Search results for "ENZYMES"

showing 10 items of 730 documents

The fnr Gene of Bacillus licheniformis and the Cysteine Ligands of the C-Terminal FeS Cluster

1998

Many of the O2-responsive gene regulators of bacteria are members of the fumarate nitrate reductase-cyclic AMP receptor protein family of transcriptional regulators (12, 13, 15, 17) with predicted structures similar to those of the cyclic AMP receptor protein (11). The Fnr (stands for fumarate nitrate reductase regulator) protein from Escherichia coli (FnrEc) controls the expression of a variety of genes, mainly of anaerobic respiration and metabolism (5, 13). It contains a N-terminal cluster of three essential cysteine residues which are supposed to bind together with Cys122 a [4Fe 4S]2+ cluster which is required for O2 sensing (4, 7, 8, 10, 16). A wide variety of gram-negative bacteria co…

inorganic chemicalsIron-Sulfur ProteinsMolecular Sequence DataRestriction MappingMutantBacillusGenetics and Molecular BiologySequence alignmentmacromolecular substancesBacillus subtilisLigandsNitrate reductaseenvironment and public healthMicrobiologyBacterial ProteinsAmino Acid SequenceCysteineBacillus licheniformisMolecular BiologyPeptide sequenceBacillus megateriumSequence Homology Amino AcidbiologyEscherichia coli ProteinsGene Expression Regulation Bacterialbiology.organism_classificationenzymes and coenzymes (carbohydrates)KineticsBiochemistryBacillus megateriumbacteriaSequence AlignmentBacillus subtilisTranscription FactorsCysteineJournal of Bacteriology
researchProduct

The Myristoylated Alanine-Rich C-Kinase Substrate (MARCKS) is Sequentially Phosphorylated by Conventional, Novel and Atypical Isotypes of Protein Kin…

1995

The myristoylated alanine-rich C-kinase substrate (MARCKS) is the major protein kinase C (PKC) substrate in many cell types including fibroblasts and brain cells. Here we describe the phosphorylation of MARCKS and the site specificity for different PKC isotypes. Conventional (c)PKC beta 1, novel (n)PKC delta and nPKC epsilon efficiently phosphorylated the MARCKS protein in vitro. The Km values were extremely low, reflecting a high affinity between kinases and substrate. The apparent affinity of nPKC delta (Km = 0.06 microM) was higher than that of nPKC epsilon and cPKC beta 1 (Km = 0.32 microM). The rate of substrate phosphorylation was inversely correlated with affinity and decreased in th…

inorganic chemicalsKinaseChemistryIntracellular Signaling Peptides and ProteinsMembrane ProteinsProteinsContext (language use)macromolecular substancesenvironment and public healthBiochemistryMolecular biologyCell biologyIsoenzymesSerineKineticsenzymes and coenzymes (carbohydrates)Substrate-level phosphorylationbacteriaPhosphorylationPhosphorylationMARCKSMyristoylated Alanine-Rich C Kinase SubstrateProtein Kinase CProtein kinase CMyristoylationEuropean Journal of Biochemistry
researchProduct

Solvothermal Synthesis of Molybdenum–Tungsten Oxides and Their Application for Photoelectrochemical Water Splitting

2018

Molybdenum and tungsten oxides are of interest as semiconductors for the production of clean and sustainable energy. Here we show that synergistic effects arising from a combination of noncrystalli...

inorganic chemicalsMaterials scienceGeneral Chemical EngineeringSolvothermal synthesisMolybdenum oxidechemistry.chemical_element02 engineering and technologyTungsten010402 general chemistry01 natural sciencesEnvironmental ChemistryRenewable Energy Sustainability and the Environmentbusiness.industrytechnology industry and agricultureTungsten oxideGeneral Chemistryequipment and supplies021001 nanoscience & nanotechnology0104 chemical sciencesSustainable energyenzymes and coenzymes (carbohydrates)SemiconductorchemistryChemical engineeringMolybdenumbacteriaWater splitting0210 nano-technologybusinessACS Sustainable Chemistry & Engineering
researchProduct

