Search results for "Drying"

Optimised lyophilisation-based method for different biomolecule single-extractions from the same rat brain sample: Suitability for RNA and protein ex…

2019

Abstract Background Optimisation of tissue processing procedures in preclinical studies reduces the number of animals used and allows integrated multilevel study in the same sample. Multiple extraction of different biomolecules from the same sample has several limitations. New method Using brain samples from rats subjected to ischemic stroke, we combined lyophilisation of flash-frozen tissue, mechanical pulverisation and cryopreservation in a method to optimise tissue handling and preservation for independent RNA or protein single-extract methods, and subsequent RT-qPCR or Western blot analyses. Results Lyophilisation resulted in 70% tissue weight loss. RNA (OD260/280∼1.8) and protein yield…

Comparing different processing methods in apple slice drying. Part 2 solid-state Fast Field Cycling 1H-NMR relaxation properties, shrinkage and chang…

2019

The objective of this paper was to study the effects of different drying methods that are: microwave (MW), hot air (HA) and a combination of both (HY), on the 1H-NMR relaxation properties, shrinkage and volatile compounds of Golden Delicious apple slices. Fast field cycling NMR relaxometry reveals that the HA samples dried at different temperatures (65 and 75 °C) appear to contain less restrained water as compared to the sample obtained by MW heating at the same temperatures. In fact, the longitudinal relaxation rates (T1) of the water molecules in the HA dried slices resulted shorter than those measured for the MW dried samples, thereby revealing that in the MW slices, water molecules beha…

Agronomical and postharvest evaluation of the essential oils of Sicilian rosemary (Rosmarinus officinalis L.) biotypes

2019

Rosmarinus officinalis L. is an aromatic shrub commonly found in the Mediterranean area. It is used in cooking both to add aroma and as a preservative in processed foods. Essential oils of rosemary are used in medicine and in the cosmetics industry due to their considerable biological properties. The yield and quality of rosemary essential oils mainly depend upon the chemo type, environmental conditions and cultivation techniques. The aim of this study was to evaluate the qualitative and quantitative characteristics of essential oils of wild R. officinalis biotypes found in Sicily using different drying methods. Five types of drying methods were used for the tests: a) shade drying under a r…

Selection of potential probiotic lactobacilli from pig feces to be used as additives in pelleted feeding

2006

Thirty-five isolates from pig feces were identified as Lactobacillus reuteri (12 strains), Lactobacillus mucosae (7), Lactobacillus plantarum (6), Lactobacillus kitasatonis (3), Lactobacillus rossiae (2), Lactobacillus ultunensis (2), Lactobacillus crispatus (2), and Lactobacillus intestinalis (1) by partial sequence analysis of the 16S rRNA. All isolates were detected at 8-9 log CFU g(-1). Preliminarily, strains were selected based on resistance to heat treatments (ca. 70 degrees C for 10 s). The decrease in viability for some L. reuteri, L. mucosae, L. plantarum, L. kitasatonis, and L. rossiae strains was lower than 1 log cycle. Selected strains were further characterized for acid and bil…

Solution-Based Processing for Scaffold Fabrication in Tissue Engineering Applications: A Brief Review

2021

The fabrication of 3D scaffolds is under wide investigation in tissue engineering (TE) because of its incessant development of new advanced technologies and the improvement of traditional processes. Currently, scientific and clinical research focuses on scaffold characterization to restore the function of missing or damaged tissues. A key for suitable scaffold production is the guarantee of an interconnected porous structure that allows the cells to grow as in native tissue. The fabrication techniques should meet the appropriate requirements, including feasible reproducibility and time- and cost-effective assets. This is necessary for easy processability, which is associated with the large …

Effect of Sunred metabolic promoter and deficit irrigation on fruit quality of 'Valencia' oranges

2012

This study evaluated the effect of deficit irrigation and Sunred foliar metabolic promoter on fruit quality of adult 'Valencia' orange trees. Three irrigation strategies were imposed in spring 2011: Irrigation with volumes corresponding to 100% of crop evapotranspiration applied to entire root-zone (CI), partial root-zone drying (PRD) with 50% of CI water applied to one alternated side of the root-zone, and continuous deficit irrigation (DI) with 50% of CI water applied to both sides of the root-zone. Four trees in each irrigation treatment were sprayed 30 and 20 days before harvest with Sunred metabolic promoter containing phenylalanine, methionine, mono-saccharides and oxylipins from plan…

Water status, growth, yield and fruit quality of 'Valencia' orange trees under partial root-zone drying

2011

Simple techniques suitable for long-term storage of Plasmopara viticola

2006

Summary. Propagules of Plasmopara viticola or fragments of P. viticola infected host tissue were stored by three techniques, freezing at –25°C, dehydration with CaCl2, and freeze-drying. Viability tests were performed periodically until exhaustion of the testing sample. P. viticola samples that were frozen at -25°C remained viable for eight years, whereas the CaCl2 dehydrated samples remained viable for eight and a half years. Freeze-drying was the most successful long-term storage method, being able to preserve samples for almost 21 years.

Effect of drying condition on Leonforte peaches

2013

Leonforte peaches ripen in September and October and even as late as November. They are wrapped in paper bags to protect them from the wind and parasites and are harvested only when perfectly ripe. Protected inside the bags, they ripen late and take on a bright yellow colour with red streaks. Wonderfully scented, the peach boasts yellow, firm flesh that is sweet with a distinctive, slightly caramelized flavour. Aim of this paper was to set a drying process for fresh fruit transformation. In fact, the fruit harvest is limited in a very short time, so is important to find a conservation method. Drying process was carried out in a pilot plant setting different temperature and time condition. T…

Sviluppo di spugne bioerodibili multifunzionali per la promozione di processi riparativi mediante il rilascio controllato di agenti antibiotici e ant…

2023

La salute delle strutture molli e dure del cavo orale è essenziale non solo per preservare le funzioni ad esse direttamente associate, ma anche per promuovere la salute generale dell’organismo. Tra le problematiche di maggiore interesse clinico a carico della cavità orale ritroviamo: i) le complicazioni post-operatorie che possono verificarsi a seguito di comuni avulsioni dentali che, se non trattate tempestivamente, possono sfociare in gravi parodontiti; ii) i fenomeni di osteonecrosi a carico di mascella e mandibola associate a trattamenti farmacologici nonché alla radioterapia; iii) il carcinoma orale a cellule squamose (OSCC) che è tra i più aggressivi tumori del collo e della testa, ca…