Search results for "Droplets"
showing 4 items of 44 documents
HPMA-based block copolymers promote differential drug delivery kinetics for hydrophobic and amphiphilic molecules.
2015
Abstract We describe a method how polymeric nanoparticles stabilized with (2-hydroxypropyl)methacrylamide (HPMA)-based block copolymers are used as drug delivery systems for a fast release of hydrophobic and a controlled release of an amphiphilic molecule. The versatile method of the miniemulsion solvent-evaporation technique was used to prepare polystyrene (PS) as well as poly-d/l-lactide (PDLLA) nanoparticles. Covalently bound or physically adsorbed fluorescent dyes labeled the particles’ core and their block copolymer corona. Confocal laser scanning microscopy (CLSM) in combination with flow cytometry measurements were applied to demonstrate the burst release of a fluorescent hydrophobic…
Lipid Droplets: A New Player in Colorectal Cancer Stem Cells Unveiled by Spectroscopic Imaging
2015
Abstract The cancer stem cell (CSC) model is describing tumors as a hierarchical organized system and CSCs are suggested to be responsible for cancer recurrence after therapy. The identification of specific markers of CSCs is therefore of paramount importance. Here, we show that high levels of lipid droplets (LDs) are a distinctive mark of CSCs in colorectal (CR) cancer. This increased lipid content was clearly revealed by label-free Raman spectroscopy and it directly correlates with well-accepted CR-CSC markers as CD133 and Wnt pathway activity. By xenotransplantation experiments, we have finally demonstrated that CR-CSCs overexpressing LDs retain most tumorigenic potential. A relevant con…
Comparison of acute and chronic exercise effects in the lipid droplets topography skeletal muscle, following high and low-fat diet in mice
2015
In the modern world, diet patterns high on lipids, sedentary lifestyle and obesity contribute all in the development of metabolic syndrome, which in turn can lead to type 2 diabetes. All the excess fats, that the majority of the population consumes nowadays, are stored as ectopic fat, particularly in the skeletal muscle, a tissue beyond doubt insulin-sensitive. Physical activity has been proposed according to various studies to be the most drastic factor, which can induce changes in skeletal muscle lipid metabolism,as well as different diet models. We hypothesized that different exercise patterns and diet-changes can alter the spatial arrangement in lipid droplets (LDs) in different skeleta…
Enlarged PLIN5-uncoated lipid droplets in inner regions of skeletal muscle type II fibers associate with type 2 diabetes
2022
Skeletal muscle physiology remains of paramount importance in understanding insulin resistance. Due to its high lipid turnover rates, regulation of intramyocellular lipid droplets (LDs) is a key factor. Perilipin 5 (PLIN5) is one of the most critical agents in such regulation, being often referred as a protector against lipotoxicity and consequent skeletal muscle insulin resistance. We examined area fraction, size, subcellular localization and PLIN5 association of LDs in two fiber types of type 2 diabetic (T2D), obese (OB) and healthy (HC) individuals by means of fluorescence microscopy and image analysis. We found that T2D type II fibers have a significant sub-population of large and inter…