Search results for "ENTRY"
showing 10 items of 211 documents
A Bimolecular Multicelular complementation system for the detection of syncytium formation: A new methodology for the identification of entry inhibit…
2019
AbstractFusion of viral and cellular membranes is a key step during the viral life cycle. Enveloped viruses trigger this process by means of specialized viral proteins expressed on their surface, the so called viral fusion proteins. There are multiple assays to analyze the viral entry including those that focus on the cell-cell fusion induced by some viral proteins. These methods often rely on the identification of multinucleated cells (syncytium) as a result of cell membrane fusions. In this manuscript, we describe a novel methodology for the study of cell-cell fusion. Our approach, named Bimolecular Multicelular Complementation (BiMuC), provides an adjustable platform to investigate quali…
Rappresentazioni del potere. Le cerimonie dedicate al duca di Maqueda durante la sua permanenza in Sicilia (1598-1601)
2013
Attraverso le carte del Cerimoniale del Protonotaro del Regno è possibile ricostruire alcuni fattori necessari affinché si possa dare testimonianza di una effettiva commistione tra il Governo spagnolo e la Nobiltà siciliana. A tale proposito è sembrato doveroso sottrarre tali carte dal solo ambito folklorico e delle tradizioni popolari e affidarle ad una rilettura, ovviando all’esiguo interesse degli storici che tali documenti avevano relegato a semplici “esempi di spreco e vanità”. Dobbiamo invece individuarle come strumento per seguire la vita di un complesso quadro sociale che ha coinvolto personaggi dell’aristocrazia titolata e della nobiltà minore, dei patriziati urbani e delle oligarc…
Spatial Competition in Quality
2011
We develop a model of vertical innovation in which firms incur a market entry cost and position themselves in the quality space. Once established, firms compete monopolistically, selling to consumers with heterogeneous tastes for quality. We establish the general existence and conditional uniqueness of the pricing game in such vertically differentiated markets with a potentially large number of active firms. Turning to firms’ entry decisions, exogenously growing productivities induce firms to enter the market sequentially at the top end of the quality spectrum. We spell out the conditions under which the entry problem is replicated over time so that each new entrant improves incumbent quali…
Next Generation Home Sharing: Disrupting Platform Organizations with Blockchain Technology and the Internet of Things?
2020
During the last decade, the sharing economy has given birth to a number of market mediators, which have grown to become the world’s most valuable companies. Centralized sharing platforms like Uber, Didi Chuxing, and Airbnb have transformed several traditional industries. However, with the rise of the blockchain technology, some voices predict that these platform companies will soon be at risk of being disrupted themselves. In this chapter, we address the question of whether blockchain technology has given birth to a new breed of sharing economy platforms that can challenge the incumbents’ business models. We identify five different stages of the application of blockchain technology in emerg…
Interpretation of epicardial mapping by means of computer simulations: Applications to calcium, lidocaine and to BRL 34915
1992
The aim of this work was to compare experimental investigations on effects of lidocaine, calcium and, BRL 34915 on reentries to simulated data obtained by use of a model of propagation based on the Huygens' construction method already described in previous works. Calcium and lidocaine effects are investigated on anisotropic conduction conditions. In both cases, reduction in conduction velocities are observed. In lidocaine case, a refractory area is located along the longitudinal axis. In agreement with experimental electrical mapping, the simulations show that the stabilization of reentrant excitation is mainly due to the existence of this refractory area around which the reentrant circuit …
El arquitecto Rafael Guastavino (1842-1908): obra en cuatro actos
2018
[EN] This text presents extensive new information on the figure of architect Rafael Guastavino Moreno (1842-1908), those around him, and his personal and family relationships with architecture. It also examines other artistic fields and trades such as music, carpentry and winemaking, all of which influenced and obtained feedback for Guastavino¿s architecture within the historical, political and economic situation of the three places where he lived in Spain: Valencia, Barcelona and Almudévar (Huesca), before emigrating to the United States of America, where his successful professional career as an architect is widely recognized in the literature
Monitoring of Plasma Concentrations of Psychotropic Drugs in Pharmacovigilance
2015
The primary aims of pharmacovigilance are supervision and prevention of medication-related problems under everyday conditions. Pharmacovigilance is an indication for monitoring plasma concentrations, i.e. conducting therapeutic drug monitoring (TDM). Using TDM, it can be clarified if observed unwanted drug effects may be attributed to abnormally high or low drug concentrations. Utmost benefits from TDM are obtained for pharmacovigilance when the method is adequately integrated into the clinical treatment process. How to do this is described in consensus guidelines for TDM in psychiatry. During the last 20 years, TDM was very successful for detection of multiple pharmacokinetic drug-drug int…
Echovirus 1 Entry into Polarized Caco-2 Cells Depends on Dynamin, Cholesterol, and Cellular Factors Associated with Macropinocytosis
2013
ABSTRACT Enteroviruses invade their hosts by crossing the intestinal epithelium. We have examined the mechanism by which echovirus 1 (EV1) enters polarized intestinal epithelial cells (Caco-2). Virus binds to VLA-2 on the apical cell surface and moves rapidly to early endosomes. Using inhibitory drugs, dominant negative mutants, and small interfering RNAs (siRNAs) to block specific endocytic pathways, we found that virus entry requires dynamin GTPase and membrane cholesterol but is independent of both clathrin- and caveolin-mediated endocytosis. Instead, infection requires factors commonly associated with macropinocytosis, including amiloride-sensitive Na + /H + exchange, protein kinase C, …
La inseparabilidad y el grado de adaptación como determinantes de la internacionalización de las empresas de servicios
2005
-Esther.Sanchez@uv.es -Jose.Pla@uv.es La mayor parte de enfoques teóricos de internacionalización han surgido en el marco del sector industrial y, por tanto, algunas explicaciones podrían no ser generalizables al sector servicios en su globalidad. Por ello, las características sectoriales deben ser tenidas en cuenta en el análisis de los modos de entrada de las empresas de servicios, ya que es posible encontrar patrones de comportamiento muy diferentes no sólo con respecto a las empresas industriales sino también entre las propias empresas de servicios. En este trabajo se proponen la inseparabilidad de los servicios y el grado de adaptación a las necesidades del cliente como dimensiones cla…
Early entry events in Echovirus 30 infection
2020
Echovirus 30 (E30), a member of the enterovirus B species, is a major cause of viral meningitis, targeting children and adults alike. While it is a frequently isolated enterovirus and the cause of several outbreaks all over the world, surprisingly little is known regarding its entry and replication strategy within cells. In this study, we used E30 strain Bastianni (E30B) generated from an infectious cDNA clone in order to study early entry events during infection in human RD cells. E30B required the newly discovered Fc echovirus receptor (FcRn) for successful infection, but not the coxsackievirus and adenovirus receptor (CAR) or decay-accelerating factor (DAF), although an interaction with …