Search results for "Droplets"

showing 4 items of 44 documents

Intramyocellular topography of lipid droplets and OXPAT in obesity and type II diabetes

2010

Nowadays in the westernized world, high caloric diet and sedentarism are driving populations to accumulate ectopic fat, especially in skeletal muscle. Such is associated with the development of insulin resistance (IR) related metabolic diseases, as type II diabetes (T2D) and obesity (OB). Like IR individuals, athletes also show high intramyocellular lipids (IMCL) contents, denoting qualitative rather than quantitative differences between insulin sensitive (IS) and IR individuals IMCL. PAT proteins and OXPAT specifically, are among the main regulators of lipid droplets (LDs) in skeletal muscle. We hypothesized that different IS profiles are distinguishable through LD and OXPAT localization a…

mitochondriadiabeteslipid dropletsmitokondriotlipid metabolismlihaksetskeletal musclelipiditaineenvaihdunta
researchProduct

Comparison of acute and chronic exercise effects in the lipid droplets topography skeletal muscle, following high and low-fat diet in mice

2015

In the modern world, diet patterns high on lipids, sedentary lifestyle and obesity contribute all in the development of metabolic syndrome, which in turn can lead to type 2 diabetes. All the excess fats, that the majority of the population consumes nowadays, are stored as ectopic fat, particularly in the skeletal muscle, a tissue beyond doubt insulin-sensitive. Physical activity has been proposed according to various studies to be the most drastic factor, which can induce changes in skeletal muscle lipid metabolism,as well as different diet models. We hypothesized that different exercise patterns and diet-changes can alter the spatial arrangement in lipid droplets (LDs) in different skeleta…

obesitymicerasvatlipid dropletsphysical activityhiiretruokavaliotinsuliiniresistenssiinsulin resistancefiber typinglipid metabolismlihavuusdietaineenvaihduntafyysinen aktiivisuus
researchProduct

RAB18 Loss Interferes With Lipid Droplet Catabolism and Provokes Autophagy Network Adaptations

2020

Autophagy is dependent on appropriate lipid supply for autophagosome formation. The regulation of lipid acquisition and the autophagy network response to lipid-limiting conditions are mostly elusive. Here, we show that the knockout of the RAB GTPase RAB18 interferes with lipid droplet catabolism, causing an impaired fatty acid release. The resulting reduced lipid-droplet-derived lipid availability influences autophagy and provokes adaptive modifications of the autophagy network. These adjustments include increased expression and phosphorylation of ATG2B as well as augmented formation of the ATG12-ATG5 conjugate. Moreover, ATG9A shows an enhanced phosphorylation at amino acid residues tyrosi…

rab3 GTP-Binding ProteinsImmunoblottingGTPaseReal-Time Polymerase Chain Reaction03 medical and health sciences0302 clinical medicineMicroscopy Electron TransmissionStructural BiologyLipid dropletAutophagyHumansPhosphorylationTyrosineMolecular Biology030304 developmental biology0303 health sciencesMicroscopy ConfocalChemistryCatabolismAutophagyAutophagosomesLipid DropletsImmunohistochemistryCell biologyrab GTP-Binding ProteinsPhosphorylationlipids (amino acids peptides and proteins)RabCRISPR-Cas Systems030217 neurology & neurosurgeryRAB18HeLa CellsJournal of Molecular Biology
researchProduct

Microstructural evaluation and recommendations for face masks in community use to reduce the transmission of respiratory infectious diseases

2022

Funding Information: A.K., H.Y. and R.J. also acknowledges the funding through Academy of Finland BESIMAL (Decision No. 334197) and Aalto University, Department of Communications and Networking. This work has also received funding in part from the EPSRC UK (grant number EP/R012091/1). A.K. would also like to thank Mr. Volkan Kaplan for the fruitful discussions in the early concept generation. Publisher Copyright: © 2022 The Author(s) Background and Objective: Recommendations for the use of face masks to prevent and protect against the aerosols (≤5µm) and respiratory droplet particles (≥5µm), which can carry and transmit respiratory infections including severe acute respiratory syndrome coro…

virtauslaskentaSARS-CoV-2MasksCOVID-19Respiratory Aerosols and DropletsHealth InformaticsComputational fluid dynamicsCommunicable DiseasesComputer Science ApplicationskoronaviruksettaudinaiheuttajatAirborne pathogenX-ray microtomographykasvosuojaimetHumansaltistuminenFace masksFiltrationSoftware
researchProduct