Molybdenum blue: Binding to collagen fibres and microcrystal formation

2005

Collagen fibres have been shown by transmission electron microscopy to progressively bind the polyoxomolybdate ring-complex, termed molybdenum blue. Nucleation of cuboidal molybdenum blue microcrystals occurs on the surface of the collagen fibres, leading eventually to extensive coating of the fibres with microcrystals.

inorganic chemicalsMaterials scienceInorganic chemistryNucleationGeneral Physics and Astronomyengineering.materialMicroscopy Electron TransmissionCoatingMolybdenum blueStructural BiologyCollagen fibresAnimalsGeneral Materials ScienceMuscle SkeletalMolybdenumAmmonium molybdateStaining and LabelingCell BiologyCollagen fibreenzymes and coenzymes (carbohydrates)Chemical engineeringTransmission electron microscopyengineeringbacteriaCollagenCrystallizationMicron
researchProduct

Truncated recombinant light harvesting complex II proteins are substrates for a protein kinase associated with photosystem II core complexes

1998

AbstractPrevious studies directed towards understanding phosphorylation of the chlorophyll a/b binding proteins comprising light harvesting complex II (LHC II) have concentrated on a single phosphorylation site located close to the N-terminus of the mature proteins. Here we show that a series of recombinant pea Lhcb1 proteins, each missing an N-terminal segment including this site, are nevertheless phosphorylated by a protein kinase associated with a photosystem II core complex preparation. An Lhcb1 protein missing the first 58 amino acid residues is not, however, phosphorylated. The results demonstrate that the LHC II proteins are phosphorylated at one or more sites, the implications of wh…

inorganic chemicalsPhotosystem IIMacromolecular SubstancesMolecular Sequence DataPhotosynthetic Reaction Center Complex ProteinsLight-Harvesting Protein ComplexesBiophysicsmacromolecular substancesBiologyBiochemistryDNA-binding proteinProtein kinaseThylakoid membraneSubstrate Specificitylaw.inventionStructural BiologylawGeneticsProtein phosphorylationAmino Acid SequencePhosphorylationProtein kinase AMolecular BiologyPlant ProteinsKinasePeasPeaPhotosystem II Protein Complexfood and beveragesCell BiologySpinachPeptide FragmentsRecombinant Proteinsenzymes and coenzymes (carbohydrates)BiochemistryThylakoidRecombinant DNALight harvesting proteinPhosphorylationbacteriaCarrier ProteinsProtein KinasesFEBS Letters
researchProduct

Role of glutathione in the formation of the active form of the oxygen sensor FNR ([4Fe-4S]·FNR) and in the control of FNR function

2000

The oxygen sensor regulator FNR (fumarate nitrate reductase regulator) of Escherichia coli is known to be inactivated by O2 as the result of conversion of a [4Fe-4S] cluster of the protein into a [2Fe-2S] cluster. Further incubation with O2 causes loss of the [2Fe-2S] cluster and production of apoFNR. The reactions involved in cluster assembly and reductive activation of apoFNR isolated under anaerobic or aerobic conditions were studied in vivo and in vitro. In a gshA mutant of E. coli that was completely devoid of glutathione, the O2 tension for the regulatory switch for FNR-dependent gene regulation was decreased by a factor of 4–5 compared with the wild-type, suggesting a role for glutat…

inorganic chemicalsReducing agentCysteine desulfuraseMutantRegulatormacromolecular substancesGlutathioneBiologymedicine.disease_causeNitrate reductaseenvironment and public healthBiochemistryenzymes and coenzymes (carbohydrates)chemistry.chemical_compoundchemistryBiochemistrymedicinebacteriaEscherichia coliCysteineEuropean Journal of Biochemistry
researchProduct

Studying the phosphoryl transfer mechanism of the

2019

Phosphofructokinases catalyze the ATP-dependent phosphorylation of fructose-6-phosphate and they are highly regulated.

