Search results for "EB"

Leucocyte Rheology at Baseline and after Activation in Post-Phlebitic Syndrome

1999

Objective: To evaluate leucocyte rheology, expressed as leucocyte filtration, polymorphonuclear leucocyte (PMN) membrane fluidity and cytosolic Ca2+ concentration in subjects with post-phlebitic leg syndrome (PPS). Methods: In 22 subjects with PPS we determined leucocyte filtration [unfractionated, mononuclear (MN) and PMN cells], employing the St George Filtrometer, PMN membrane fluidity using the fluorescent probe 1-[4-(trimethylamino)phenyl]-6-phenyl-1,3,5-hexatriene (TMA-DPH) and PMN cytosolic Ca2+ concentration using the fluorescent probe Fura 2-AM. Subsequently we determined the same PMN parameters after in vitro activation with 4-phorbol 12-myristate 13-acetate (PMA) and N-formyl-met…

Chronic cerebrospinal venous insufficiency theory after the Brave Dreams study

2018

Ancient cattle genomics, origins, and rapid turnover in the Fertile Crescent

2019

Cattle were domesticated ∼10,000 years ago, but analysis of modern breeds has not elucidated their origins. Verdugo et al. performed genome-wide analysis of 67 ancient Near Eastern Bos taurus DNA samples. Several populations of ancient aurochs were progenitors of domestic cows. These genetic lineages mixed ∼4000 years ago in a region around the Indus Valley. Interestingly, mitochondrial analysis indicated that genetic material likely derived from arid-adapted Bos indicus (zebu) bulls was introduced by introgression.Science, this issue p. 173Genome-wide analysis of 67 ancient Near Eastern cattle, Bos taurus, remains reveals regional variation that has since been obscured by admixture in mode…

Localization microscopy of DNA in situ using Vybrant(®) DyeCycle™ Violet fluorescent probe: A new approach to study nuclear nanostructure at single m…

2016

Higher order chromatin structure is not only required to compact and spatially arrange long chromatids within a nucleus, but have also important functional roles, including control of gene expression and DNA processing. However, studies of chromatin nanostructures cannot be performed using conventional widefield and confocal microscopy because of the limited optical resolution. Various methods of superresolution microscopy have been described to overcome this difficulty, like structured illumination and single molecule localization microscopy. We report here that the standard DNA dye Vybrant(®) DyeCycle™ Violet can be used to provide single molecule localization microscopy (SMLM) images of …

Inter- and intraspecific variability in invertebrate acute toxicity response to Arsenic and Fluoride exposure

2018

The adverse effects of arsenic and fluoride exposure on six groups of freshwater invertebrates were investigated. Acute toxicity tests (48-h) with arsenic trioxide (As2NO3) resulted in the following pattern of sensitivity: Daphnia magna 24-h-old = Brachionus patulus 72-h-old = Daphnia. cf. prolata, 21-d-old = D. magna 5-d-old > Heterocypris incongruens juvenile instars > Culex sp. Heterocypris juv. incongruens instars were the second group more tolerant to arsenic and the second group that bioconcentrates arsenic the least. In contrast, invertebrates exposed to sodium fluoride (NaF), showed a different pattern of sensitivity: H. incongruens instars > B. patulus = D. magna 24-h-old > D. cf. …

Roles for RpoS in survival of Escherichia coli during protozoan predation and in reduced moisture conditions highlight its importance in soil environ…

2017

The soil is a complex ecosystem where interactions between biotic and abiotic factors determine the survival and fate of microbial inhabitants of the system. Having previously shown that Escherichia coli requires the general stress response regulator, RpoS, to survive long term in soil, it was important to determine what specific conditions in this environment necessitate a functional RpoS. This study investigated the susceptibility of soil-persistent E. coli to predation by the single-celled eukaryotes Acanthamoeba polyphaga and Tetrahymena pyriformis, and the role RpoS plays in resisting this predation. Strain-specific differences were observed in the predation of E. coli strains, with so…

Novel Autotrophic Organisms Contribute Significantly to the Internal Carbon Cycling Potential of a Boreal Lake

2018

ABSTRACT Oxygen-stratified lakes are typical for the boreal zone and also a major source of greenhouse gas emissions in the region. Due to shallow light penetration, restricting the growth of phototrophic organisms, and large allochthonous organic carbon inputs from the catchment area, the lake metabolism is expected to be dominated by heterotrophic organisms. In this study, we test this assumption and show that the potential for autotrophic carbon fixation and internal carbon cycling is high throughout the water column. Further, we show that during the summer stratification carbon fixation can exceed respiration in a boreal lake even below the euphotic zone. Metagenome-assembled genomes an…

Associations between caloric compensation ability and eating behaviour in infants

2018

Bidimensional Structure of the Orthorexia: Development and Initial Validation of a New Instrument

2018

La ortorexia nerviosa puede entenderse como una preocupa- ción extrema o excesiva con alimentarse de comida considerada sana. Se puede distinguir entre ortorexia nerviosa y ortorexia saludable (interés en comer sano). Hasta ahora, no hay ningún instrumento disponible para eva- luar cada aspecto de la ortorexia con suficientes garantías psicométricas. El objetivo de este estudio era doble. Primero, desarrollar y validar un nuevo cuestionario de ortorexia –la Teruel Orthorexia Scale– y, segundo, analizar la asociación con otros contructos psicológicos y trastornos teóricamente relacionados con la ortorexia nerviosa: síntomas de trastornos de la alimen- tación, síntomas de trastorno obsesivo-c…

Antiinflammatory Therapy with Canakinumab for Atherosclerotic Disease

2017

Background: Experimental and clinical data suggest that reducing inflammation without affecting lipid levels may reduce the risk of cardiovascular disease. Yet, the inflammatory hypothesis of atherothrombosis has remained unproved. Methods: We conducted a randomized, double-blind trial of canakinumab, a therapeutic monoclonal antibody targeting interleukin-1β, involving 10,061 patients with previous myocardial infarction and a high-sensitivity C-reactive protein level of 2 mg or more per liter. The trial compared three doses of canakinumab (50 mg, 150 mg, and 300 mg, administered subcutaneously every 3 months) with placebo. The primary efficacy end point was nonfatal myocardial infarction, …