Search results for "ELISA"

Theoretical study on the molecular mechanism of the [5 + 2] vs. [4 + 2] cyclization mediated by Lewis acid in the quinone system

2013

[EN] The thermal and Lewis acid (LA) catalyzed cyclizations of quinone 1 involved in the synthesis of Colombiasin A and Elipsaterosin B have been theoretically studied using DFT methods at the B3LYP/6-311G(d,p) computational level. B3LYP calculations suggest that the formal endo [4 + 2] cycloadduct allowing the synthesis of Colombiasin A is preferentially formed under thermal conditions, while in the presence of the BF3 LA catalyst the formal [5 + 2] cycloadduct is seen, allowing the synthesis of Elipsaterosin B. The BF3 LA catalyst not only accelerates the nucleophilic attack on the C2 carbon of the quinone framework through a more polar C-C bond formation, but also provokes a different el…

Historia verdadera, y exemplar del Gran Cisma de Inglaterra, por el Rey Eduardo, y la impia Isabela, Sacada de los refereidos Autores, con Camdeno, y…

Data presa de Palau Sign.: [A]-B2, C[8 Reclams Grav. xil. a la port. avant del tít., representant els reis Frisos

Elisabethin perintö

2018

"Od Wmci Pani już bardzo dawno miałem wiadomość, proszę, abyś mi doniosła o zdrowiu swoim i obrotach...". Wojenne doświadczenia Elżbiety z Branickiej…

2018

Elżbieta z domu Branicka była od 1732 roku trzecią żoną Jana Tarły, wojewody lubelskiego, a od 1736 roku wojewody sandomierskiego. Korespondencja pomiędzy małżonkami wskazuje na dobre relacje, oparte o wzajemna troskę i zaufania. No to ostatnie Elżbieta w pełni zasługiwała za swoja postawę w latach 1733 - 1736 przetrwać trudne czasy wojny. W rzeczywistości stawiała czoła realnym, być może wyolbrzymionym zagrożeniom. Uwypukliło to takie cechy jej charakteru, jak zdecydowanie, zaradność, i odporność na sytuacje kryzysowe. Elżbieta z Branickich Tarłowa potrafiła dbać o siebie, męża i majątek rodzinny.

Immunoreagent generation and competitive assay development for cyprodinil analysis.

2012

Cyprodinil is an anilinopyrimidine fungicide applied worldwide for the prevention and treatment of highly destructive plant diseases in a large variety of crops, including cereals, fruits, and vegetables. This paper describes the development of the first reported immunoassays for cyprodinil. Two original haptens have been synthesized and conjugated to different carrier proteins, and polyclonal antibodies have been produced. Moreover, competitive enzyme-linked immunosorbent assays have been developed and characterized for the analysis of this widely used pesticide. The influence of organic solvents and buffer conditions over the assay analytical parameters was studied. The IC 50 values of th…

Using fractal objects as primitives for spatial modelling

2019

ECTQG 2019, European Colloquium of Theoretical and Quantitative Geography, Mondorf-les-Bains, LUXEMBOURG, 05-/09/2019 - 09/09/2019

Estimation des performances, test et contrôle des systèmes cyber-physiques employant des réseaux de communication non idéaux

2020

Wireless technology is a key enabler of the promises of Industry 4.0 (Smart Manufacturing). As such, wireless technology will be adopted as a principal mode of communication within the factory beginning with the factory enterprise and eventually being adopted for use within the factory workcell. Factory workcell communication has particular requirements on latency, reliability, scale, and security that must first be met by the wireless communication technology used. Wireless is considered a non-ideal form of communication in that when compared to its wired counterparts, it is considered less reliable (lossy) and less secure. These possible impairments lead to delay and loss of data in indus…

Fungicide multiresidue monitoring in international wines by immunoassays

2015

24 pages, 5 figures, 1 table. -- Available online 10 November 2015.

First report of grapevine fleck virus in vineyards of Bosnia and Herzegovina

2020

Grapevine is one of the most important fruit crops in Bosnia and Herzegovina with an annual production of around 40 thousand tons in 2018 (FAOSTAT, http://www.fao.org/ faostat/en/#data/QC). Production of grapevine in the country is mainly concentrated in vineyards in the Lištica and Mostar regions of Herzegovina with the two predominant autochthonous cultivars ‘Žilavka’ and ‘Blatina’. Grapevine fleck virus (GFkV) from the genus Maculavirus in the family Tymoviridae is a phloem limited virus with a positive-sense single-stranded RNA genome (Dreher et al. 2012). GFkV is associated with fleck disease of Vitis spp. In order to investigate the current status of GFkV in Bosnia and Herzegovina, 80…

Highly sensitive monoclonal antibody-based immunoassays for the analysis of fluopyram in food samples

2019

Monoclonal antibody-based techniques have become a useful analytical technology in the agro-food sector. Nowadays, residues of the recently registered fungicide fluopyram are increasingly being found in quality control programs. In the present study, novel chemical derivatives of this pesticide were prepared and specific and high-affinity monoclonal antibodies to fluopyram were raised for the first time. Moreover, immunoassays to fluopyram were developed in two alternative enzyme-linked immunosorbent assay formats, using homologous and heterologous assay conjugates, with limits of detection below 0.05 µg L−1. The optimized immunoassays were applied to the analysis of fluopyram in fortified …