Search results for "Down"
showing 10 items of 1658 documents
Borrelia burgdorferi ārējās virsmas proteīnu BB0689 un BBA73 funkcionālie pētījumi
2015
Maģistra darbs veidots ar mērķi noskaidrot B. burgdorferi ārējās virsmas proteīnu BB0689 un BBA73 potenciālo lomu patogēna un saimniekorganisma mijiedarbībā, kas varētu paplašināt mūsu zināšanas par Laimboreliozes patoģenēzi. Borēlijas proteīnu un zīdītāju vai artropodu proteīnu iespējamā mijiedarbība ir noteikta, izmantojot Ni-NTA afinitātes hromatogrāfiju, proteīnu kompleksu imūnprecipitāciju (Co-Ip) un attālināto Western blotu. Konstatēto mijiedarbības partneru identifikācijai ir izmantota MALDI-TOF masas spektrometrija. Pētījuma rezultātā ir konstatēta BB0689 iespējamā saistība ar vairākiem zīdītāju proteīniem, savukārt BBA73 gadījumā ir novērota tā mijiedarbība ar Ixodes ērču proteīnie…
Shakedown Analysis by Elastic Simulation
2000
Shakedown analysis of elastic plastic structures is widely credited as a valuable analytical/numerical tool for design purposes. For complex structures and loading conditions, e. g. for fast breeder nuclear reactor plants, full inelastic analysis is rarely performed, practically never within the early stages of the design advancement and the inherent decision process. The essential information therein needed can in fact be obtained, at moderate computational costs, by application of the shakedown methods and rules, at least within some limits related to the present developments of shakedown theory and its applicability to practical engineering problems, see e. g. Ponter et al. (1990), Carte…
Microwave and optical data fusion for global mapping of soil moisture at high resolution
2018
After more than 8 years in orbit the Soil Moisture and Ocean Salinity (SMOS) satellite is still in good health and several algorithms for improving its spatial resolution have been proposed and validated in a variety of catchments. However, none of them has yet been applied at the global scale. In this article we present: i) a review of the latest SMOS-BEC downscaling algorithm, which allows for its global application using an adaptive moving window and ii) a thorough validation of the resulting maps over two in-situ networks: REMEDHUS in Spain and OzNet in Australia. The proposed algorithm combines SMOS brightness temperatures (at ~40 km spatial resolution), and MODIS-derived Land Surface …
Laser-induced plasma spectroscopy in near vacuum ultraviolet using ordinary spectrograph and ICCD
2002
An experimental setup to measure laser-induced plasma emission spectra with an ordinary Czerny-Turner spectrograph and intensified charge-coupled device in the near vacuum ultraviolet down to 130 nm is described. Spectra of bromine, chlorine and iodine were recorded to demonstrate the performance of the setup.
A Wnt-specific astacin proteinase controls head formation inHydra
2020
AbstractTheHydrahead organizer acts as a signaling center that initiates and maintains the primary body axis in steady state polyps and during budding or regeneration. Wnt/beta-Catenin signaling functions as a primary cue controlling this process, but how Wnt ligand activity is locally restricted at the protein level is poorly understood.Here we report the identification of an astacin family proteinase as a Wnt processing factor.Hydraastacin-7 (HAS-7) is expressed from gland cells as an apical-distal gradient in the body column, peaking close beneath the tentacle zone.HAS-7siRNA knockdown abrogates HyWnt3 proteolysis in the head tissue and induces a robust double axis phenotype, which is re…
Large eddy simulation model for wind-driven sea circulation in coastal areas
2013
Abstract. In the present paper a state-of-the-art large eddy simulation model (LES-COAST), suited for the analysis of water circulation and mixing in closed or semi-closed areas, is presented and applied to the study of the hydrodynamic characteristics of the Muggia bay, the industrial harbor of the city of Trieste, Italy. The model solves the non-hydrostatic, unsteady Navier–Stokes equations, under the Boussinesq approximation for temperature and salinity buoyancy effects, using a novel, two-eddy viscosity Smagorinsky model for the closure of the subgrid-scale momentum fluxes. The model employs: a simple and effective technique to take into account wind-stress inhomogeneity related to the …
Organy w kościele Świętego Krzyża w Bytomiu-Miechowicach. Historia i koncepcja rekonstrukcji
2018
Th article describes the history of organs in the noble Church of the Holy Cross in BytomMiechowice. Th largest amount of information has been preserved about the instrument built in 1905 by the Schlag und Söhne company from Świdnica. Ths organ was dismantled in the 1960s due to heavy mining damage and was later replaced by a temporary instrument. However, work is currently underway on the renovation of the temple. A proposal to recreate the historical organ is among the plans and is included in the ongoing design work.
Obesity alters the gustatory perception of lipids in the mouse: plausible involvement of lingual CD36. : Obesity decreases the fat preference
2013
International audience; A relationship between orosensory detection of dietary lipids, regulation of fat intake, and body mass index was recently suggested. However, involved mechanisms are poorly understood. Moreover, whether obesity can directly modulate preference for fatty foods remains unknown. To address this question, exploration of the oral lipid sensing system was undertaken in diet-induced obese (DIO) mice. By using a combination of biochemical, physiological, and behavioral approaches, we found that i) the attraction for lipids is decreased in obese mice, ii) this behavioral change has an orosensory origin, iii) it is reversed in calorie-restricted DIO mice, revealing an inverse …
Treatment of T Cell-Dependent Experimental Colitis in SCID Mice by Local Administration of an Adenovirus Expressing IL-18 Antisense mRNA
2001
Abstract Recent studies have shown that IL-18, a pleiotropic cytokine that augments IFN-γ production, is produced by intestinal epithelial cells and lamina propria cells from patients with Crohn’s disease. In this study, we show that IL-18 is strongly expressed by intestinal epithelial cells in a murine model of Crohn’s disease induced by transfer of CD62L+CD4+ T cells into SCID mice. To specifically down-regulate IL-18 expression in this model, we constructed an E1/E3-deleted adenovirus expressing IL-18 antisense mRNA, denoted Ad-asIL-18, and demonstrated the capacity of such a vector to down-regulate IL-18 expression in colon-derived DLD-1 cells and RAW264.7 macrophages. Local administrat…
Cutting Edge: TGF-β Induces a Regulatory Phenotype in CD4+CD25− T Cells through Foxp3 Induction and Down-Regulation of Smad7
2004
Abstract CD4+CD25+ regulatory cells are a subpopulation of T lymphocytes of thymic origin. However, recent data suggest an alternative commitment of regulatory T cells in the periphery, although the precise mechanism is unknown. In the present work, we demonstrate that TGF-β is able to induce Foxp3 expression and subsequently a regulatory phenotype in CD4+CD25− peripheral murine T cells. Similarly, TGF-β induced Foxp3 in human CD4+CD25− T cells. Moreover, we show that the inhibitory Smad7 protein that is normally induced by TGF-β and limits TGF-β signaling, is strongly down-regulated by Foxp3 at the transcriptional level. Foxp3-mediated down-regulation of Smad7 subsequently rendered CD4+CD2…