the accumulation of extracellular calcium compared to those grown in uncoated flasks. To our knowledge, this is first evidence that type V collagen might represent a stromal component that impairs osteogenesis.
The Epidemiology of Hip and Knee Primary and Revision Arthroplasties during the COVID-19 Pandemic
2021
Background: The purpose of this study was to provide a comprehensive assessment of the impact of the COVID-19 pandemic on the epidemiology of primary and revision arthroplasties of the hip and knee joint. Methods: This study compared the data on knee and hip arthroplasty procedures from 2 hospitals (primary and revision) conducted in two periods: the period of the COVID-19 pandemic in Poland (from 4 March 2020 to 15 October 2020) and the corresponding period prior to the pandemic (from 4 March 2019 to 15 October 2019). We compared the epidemiological data, demographic data, and hospital stay duration data from these two periods. Results: Our analysis demonstrated that the total number of hi…
Realne i cudowne elementy w narracji o dziejach pierwszej krucjaty (na przykładzie Gesta Francorum)
2018
Pierwsza krucjata była z pewnością jednym z przełomowych wydarzeń europejskiego średniowiecza, a opowieści o niej ukształtowały kolejne pokolenia mieszkańców starego kontynentu. Jedną z takich opowieści są Gesta Francorum, w których znajdujemy ciągłą i zwartą narrację, obejmującą zarówno wydarzenia, które dziś ocenilibyśmy jako realistyczne, jak i wątki cudowne. Czy jednak historyk może odciąć pierwsze od drugich metodologicznym nożem, jednocześnie zachowując pewność, że swej analizie poddał całość badanego tekstu? Aby uniknąć podobnego działania, spróbowaliśmy przystawić do tej konkretnej opowieści o krucjacie metodologię ritual studies, która pozwoliła na badanie tekstu jako całości, wyko…
The Major Heat Shock Proteins, Hsp70 and Hsp90, in 2-Methoxyestradiol-Mediated Osteosarcoma Cell Death Model
2020
2-Methoxyestradiol is one of the natural 17&beta
Proyecto de un Estudio Piloto sobre las lesiones más frecuentes del pie en niños con Síndrome de Down
2022
El Síndrome de Down (SD) es la cromosomopatía más común y la causa genética más frecuente de discapacidad intelectual y malformaciones genéticas en el mundo. Los avances en los métodos de diagnóstico y tratamiento de este síndrome están aumentando la esperanza de vida de estas personas, sin embargo, es importante comenzar una valoración podológica para asegurar el desarrollo y crecimiento óptimo de estos niños. Este estudio ha sido diseñado para valorar el tipo de pie que tienen los niños de 0 a 16 años basándose en la evidencia y tratando de facilitar su aplicación en la práctica. Material y métodos: La finalidad es realizar pruebas clínicas y exploratorias para cuantificar la posición que…
Recenti tendenze in tema di responsabilità degli intermediari di Internet nella tradizione giuridica occidentale
2019
Il saggio prende in esame il dibattito circa la necessità di modificare gli attuali regimi di irresponsabilità degli intermediari della rete, dibattito in corso nelle esperienze statunitense ed unitaria europea. Con riferimento all’esperienza statunitense, non è chiaro, infatti, se il ricorso sempre più frequente alla procedura di notice and take down sia sintomo del successo del sistema di notice based liability, congegnato dal legislatore federale nel 1998 o sia, al contrario, espressione del suo fallimento. In tale ottica, al fine di verificare la possibilità di transitare da un sistema di notice and take down ad un sistema di notice and stay down, il saggio prende in esame le recenti in…
High-Fat Diet Induces Pre-Diabetes and Distinct Sex-Specific Metabolic Alterations in Negr1-Deficient Mice
2021
In the large GWAS studies, NEGR1 gene has been one of the most significant gene loci for body mass phenotype. The purpose of the current study was to clarify the role of NEGR1 in the maintenance of systemic metabolism, including glucose homeostasis, by using both male and female Negr1−/− mice receiving a standard or high fat diet (HFD). We found that 6 weeks of HFD leads to higher levels of blood glucose in Negr1−/− mice. In the glucose tolerance test, HFD induced phenotype difference only in male mice
Dynamic shakedown design of structures under repeated seismic loads
2013
The paper is devoted to the formulation of an optimal design (minimum volume) problem of elastic perfectly plastic structures subjected to suitable combinations of static (fixed) and dynamic (seismic) loads. The structure is constrained to simultaneously respect two different safety criteria; actually, it must exhibit an elastic shakedown behaviour for the combination of loads characterizing the serviceability conditions and it must prevent the instantaneous collapse for the highest expected load condition (combination of loads characterized by the presence of fixed loads and maximum expected intensity of seismic action). The shakedown limit behaviour for the optimal structure will be impos…