Search results for "Dye"
showing 10 items of 577 documents
Toward an integrative and predictive sperm quality analysis in Bos taurus
2017
There is a need to develop more integrative sperm quality analysis methods, enabling researchers to evaluate different parameters simultaneously cell by cell. In this work, we present a new multi-parametric fluorescent test able to discriminate different sperm subpopulations based on their labeling pattern and motility characteristics. Cryopreserved semen samples from 20 Holstein bulls were used in the study. Analyses of sperm motility using computer-assisted sperm analysis (CASA-mot), membrane integrity by acridine orange-propidium iodide combination and multi-parametric by the ISAS®3Fun kit, were performed. The new method allows a clear discrimination of sperm subpopulations based on memb…
A nitrergic projection from the superior olivary complex to the inferior colliculus of the rat
2003
The present study was conducted to test whether the ascending auditory projection from the superior olivary complex (SOC) of the brainstem to the inferior colliculus (IC) may use nitric oxide (NO) as a neuroactive compound. We identified olivo-collicular projection neurons in subnuclei of the SOC by retrograde neuronal tracing with Fluoro-Gold (FG) injected into the central nucleus of the IC. Sections containing retrograde labelled neurons were subjected to immunohistochemical incubation in an antiserum directed against the enzyme responsible for NO production in nerve cells, neuronal NO synthase (nNOS). The analysis showed that FG-containing neurons as well as nNOS-immunoreactive neurons w…
An approach to predictive testing of contact sensitizers in vitro by monitoring their influence on endocytotic mechanisms.
1996
Endocytotic activation of epidermal Langerhans cells (LC) by immunogenic haptens is an early event during development of allergic contact dermatitis. In this work a fast and objective flow-cytometric assay for predictive in vitro testing of contact sensitizers by monitoring their influence on endocytotic mechanisms in murine LC was developed. Epidermal cell suspensions were labelled with a monoclonal antibody directed to MHC class II molecules and pH-sensitive fluorochrome-coupled second-step reagents. For untreated LC a significant quenching of fluorescence intensity by internalization of the MHC-antibody complexes into acidic compartments was noticed. Similar results were obtained in the …
Diagnosing Helicobacter pylori in vivo by confocal laser endoscopy.
2005
Background & Aims: Confocal laser endomicroscopy enables subsurface microscopic imaging of living tissue during ongoing endoscopy. This case report describes the in vivo detection of Helicobacter pylori by endomicroscopy.Methods: Endomicroscopy (Pentax, Tokyo, EC-3870CIFK) was performed by using two different contrast stains: Topical Acriflavine in addition to intravenously applied fluorescein netted the surface and allowed identification of focal accumulation of Helicobacter pylori at the surface and in deeper layer of the gastric epithelium. Biopsies were performed at the antrum and corpus for urease testing and histology. In addition, biopsies were cultured for Helicobacter pylori. Cultu…
Principal neurons projecting to the pineal gland in close association with small intensely fluorescent cells in the superior cervical ganglion of rats
1988
The localization in the superior cervical ganglia (SCG) of small, intensely fluorescent (SIF) cells and of principal nerve (PN) cells innervating the pineal gland was examined in adult male Sprague-Dawley rats. PN cells were demonstrated by means of the retrograde neuron-tracing method using the fluorescent tracer Fluoro-Gold (FG) injected into the pineal gland. SIF cells were visualized by the formaldehyde-induced fluorescence method. Twenty-nine percent of the FG-labeled PN cells were found closely associated with SIF cells. In the rostral half of the ganglion, 43% of the SIF cells were situated in juxtaposition to one or several labeled neurons. The possible influence of SIF cells on the…
Characterization of Pigments from <i>Malus domestica</i> Leaves for Wool Dyeing
2021
The study will present results of chemical analysis of mass spectrometry, evaluating the content of pigments in M. domestica leaves dyeing solution and in dyed woollen yarn nowadays. Yarn was dyed with fresh leaves of M. domestica tree. Before dyeing the yarn was treated with potassium aluminium sulphate mordant. Dyestuffs from the yarn were extracted in compliance with modified hydrochloric method of hydrolysis. Extracts were analysed by ultra high-performance liquid chromatography coupled with a diode array detector and mass spectrometry (UHPLC-DAD-MS) for identification of the pigments. Phytochemical screening of dyeing solution of the M. domestica leaves showed presence of dihydrochalco…
Effect of silane coupling treatment on the adhesion between polyamide and epoxy based composites reinforced with carbon fibers
2020
The increasing efforts aimed to design structures with reduced weight and better mechanical performances has led in recent years to a growing use of fiber reinforced polymer materials in several fields such as marine. However, these materials can be composed of chemically very different elements and, hence, may be difficult to joint. This research aims to improve the adhesion between a thermoplastic matrix of polyamide reinforced with short carbon fibers (PA12-CR) and a carbon fiber reinforced epoxy matrix (CFRP). Two different silane coupling agents, (3-Aminopropyl)trimethoxysilane (AM) and (3-Glycidyloxypropyl)trimethoxysilane (EP), were applied, through the spray deposition method, on th…
Metal-free organic dyes with di(1-benzothieno)[3,2-b:2′,3′-d]pyrrole as an auxiliary donor for efficient dye-sensitized solar cells: Effect of the mo…
2019
Abstract A series of novel di(1-benzothieno)[3,2-b:2′,3′-d]pyrrole (DBTP)-based organic dyes (WL5-10) with D-D-π-A or D-D-A-π-A configurations are designed and the relationship between the properties and molecular configurations of these organic dyes are studied systematically. WL5,7–8 with D-D-π-A configuration incorporating triphenylamine or phenothiazine as donor and DBTP as auxiliary donor and WL9-10 with D-D-A-π-A configuration incorporating benzothiadiazole as an auxiliary acceptor are synthesized to study the effect of the molecular engineering on the photovoltaic performance. WL5,7–8 exhibit similar absorption spectra and high molar extinction coefficient. Especially, the dye WL5 wi…
Dithienopyrrolobenzothiadiazole-based organic dyes for efficient dye-sensitized solar cells
2014
Four novel D–π–A metal-free organic dyes DTP1–4 containing a dithienopyrrolobenzothiadiazole (DTPBT) unit were synthesized and applied in dye-sensitized solar cells, where DTPBT was employed as a π-spacer for the first time. The photophysical, electrochemical and photovoltaic properties of the dyes were systematically investigated. The dyes DTP1–4 showed broad absorption spectra and high molar extinction coefficient, resulting in high light harvesting efficiency. In addition, the impacts of donors and the thiophene unit as an additional π-spacer were also studied. The results showed that the dye DTP4 with triphenylamine as the donor exhibited better photovoltaic performance than DTP1–3 with…
Anti-recombination organic dyes containing dendritic triphenylamine moieties for high open-circuit voltage of DSSCs
2013
Abstract Three novel sensitizers with dendritic triphenylamine moieties were synthesized and used for dye-sensitized solar cells (DSSCs). Their absorption spectra, electrochemical and photovoltaic properties were extensively investigated. All three DSSCs exhibit high open-circuit voltage over 0.8 V. The photovoltaic results indicate that the dendritic triphenylamine units improve the open-circuit voltage, which is attributed to the retardation of charge recombination, demonstrating that non-planar and larger molecules exert better blocking function. Under standard global AM 1.5 solar conditions, the best performance of the DSSCs exhibits a short-circuit current density of 10.35 mA cm−2, an …