inorganic chemicalsenzymes and coenzymes (carbohydrates)Chemistrybacteriamacromolecular substancesenvironment and public healthChemical science
researchProduct

Retinal Microcirculation in Type 1 Diabetic Patients With and Without Peripheral Sensory Neuropathy

2014

In patients with diabetes mellitus (DM), early retinal microvascular alterations can be observed even before the clinical diagnosis of diabetic retinopathy. This study aimed to investigate morphological and functional changes in retinal microvascular blood flow in type 1 diabetic patients with and without peripheral neuropathy (PNP) as compared to nondiabetic controls. Retinal microvascular blood flow (RBF) was assessed using scanning laser Doppler flowmetry (Heidelberg Retina Flowmeter, Heidelberg Engineering, Germany) before and after stimulation with flicker light. PNP was assessed using the neuropathy disability score (NDS) and by the evaluation of the vibration perception threshold (V…

inorganic chemicalsmedicine.medical_specialtyEndocrinology Diabetes and MetabolismBiomedical EngineeringBioengineering-chemistry.chemical_compoundVibration perceptionMedizinische FakultätDiabetes mellitusOphthalmologyInternal Medicinemedicineheterocyclic compoundsddc:610RetinaType 1 diabetesbusiness.industryRetinalBlood flowDiabetic retinopathyOriginal Articlesmedicine.diseaseSurgeryenzymes and coenzymes (carbohydrates)Peripheral neuropathymedicine.anatomical_structurechemistrybusiness
researchProduct

In Vitro and In Vivo Regulation of SRD5A mRNA Expression of Supercritical Carbon Dioxide Extract from Asparagus racemosus Willd. Root as Anti-Sebum a…

2022

Oily skin from overactive sebaceous glands affects self-confidence and personality. There is report of an association between steroid 5-alpha reductase gene (SRD5A) expression and facial sebum production. There is no study of the effect of Asparagus racemosus Willd. root extract on the regulation of SRD5A mRNA expression and anti-sebum efficacy. This study extracted A. racemosus using the supercritical carbon dioxide fluid technique with ethanol and investigated its biological compounds and activities. The A. racemosus root extract had a high content of polyphenolic compounds, including quercetin, naringenin, and p-coumaric acid, and DPPH scavenging activity comparable to that of the standa…

integumentary systemChemistry (miscellaneous)Organic ChemistryDrug DiscoveryMolecular MedicinePharmaceutical SciencePellPhysical and Theoretical Chemistryanti-sebum efficacy; <i>Asparagus racemosus</i>; facial-pore-minimizing efficacy; facial sebum production; 5-alpha reductase enzymes; human volunteer; oily skin; polyphenols; <i>SRD5A</i>; supercritical carbon dioxide fluid extractionPlantes medicinalsAndrògensAnalytical ChemistryMolecules
researchProduct

Oxidative Stress and DNA Damage in Human Gastric Carcinoma: 8-Oxo-7&#039;8-dihydro-2&#039;-deoxyguanosine (8-oxo-dG) as a Possible Tumor Marker

2013

We characterized the oxidative stress (OS) status by the levels of reduced/oxidized glutathione (GSH/GSSG), malondialdehyde (MDA) and the mutagenic base 8-oxo-7&#039;8-dihydro-2&#039;-deoxyguanosine (8-oxo-dG) in human gastric carcinoma (HGC) samples and compared the results with normal tissue from the same patients. We also analyzed 8-oxo-dG in peripheral mononuclear cells (PMNC) and urine from healthy control subjects and in affected patients in the basal state and one, three, six, nine and twelve months after tumor resection. The levels of DNA repair enzyme mRNA expression (hOGG1, RAD51, MUYTH and MTH1) were determined in tumor specimens and compared with normal mucosa. Tumor specimens e…

lcsh:ChemistryDNA repair enzymeslcsh:Biology (General)lcsh:QD1-999gastric cancertumor markeroxidative stress8-oxo-dGlcsh:QH301-705.5International Journal of Molecular Sciences
researchProduct