Search results for "Dox"
showing 10 items of 1345 documents
Measurements of cytochrome f and P-700 in intact leaves of Sinapis alba grown under high-light and low-light conditions
1978
The oxidation and reduction of cytochrome f and P-700 is measured spectrophotometrically in leaves of low-light and high-light plants. After illumination with red light, an induction phenomenon for cytochrome f oxidation is observed which indicates a regulation of photosystem I activity through energy distribution between the pigment systems by the energy state of the membrane. After far-red excitation the reduction of cytochrome f in the dark is much slower in low-light leaves. This shows that cyclic electron transport is not improved in low-light plants under these conditions. P-700 is oxidized on excitation with far-red light. However, with high intensities of far-red light, P-700 is par…
Studies on the Content of P 700 and Cytochromes in Sinapis alba During Growth Under Two Different Light Intensities
1975
Sinapis alba was grown under two extremely different light intensities. We obtained two different modifications which hold several physiological and biochemical characteristics comparable to the relations in light- and shade plants. In the strong light the maximum rate of CO2-uptake increases within five days to a three-fold rate. At the same time the content of several redox systems of photosynthetic electron transport increases while the content of P 700 remains equivalent in both types of plants. In the high-light plants we find a ratio P 700: Cytochrome f of 1 but a ratio of 3 in the low-light plants. These ratios could be probably essential features of various sun- and rainforest speci…
Changes in the Content of Chlorophyll and Redox Components of the Thylakoid Membrane during Development and Senescence of Beech (Fagus sylvatica) Lea…
1995
Abstract Leaves from 145-year-old and 44-year-old beech trees were harvested during 1991-1993. Chlorophyll (Chl) and redox components of the thylakoid membrane, including P-700, cytochrome f (Cyt f) and D1 protein, were determined with the following results. Chl a + b, P-700 and Cyt f per unit of fresh weight (FW), dry weight (DW) and leaf area (LA) increase significantly during leaf development. This can be attributed to a massive membrane synthesis and new thylakoid formation in the cells. The Chl a/Chl b ratio decreases with the synthesis of Chl during the development of beech leaves and is reduced further with Chl breakdown in the stage of senescence. When expressed on the basis of Chl…
Measurements of manganese in thylakoids of Sinapis alba grown under high-light and low-light conditions.
1981
The manganese content of thylakoids and tissues was measured in leaves grown under high- and low-light conditions. Especially when grown in a nutrient medium enriched in manganese (20 μM), the thylakoids contained large amounts of manganese, which could be removed by EDTA washing without impairment of the Hill reaction. The unremovable content of manganese was almost the same in thylakoids from plants grown in nutrient media of normal (2 μM) and reduced (0.2 μM) manganese content. Up to this limit of manganese content, Hill activity did not seem to be impaired. 1.2 atoms Mn per 100 molecules chlorophyll were found in low-light thylakoids and 1.6 atoms Mn in high-light thylakoids. This is si…
Chemotherapy Sensitizes Colon Cancer Initiating Cells to Vγ9Vδ2 T Cell-Mediated Cytotoxicity
2013
Colon cancer comprises a small population of cancer initiating stem cells (CIC) that is responsible for tumor maintenance and resistance to anti-cancer therapies, possibly allowing for tumor recapitulation once treatment stops. Combinations of immune-based therapies with chemotherapy and other anti-tumor agents may be of significant clinical benefit in the treatment of colon cancer. However, cellular immune-based therapies have not been experimented yet in the population of colon CICs. Here, we demonstrate that treatment with low concentrations of commonly used chemotherapeutic agents, 5- fluorouracyl and doxorubicin, sensitize colon CICs to Vc9Vd2 T cell cytotoxicity. Vc9Vd2 T cell cytotox…
Adaptive control of a seven mode truncation of the Kolmogorov flow with drag
2009
Abstract We study a seven dimensional nonlinear dynamical system obtained by a truncation of the Navier–Stokes equations for a two dimensional incompressible fluid with the addition of a linear term modelling the drag friction. We show the bifurcation sequence leading from laminar steady states to chaotic solutions with increasing Reynolds number. Finally, we design an adaptive control which drives the state of the system to the equilibrium point representing the stationary solution.
High Reynolds number Navier-Stokes solutions and boundary layer separation induced by a rectilinear vortex
2013
Abstract We compute the solutions of Prandtl’s and Navier–Stokes equations for the two dimensional flow induced by a rectilinear vortex interacting with a boundary in the half plane. For this initial datum Prandtl’s equation develops, in a finite time, a separation singularity. We investigate the different stages of unsteady separation for Navier–Stokes solution at different Reynolds numbers Re = 103–105, and we show the presence of a large-scale interaction between the viscous boundary layer and the inviscid outer flow. We also see a subsequent stage, characterized by the presence of a small-scale interaction, which is visible only for moderate-high Re numbers Re = 104–105. We also investi…
Identificación de nuevas rutas de patogénesis y dianas terapéuticas en la enfermedad de Parkinson mediante el uso de modelos biomédicos
2022
La enfermedad de Parkinson (EP) es la segunda enfermedad neurodegenerativa más común, caracterizada por la pérdida progresiva de neuronas dopaminérgicas en la substantia nigra pars compacta. Esto conduce a una disminución de los niveles de dopamina en el núcleo estriado, causando los síntomas motores típicos de la enfermedad. Entre las principales causas de la neurodegeneración se encuentran el estrés oxidativo (EO) y la disfunción mitocondrial. Aunque la mayoría de los casos de EP son idiopáticos, se ha demostrado que entre el 5 y el 10% de ellos son formas familiares de la enfermedad causadas por mutaciones en determinados genes. Uno de ellos es el gen DJ-1, cuyas mutaciones están asociad…
Lovastatin protects human endothelial cells from the genotoxic and cytotoxic effects of the anticancer drugs doxorubicin and etoposide
2006
Background and purpose: 3-Hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase inhibitors (statins) are frequently used lipid-lowering drugs. Moreover, they exert pleiotropic effects on cellular stress responses and death. Here, we analysed whether lovastatin affects the sensitivity of primary human endothelial cells (HUVEC) to the anticancer drug doxorubicin. Experimental approach: We investigated whether pretreatment of HUVEC with low dose of lovastatin influences the cellular sensitivity to doxorubicin. To this end, cell viability, proliferation and apoptosis as well as DNA damage-triggered stress response were analysed. Key results: Lovastatin reduced the cytotoxic potency of doxorub…
Ras-Related GTPase RhoB Forces Alkylation-Induced Apoptotic Cell Death
2000
rhoB encoding a Ras-related GTPase is immediate-early inducible by genotoxic treatments. To address the question of the physiological role of RhoB in cellular defense, cells stably overexpressing wild-type RhoB protein were generated. Overexpression of RhoB renders cells hypersensitive to the killing effect of alkylating agents including antineoplastic drugs but not to UV-light and doxorubicin. As compared to control cells, RhoB overexpressing cells revealed an increase in the frequency of alkylation-induced apoptotic cell death. This indicates that RhoB is involved in modulating apoptotic signaling. Furthermore, overexpression of RhoB resulted in a prolonged transient block to DNA replicat…